数字农科院2.0

Discovery and vertical transmission analysis of Dabieshan Tick Virus in Haemaphysalis longicornis ticks from Chengde, China

文献类型： 外文期刊

作者： Xu, Xiaofeng;Gao, Zhihua;Wu, Youhong;Yin, Hong;Ren, Qiaoyun;Zhang, Jie;Liu, Yongsheng;Yang, Shunli;Bayasgalan, Chimedtseren;Tserendorj, Ariunaa;Yang, Xiaolong;Chen, Ze

作者机构：

关键词： Haemaphysalis longicornis;Dabieshan Tick Virus;ticks;transstadial transmission;transovarial transmission;Hebei Province

期刊名称： FRONTIERS IN MICROBIOLOGY

ISSN：

年卷期： 2024 年 15 卷

页码：

收录情况： SCIE（2024版）

摘要： Introduction: Ticks are important blood-sucking ectoparasites that can transmit various pathogens, posing significant threats to the wellbeing of humans and livestock. Dabieshan tick virus (DBTV) was initially discovered in 2015 in Haemaphysalis longicornis ticks from the Dabieshan mountain region in Hubei Province, China. In recent years, DBTV has been discovered in various regions of China, including Shandong, Zhejiang, Liaoning, Hubei, Yunnan, and Guizhou Provinces. However, the researches on tick-borne transmission of DBTV are scarce. Methods: This study utilized the small RNA sequencing (sRNA-seq) method to identify tick-associated viruses in ticks collected from Chengde in Hebei Province and Yongcheng in Henan Province, leading to the discovery of a new DBTV strain in Hebei. The complete coding genome of DBTV Hebei strain was obtained through RNA-seq and Sanger sequencing. Furthermore, the transmission experiment of DBTV in H. longicornis was examined in laboratory for the first time. Results: DBTV was detected in newly molted adult H. longicornis ticks collected in Chengde, Hebei Province. Additionally, DBTV was also detected in both unfed nymphs and engorged females of H. longicornis collected from Chengde, with a positive rate of 20% and 56.25%, respectively. The complete coding genome of DBTV (OP682840 and OP716696) were obtained, and phylogenetic analysis revealed that the DBTV Hebei strain clustered with previously reported DBTV strains. Furthermore, this virus was observed in engorged females, eggs, and larvae of the subsequent generation. Discussion: It is necessary to expand the scope of DBTV investigation, particularly in northern China. This study demonstrated that DBTV can be transmitted from engorged females to larvae of the next generation. Moreover, the detection of DBTV in unfed nymphs and adults (which moulted from engorged nymphs) collected from the filed of Chengde suggests that H. longicornis serves as a potential transmission host and reservoir for DBTV through transstadial and transovarial transmission. However, there remains a lack of research on the isolation and pathogenicity of DBTV, highlighting the need for further studies to mitigate potential harm to the health of animals and humans.

分类号：

  • 相关文献

[1]The Characteristic Analysis of Ribosomal Protein L12 in Haemaphysalis longicornis (Acari: Ixodidae) Ticks [Haemaphysalis longicornis (Akar: Ixodidae) Kenelerinde Ribozomal Protein L12’nin Karakteristik Analizi]. Jin Luo,Wenge Liu,Qiaoyun Ren,Xiaokai Song,Ruofeng Yan,Guangyuan Liu,Xiangrui Li. 2022

[2]Comparative Analysis Of Microrna Profiles B.etween Wild And Cultured H aemaphysalis Longicornis (Acari, Ixodidae) Ticks. Luo, J,Ren, QY,Chen, Z,Liu, WG,Qu, ZA,Xiao, RH,Chen, RG,Lin, HL,Wu, ZG,Luo, JX,Yin, H,Wang, H,Liu, GY. 2019

[3]First determination of diagnostic doses of the pesticide-sensitive strain of Haemaphysalis longicornis in China. Diao, Peiwen,Luo, Jin,Ren, Qiaoyun,Wang, Jinming,Cao, Runlai,Zhang, Yan,Guo, Junhui,Guan, Guiquan,Luo, Jianxun,Yin, Hong,Liu, Guangliang,Liu, Guangyuan. 2025

[4]Comparative metagenomic profiling of microbial pathogen diversity in Haemaphysalis longicornis and Hyalomma dromedarii ticks. Jin, Luo,Lan, Xinting,Zhao, Shuaiyang,Ren, Qiaoyun,Obaid, Muhammad Kashif,Diao, Peiwen,Guan, Guiquan,Liu, Guangyuan,Hong, Yin. 2025

[5]Yield estimation of winter wheat in North China Plain by using crop growth monitoring system (CGMS). Teng Fei,Chen Zhongxin,Wu Wenbin,Huang Qing,Li Dandan,Xia Tian. 2012

[6]Farmers Preferred Information Sources for Agricultural Productivity in Hebei Province, China. Muhammad Yaseen,Xu Shiwei,Yu Wen,Muhammad Luqman,Raheel Saqib,Muhammad Ameen,Sadia Hassan,Tahir Munir Butt. 2021

[7]Farmers Preferred Information Sources for Agricultural Productivity in Hebei Province, China. Muhammad Yaseen,Xu Shiwei,Yu Wen,Muhammad Luqman,Raheel Saqib,Muhammad Ameen,Sadia Hassan,Tahir Munir Butt. 2021

[8]Technical Efficiency of Maize Production and Its Influencing Factors in the World’s Largest Groundwater Drop Funnel Area, China. Zhaohong Wu,Wenyuan Hua,Liangguo Luo,Katsuya Tanaka. 2022

[9]First report of heterodera filipjevi on winter wheat from hebei province in north china. Jiangkuan Cui,Haohao Ren,Bo Zhou,Kunyuan Chen,Deliang Peng,Minmin Li,Haoguang Meng,Shijun Jiang. 2021

[10]Detection of meteorological influence on bread wheat quality in Hebei province, China based on the gradient boosting decision tree. Zhang, Xinyue,Chen, Keyao,Li, Kuo. 2023

[11]Probabilistic assessment of water network influence zones and ecosystem responses following the middle route of the South-to-North Water Diversion Project. Jiang, Tianliang,Qu, Yanping,Zhang, Xuejun,Su, Dandan,Han, Yu,Zhao, Ruixuan. 2025

[12]Proteomic analysis of castor bean tick Ixodes ricinus: a focus on chemosensory organs. Dani, Francesca Romana,Ban, Liping,Song, Limei,Pelosi, Paolo,Dani, Francesca Romana.

[13]Comparative Profiling of MicroRNAs in Male and Female Rhipicephalus sanguineus. Shao, Chang-Chun,Tao, Jian-Ping,Xu, Min-Jun,Zhu, Xing-Quan,Chen, Yi-Zhou.

[14]The life cycle of Hyalomma rufipes (Acari: Ixodidae) under laboratory conditions. Chen, Ze,Li, Youquan,Liu, Zhijie,Yang, Jifei,Yin, Hong. 2012

[15]Molecular Evidence of Bartonella melophagi in Ticks in Border Areas of Xinjiang, China. Jun Ni,Qiaoyun Ren,Hanliang Lin,Malike Aizezi,Jin Luo,Yi Luo,Zhan Ma,Ze Chen,Wenge Liu,Junhui Guo,Zhiqiang Qu,Xiaofeng Xu,Zegong Wu,Yangchun Tan,Jinming Wang,Youquan Li,Guiquan Guan,Jianxun Luo,Hong Yin,Guangyuan Liu. 2021

[16]Tick distribution and detection of Babesia and Theileria species in Eastern and Southern Kazakhstan. Chunli Sang,Meihua Yang,Bin Xu,Guangyuan Liu,Yicheng Yang,Kenesbay Kairullayev,Otarbayev Bauyrzhan,Wurelihazi Hazihan,Sándor Hornok,Yuanzhi Wang. 2021

[17]Proteomics reveals the hemolymph components of partially fed Hyalomma asiaticum ticks. Yuan C.,Yang Q.,Wu J.,Peng Y.,Li Y.,Xiong S.,Zhou J.,Wang M.,Hu Z.,Zou Z.,Xia Q.. 2022

[18]Virome of Giant Panda-Infesting Ticks Reveals Novel Bunyaviruses and Other Viruses That Are Genetically Close to Those from Giant Pandas. Ma, Rui,Zhao, Min,Wang, Haoning,Hou, Rong,Qin, Kailin,Qian, Yu,Zhang, Han,Zhou, Yanshan,Wu, Wei,Gu, Jiang,Wang, Xiaochun,Shen, Quan,Liu, Songrui,Liu, Jiabin,Bi, Wenlei,Yu, Xiang,Yang, Shixing,Feng, Feifei,Li, Zusheng,Zhang, Long,Lan, G. 2022

[19]Characterization of an MLP Homologue from Haemaphysalis longicornis (Acari: Ixodidae) Ticks. Luo, Jin,Luo, Jin,Chen, Ronggui,Yin, Hong,Zhao, Bo,Zhao, Hongying,Shen, Hui,Li, Xiangrui,Ren, Qiaoyun,Guan, Guiquan,Yin, Hong,Luo, Jianxun,Liu, Guangyuan. 2020

[20]Epidemiological Survey Of Ticks And T.ick-Borne Pathogens In Pet D ogs In South-Eastern China. Zhang, JW, Liu, QB, Wang, DM, Li, WM, Beugnet, F, Zhou, JL. 2017

作者其他论文 更多>>