数字农科院2.0

Fine-tuning rice heading date through multiplex editing of the regulatory regions of key genes by CRISPR-Cas9

文献类型： 外文期刊

作者： Shirong Zhou1; Liang Cai1;Haoqin Wu; Baoxiang Wang; Biao Gu; Song Cui; Xiaolong Huang;Zhuang Xu;Benyuan Hao;Haigang Hou; Yuan Hu; Chao Li;Yunlu Tian;Xi Liu;Liangming Chen;Shijia Liu; Ling Jiang; and Jianmin Wan;,*

期刊名称： Plant Biotechnology Journal

ISSN： 1467-7644

年卷期： 2023 年

页码：

收录情况： SCIE（2023版）

摘要： Heading date (or flowering time) is a key agronomic trait that affects seasonal and regional adaption of rice cultivars. An unoptimized heading date can either not achieve a high yield or has a high risk of encountering abiotic stresses. There is a strong demand on the mild to moderate adjusting the heading date in breeding practice. Genome editing is a promising method which allows more precise and faster changing the heading date of rice. However, direct knock out of major genes involved in regulating heading date will not always achieve a new germplasm with expected heading date. It is still challenging to quantitatively adjust the heading date of elite cultivars with best adaption for broader region. In this study, we used a CRISPR-Cas9 based genome editing strategy called high-efficiency multiplex promoter-targeting (HMP) to generate novel alleles at cis-regulatory regions of three major heading date genes: Hd1, Ghd7 and DTH8. We achieved a series of germplasm with quantitative variations of heading date by editing promoter regions and adjusting the expression levels of these genes. We performed field trials to screen for the best adapted lines for different regions. We successfully expanded an elite cultivar Ningjing8 (NJ8) to a higher latitude region by selecting a line with a mild early heading phenotype that escaped from cold stress and achieved high yield potential. Our study demonstrates that HMP is a powerful tool for quantitatively regulating rice heading date and expanding elite cultivars to broader regions.

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