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Nucleophosmin 1 inhibits the replication of influenza A virus by competitively binding viral RNA with viral proteins

文献类型： 外文期刊

作者： Yu, Yingying;Wang, Qian;Wei, Yanli;Liu, Junwen;Wang, Guangwen;Wang, Zhengxiang;Shen, Wentao;Han, Lu;Li, Chengjun;Lei, Cao-Qi;Xu, Shuai;Zhu, Qiyun

作者机构：

关键词： RNA binding;Viral RNA;Viral polymerase;Nucleophosmin-1 (NPM1);Influenza A virus (IAV)

期刊名称： VIROLOGICA SINICA

ISSN： 1674-0769

年卷期： 2025 年 40 卷 3 期

页码：

收录情况： SCIE（2025版） ; ; CSCD（2025-2026年度） ; ; 科技核心（2024版）

摘要： Influenza A viruses (IAVs) are single-stranded negative-sense RNA viruses that continually challenge animal and human health. In IAV-infected cells, host RNA-binding proteins play key roles in the life cycle of IAV by directly binding to viral RNA. Here, we examined the role of the host RNA-binding protein nucleophosmin-1 (NPM1) in IAV replication. We found that, as a nucleolar phosphoprotein, NPM1 directly binds to viral RNA (vRNA) and inhibits the replication of various subtypes of IAV. NPM1 binding to vRNA competitively reduces the assembly of the viral ribonucleoprotein complex and the viral polymerase activity, thereby reducing the generation of progeny viral RNA and virions. The RNA-binding activity of NPM1, with the key residues T199, T219, T234, and T237, is essential for its anti-influenza function. Taken together, our findings demonstrate that NPM1 acts as an RNA-binding protein and interacts with IAV vRNA to suppress viral replication.

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