数字农科院2.0

Elevated EBF2 in mouse but not pig drives the progressive brown fat lineage specification via chromatin activation

文献类型： 外文期刊

作者： Liao, Yinlong;Peng, Zhelun;Fu, Shanshan;Hua, Yao;Luo, Wenzhe;Liu, Ruige;Chen, Yingjin;Gu, Wei;Zhao, Pengxiang;Zhao, Jianguo;Wang, Yanfang;Wang, Heng

作者机构：

关键词： Brown adipose tissue;Chromatin modification;Ebf2;Pig;Mouse

期刊名称： JOURNAL OF ADVANCED RESEARCH

ISSN： 2090-1232

年卷期： 2025 年 76 卷

页码：

收录情况： SCIE（2025版）

摘要： Brown adipose tissue (BAT) mals, including pigs. Duri expressing somitic mesode mechanisms underlying the fate decisions between brown fat and muscle progenitors remain elusive across species. In this study, we analyzed the dynamics of chromatin landscape during the segregation and specification of brown fat and muscle lineages from Pax7+ multipotent mesodermal stem cells, aiming to uncover epigenetic f actors that drive de novo BAT formation. Notably, myogenic progenitors were specified at embryonic day related genes. In contrast, step-wise depositions of hi fied the transcription factor early B-cell factor 2 (EBF2) as a key driver of the progressive specification of brown fat lineage and the simultaneous deviation away from the muscle lineage. Mechanistically, EBF2 interacts with transcriptional co-activators CREB binding protein/ E1A-binding protein p300 (CBP/P300) to induce H3K27ac deposi is responsible for non-shivering thermogenesis, but it is absent in some mamng development, BAT progenitors are derived from paired box 7 (Pax7)-rmal stem cells, which also give rise to skeletal muscle. However, the intrinsic (E) 12.5, exhibiting high levels of H3K4me3 and low H3K27me3 at muscle-the specification of the BAT lineage occurred much later, with coordinated stone modifications at BAT-associated genes from E10.5 to E14.5. We identition and chromatin activation at BAT-associated genes to promote brown adipogenesis. Both mouse and pig EBF2 could potently stimulate adipogenesis in unspecified multipotent mesodermal stem cells. However, in pigs, EBF2 expression was depleted during the critical lineage spec-ification time window, thus preventing the embryonic formation and development of porcine BAT. Hence, the elevation of EBF2 in mice, but not in pigs, promote chromatin activation to drive the progressive spec-ification of brown fat lineage. 2024 Published by Elsevier B.V. on behalf of Cairo University. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/)

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