数字农科院2.0

Untargeted metabolomics analysis of non-volatile metabolites and dynamic changes of antioxidant capacity in Douchi with edible mushroom by-products

文献类型： 外文期刊

作者： Rong, Pei-Xiu;He, Xiao-Qin;Ayyash, Mutamed;Liu, Yi;Wu, Ding-Tao;Geng, Fang;Li, Hua-Bin;Ng, Siew Bee;Liu, Hong-Yan;Gan, Ren-You

作者机构：

关键词： Singapore;Douchi;Fermentation;UPLC-MS/MS;Metabolomics;Antioxidant activity

期刊名称： FOOD CHEMISTRY

ISSN： 0308-8146

年卷期： 2024 年 431 卷

页码：

收录情况： SCIE（2024版） ; ; EI（2024版）

摘要： This study investigated the non-volatile metabolites and antioxidant activity of Douchi, an edible mushroom byproduct. A total of 695 non-volatile metabolites were detected using UPLC-MS/MS-based metabolomics analysis, and the greatest impact on metabolite composition was observed during Koji-making and the first 5 days of postfermentation. Throughout the fermentation process, 366 differential metabolites were identified, with flavonoids being the most prominent followed by amino acids and their derivatives, which were found to be important for the quality of edible mushroom by-product Douchi (EMD). The antioxidant capacity of EMD significantly increased with the longer fermentation time, which might be associated with the conversion of isoflavone glycosides to aglycones, amino acids and their derivatives, free fatty acids, group A saponins, and phenolic acids. These findings suggested that different fermentation phases of EMD significantly affected the non-volatile metabolite profile and antioxidant capacity.

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