数字农科院2.0

Effect of Methionine Supplementation on Serum Metabolism and the Rumen Bacterial Community of Sika Deer (Cervus nippon)

文献类型： 外文期刊

作者： Wu Y.; Guo X.; Zhao D.; Xu C.; Sun H.; Yang Q.; Wei Q.; Si H.; Wang K.; Zhang T.

作者机构：

关键词： glutathione peroxidase; immunoglobulin A; immunoglobulin G; immunoglobulin M; methionine; protein; serum albumin; serum globulin; superoxide dismutase; agar gel electrophoresis; alkaline phosphatase blood level; animal experiment; Article; aspartate aminotransferase blood level; Bacteroidetes; centrifugation; cholesterol blood level; colorimetry; controlled study; diet supplementation; DNA extraction; DNA sequencing; enzyme linked immunosorbent assay; fermentation; Firmicutes; gene amplification; glucose blood level; high density lipoprotein cholesterol level; low density lipoprotein cholesterol level; male; metabolism; microbial community; nonhuman; polymerase chain reaction; principal coordinate analysis; Proteobacteria; real time polymerase chain reaction; rumen; sika deer; triacylglycerol blood level

期刊名称： Animals

ISSN： 2076-2615

年卷期： 2022 年 12 卷 15 期

页码：

收录情况： SCIE（2022版）

摘要： Methionine is the first or second limiting amino acid for ruminants, such as sika deer, and has a variety of biological functions such as antioxidant activity, immune response, and protein synthesis. This study aimed to investigate the effects of methionine supplementation on antler growth, serum biochemistry, rumen fermentation, and the bacterial community of sika deer during the antler-growing period. Twelve 4-year-old male sika deer were randomly assigned to three dietary groups supplemented with 0 g/day (n = 4, CON), 4.0 g/day (n = 4, LMet), and 6.0 g/day (n = 4, HMet) methionine. No significant difference (p > 0.05) was found in the production performance between the three groups, but antler weight was higher in both the LMet and HMet groups than in the CON group. Methionine supplementation significantly increased the serum glutathione peroxidase activity (p < 0.05). The serum immunoglobulin G level was significantly higher in the HMet group than in the other two groups (p < 0.05). No significant effect was found on the apparent amino acid digestibility of the three groups, but cysteine and methionine digestibility were higher in the LMet group. The serum hydroxylysine level was significantly lower in the LMet and HMet groups, whereas the serum lysine level was significantly lower in the HMet group compared with the CON group (p < 0.05). The LMet group had the highest but a nonsignificant total volatile fatty acid content and significantly higher microbial protein content in the rumen than the CON group (p < 0.05). The phyla Bacteroidetes, Firmicutes, and Proteobacteria were dominant in the rumen of the sika deer. The principal coordinate analysis (PCoA) and analysis of similarities (ANOSIM) results showed a significant change in the bacterial composition of the three groups (p < 0.05). The relative abundance of Prevotella and Rikenellaceae-RC9 was significantly higher in the LMet group compared with the CON group and CON and HMet groups, respectively. These results revealed that methionine supplementation improved the antioxidant activity and immune status, affecting amino acid metabolism and rumen microbial composition of the sika deer. © 2022 by the authors.

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