数字农科院2.0

Photoperiod-regulated mitophagy in the germ cells of Brandt's voles (Lasiopodomys brandtii)

文献类型： 外文期刊

作者： Zhao, Lijuan;Chen, Chunxiao;Wang, Lewen;Liu, Yan;Gong, Fanglei;Wang, Jingou;Sun, Hong;Wang, Dawei;Wang, Zhenlong

作者机构：

关键词： Brandt's vole;mitophagy;photoperiod;spermatogenesis

期刊名称： INTEGRATIVE ZOOLOGY

ISSN： 1749-4877

年卷期： 2024 年

页码：

收录情况： SCIE（2024版） ; ; CSCD（2023-2024年度）

摘要： Photoperiod is a pivotal factor in affecting testicular function and spermatogenesis in seasonal-breeding animals. Mitophagy is essential for spermatogenesis, but its association with seasonal photoperiods has not been studied extensively. To explore this, we exposed male Brandt's voles (Lasiopodomys brandtii) to long-photoperiod (LP, 16 h/day) and short-photoperiod (SP, 8 h/day) conditions from their embryonic stages. Our results indicated that testis weight, volume, and relative testes weight were all significantly increased in LP compared to SP. Additionally, blood testosterone levels were markedly higher in LP than SP. Histological examination revealed that seminiferous diameter and epithelium thickness were greater in LP, with an increased abundance of germ cell types and cell numbers compared to SP. RT-qPCR analysis showed that mitophagy-promoting genes, such as Pink1, Prkn, Tomm7, Mnf2, Lc3, Optn, Gabarap, and Nbr1 were all upregulated in LP. Fluorescence in situ hybridization indicated that Pink1 expression was present in spermatogonia in SP, while in LP, Pink1 expression extended to almost all germ cell types with significantly higher mean optical density. Prkn expression was found in all germ cell types in both LP and SP, with a significantly higher mean optical density of 10-week-old LP males. Transmission electron microscopy showed normal mitochondrial morphology with clear membranes in SP, while the LP group had reduced cristae in mitochondria and damaged mitochondria undergoing autophagy. This study suggests that mitophagy may be involved in the photoperiodic spermatogenesis in Brandt's voles, providing insights into the role of photoperiod in seasonal reproduction in wild animals.

分类号：

  • 相关文献

[1]Dynamic Expressions Of Hypothalamic Genes R.egulate Seasonal Breeding In A Natural Rodent Population. Wang, DW,Li, N,Tian, L,Ren, F,Li, ZG,Chen, Y,Liu, L,Hu, XF,Zhang, XC,Song, Y,Hut, RA,Liu, XH. 2019

[2]Transcriptome analysis of the hypothalamus and testes in Brandt's Vole: new insights into mechanisms of photoperiodic plasticity in postnatal testicular development. Wang, Lewen,Ying, Yaqi,Li, Ning,Song, Ying,Zhao, Lijuan,Sun, Hong,Wang, Zhenlong,Liu, Xiao-Hui,Wang, Dawei. 2025

[3]Retrotransposon involves in photoperiodic spermatogenesis in Brandt's voles (Lasiopodomys brandtii) by co-transcription with flagellar genes. Lijuan Zhao,Fanglei Gong,Kang Lou,Lewen Wang,Jingou Wang,Hong Sun,Dawei Wang,Yuhua Shi,Zhenlong Wang. 2024

[4]Kinship Analysis Reveals Reproductive Success S.kewed Toward Overwintered Brandt'S V oles In Semi-Natural Enclosures. Chen, Y,Wang, DW,Li, N,Hu, XF,Ren, F,Hao, WL,Song, Y,Liu, XH. 2019

[5]Short photoperiod inhibited gonadal growth and elevated hypothalamic Dio3 expression unrelated to promoter DNA methylation in young Brandt's voles. Qiao, Yanting,Li, Ning,Song, Ying,Liu, Xiaohui,Wang, Dawei. 2024

[6]Role of Mitophagy in Regulating Intestinal Oxidative Damage. Xiaobin Wen,Lixin Tang,Ruqing Zhong,Lei Liu,Liang Chen,Hongfu Zhang. 2023

[7]Newcastle disease virus degrades SIRT3 via PINK1-PRKN-dependent mitophagy to reprogram energy metabolism in infected cells. Gong, Yabin,Tang, Ning,Liu, Panrao,Sun, Yingjie,Lu, Shanxin,Liu, Weiwei,Tan, Lei,Song, Cuiping,Qiu, Xusheng,Liao, Ying,Yu, Shengqing,Liu, Xiufan,Lin, Shu-Hai,Ding, Chan. 2021

[8]MoWhi2 Mediates Mitophagy to Regulate Conidiation and Pathogenesis in Magnaporthe oryzae. Shuai Meng,Jane Sadhna Jagernath,Chaoxi Luo,Huanbin Shi,Yanjun Kou. 2022

[9]The nucleoprotein of influenza A virus inhibits the innate immune response by inducing mitophagy. Zhang, Bo,Xu, Shuai,Liu, Minxuan,Wei, Yanli,Wang, Qian,Shen, Wentao,Lei, Cao-Qi,Zhu, Qiyun. 2022

[10]NDV-induced autophagy enhances inflammation through NLRP3/Caspase-1 inflammasomes and the p38/MAPK pathway. Juncheng Cai,Siyuan Wang,Haoyun Du,Lei Fan,Wei Feng Yuan,Qiufan Xu,Jinlian Ren,Qiuyan Lin,Bin Xiang,Chan Ding,Tao Ren,Libin Chen. 2023

[11]The role of selective autophagy in pathogen infection. Tang, Li,Song, Yinjuan,Xu, Jian,Chu, Yuefeng. 2023

[12]The PI3K/Akt-Nrf2 Signaling Pathway and Mitophagy Synergistically Mediate Hydroxytyrosol to Alleviate Intestinal Oxidative Damage. Wen, Xiaobin,Tang, Shanlong,Wan, Fan,Zhong, Ruqing,Chen, Liang,Zhang, Hongfu. 2024

[13]Salmonella Typhimurium persistently infects host via its effector SseJ-induced PHB2-mediated mitophagy. Sun, Dage,Gou, Hongchao,Zhang, Yu,Li, Jiayi,Dai, Changzhi,Shen, Haiyan,Chen, Kaifeng,Wang, Yu,Pan, Peng,Zhu, Ting,Xu, Chenggang,Shan, Tongling,Liao, Ming,Zhang, Jianmin. 2025

[14]CaEch1-mediated mitophagy regulates vegetative growth, conidiation, appressorium formation, and pathogenicity in Colletotrichum camelliae. Meng, Shuai,Xiong, Meng,Cheng, Longjun,Wang, Li,Chen, Ya,Luo, Chaoxi,Chao, Shufen. 2025

[15]Pseudorabies virus infection triggers mitophagy to dampen the interferon response and promote viral replication. Zhao, Yuan,Ding, Chan,Zhu, Zhenbang,Wang, Wenqiang,Wen, Wei,Favoreel, Herman W.,Li, Xiangdong. 2024

[16]Heat shock protein 60 manipulates Foot-and-Mouth disease virus replication by regulating mitophagy. Tang, Jianli,Abdullah, Sahibzada Waheed,Sun, Shiqi,Guo, Huichen. 2025

[17]BdUth1 Mediates Mitophagy by Recruiting BdAtg8 to Govern the Pathogenicity of Botryosphaeria dothidea. Meng, Shuai,Xiong, Meng,Sun, Yu,Xiong, Xiong,Cheng, Longjun,Luo, Chaoxi,Wang, Li,Chao, Shufen. 2025

[18]The effects of IGF-1 on mouse spermatogenesis using an organ culture method. Yao, Jianfei,Gao, Jun,Wang, Mingming,Li, Xiangdong,Zuo, Haiyang,Wang, Dong.

[19]DNA methylation-mediated transcription factors regulate Piwil1 expression during chicken spermatogenesis. Qiu, Lingling,Xu, Lu,Chang, Guobin,Gu, Qixin,Bi, Yulin,Zhang, Yu,Lu, Wei,Ren, Lichen,Zhu, Pengfei,Mu, Yun,Zhang, Yang,Xu, Qi,Chen, Guohong,Liu, Xiangping,Wang, Hongzhi,Wang, Kehua.

[20]Regulation of steroid hormones and energy status with cysteamine and its effect on spermatogenesis. Wang, Yandi,Yu, Shuai,Feng, Yandi,Chu, Meigiang,Cui, Liantao,Li, Lan,Zhang, Pengfei,Shen, Wei,Min, Lingjiang,Wang, Yandi,Zhao, Yong,Yu, Shuai,Feng, Yandi,Chu, Meigiang,Cui, Liantao,Li, Lan,Shen, Wei,Min, Lingjiang,Zhao, Yong,Zhang, Hongfu,Kou, Xin.

作者其他论文 更多>>