数字农科院2.0

Appropriate reduction of importin-α gene expression enhances yellow dwarf disease resistance in common wheat

文献类型： 外文期刊

作者： Wang, Lina;Zhang, Kunpu;Wang, Zhaohui;Yang, Jin;Kang, Guozhang;Liu, Yan;You, Liyuan;Wang, Xifeng;Jin, Huaibing;Wang, Daowen;Guo, Tiancai

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关键词： Barley yellow dwarf virus;disease resistance;genome editing;importin;nuclear transport;wheat

期刊名称： PLANT BIOTECHNOLOGY JOURNAL

ISSN： 1467-7644

年卷期： 2023 年

页码：

收录情况： SCIE（2023版）

摘要： Barley yellow dwarf viruses (BYDVs) cause widespread damage to global cereal crops. Here we report a novel strategy for elevating resistance to BYDV infection. The 17K protein, a potent virulence factor conserved in BYDVs, interacted with barley IMP-alpha 1 and -alpha 2 proteins that are nuclear transport receptors. Consistently, a nuclear localization signal was predicted in 17K, which was found essential for 17K to be transported into the nucleus and to interact with IMP-alpha 1 and -alpha 2. Reducing HvIMP-alpha 1 and -alpha 2 expression by gene silencing attenuated BYDV-elicited dwarfism, accompanied by a lowered nuclear accumulation of 17K. Among the eight common wheat CRISPR mutants with two to four TaIMP-alpha 1 and -alpha 2 genes mutated, the triple mutant alpha 1(aaBBDD)/alpha 2(AAbbdd) and the tetra-mutant alpha 1(aabbdd)/alpha 2(AAbbDD) displayed strong BYDV resistance without negative effects on plant growth under field conditions. The BYDV resistance exhibited by alpha 1(aaBBDD)/alpha 2(AAbbdd) and alpha 1(aabbdd)/alpha 2(AAbbDD) was correlated with decreased nuclear accumulation of 17K and lowered viral proliferation in infected plants. Our work uncovers the function of host IMP-alpha proteins in BYDV pathogenesis and generates the germplasm valuable for breeding BYDV-resistant wheat. Appropriate reduction of IMP-alpha gene expression may be broadly useful for enhancing antiviral resistance in agricultural crops and other economically important organisms.

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