数字农科院2.0

Monitoring Xylem Transport in the Stem of Lilium lancifolium Using Fluorescent Dye 5(6)-Carboxyfluorescein Diacetate

文献类型： 外文期刊

作者： Luo, Yulin;Yang, Panpan;Bi, Mengmeng;Xu, Leifeng;Du, Fang;Ming, Jun

作者机构：

关键词： Lilium;xylem transport;stem;fluorescent dye;CFDA

期刊名称： PHYTON-INTERNATIONAL JOURNAL OF EXPERIMENTAL BOTANY

ISSN： 0031-9457

年卷期： 2024 年

页码：

收录情况： SCIE（2024版）

摘要： The xylem undergoes physiological changes in response to various environmental conditions during the process of plant growth. To understand these physiological changes, it is extremely important to observe the transport of xylem. In this study, the distribution and structure of vascular bundle in Lilium lancifolium were observed using the method of semithin section. Methods for introducing a fluorescent tracer into the xylem of the stems were evaluated. Then, the transport rule of 5(6)-Carboxyfluorescein diacetate (CFDA) in the xylem of the stem of L. lancifolium was studied by fluorescence dye in live cells tracer technology. The results showed that the vascular bundles of L. lancifolium were scattered in the basic tissue, the peripheral vascular bundles were smaller and densely distributed, and the closer to the center, the larger the volume of vascular bundles and the more sparsely distributed. The vascular bundles of L. lancifolium are limited external tenacity vascular bundles, which are composed of phloem and xylem. The most suitable method for CFDA labeling the xylem of isolated stem segments of L. lancifolium was solution soaking for 24 h. The running speed of CF in the isolated stem was 0.3 cm/h, which was consistent with the running speed of the material in the field. CF could be transported between the xylem and parenchyma cells, indicating that the material transport in the xylem could be through the symplastic pathway. The above results laid a foundation for the study of the xylem transport mechanism and the xylem pathogen disease of lily.

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