数字农科院2.0

Exogenous abscisic acid represses rice flowering via SAPK8-ABF1- Ehd1 / Ehd2 pathway

文献类型： 外文期刊

作者： Tang, Liqun;Li, Guanghao;Wang, Huimei;Zhao, Juan;Li, Zhiyong;Liu, Xixi;Shu, Yazhou;Liu, Wanning;Wang, Shuang;Huang, Jie;Ying, Jiezheng;Tong, Xiaohong;Yuan, Wenya;Wei, Xiangjin;Tang, Shaoqing;Wang, Yifeng;Bu, Qingyun;Zhang, Jian

作者机构：

关键词： Abscisic acid;bZIP;Flowering;Polycomb Repressive Complex 2 (PRC2);Rice(Oryza sativa L.);SnRK2

期刊名称： JOURNAL OF ADVANCED RESEARCH

ISSN： 2090-1232

年卷期： 2024 年 59 卷

页码：

收录情况： SCIE（2024版）

摘要： Introduction: Rice flowering is a major agronomic trait, determining yield and ecological adaptability in particular regions. ABA plays an essential role in rice flowering, but the underlying molecular mechanism remains largely elusive. Objectives: In this study, we demonstrated a SAPK8-ABF1-Ehd1/Ehd2 pathway, through which exogenous ABA represses rice flowering in a photoperiod-independent manner. Methods: We generated abf1 and sapk8 mutants using the CRISPR-Cas9 method. Using yeast two-hybrid, Pull down, BiFC and kinase assays, SAPK8 interacted and phosphorylated ABF1. ABF1 directly bound to the promoters of Ehd1 and Ehd2 using ChIP-qPCR, EMSA, and LUC transient transcriptional activity assay, and suppressed the transcription of these genes. Results: Under both long day and short day conditions, simultaneous knock-out of ABF1 and its homolog bZIP40 accelerated flowering, while SAPK8 and ABF1 over-expression lines exhibited delayed flowering and hypersensitivity to ABA-mediated flowering repression. After perceiving the ABA signal, SAPK8 physically binds to and phosphorylates ABF1 to enhance its binding to the promoters of master positive flowering regulators Ehd1 and Ehd2. Upon interacting with FIE2, ABF1 recruited PRC2 complex to deposit H3K27me3 suppressive histone modification on Ehd1 and Ehd2 to suppress these genes transcription, thereby leading flowering. Conclusion: Our work highlighted the biological functions of SAPK8 and ABF1 in ABA signaling, flowering control and the involvement of a PRC2-mediated epigenetic repression mechanism in the transcription regulation governed by ABF1 on ABA-mediated rice flowering repression. (c) 2024 The Authors. Published by Elsevier B.V. on behalf of Cairo University. This is an open access article under the CC BY -NC -ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

分类号：

  • 相关文献

[1]植物多功能调控因子SnRK2研究进展. 苗丽丽,刘秀林,张宏纪,毛新国,景蕊莲. 2019

[2]两个新的黄瓜SnRK2基因的鉴定及特征分析. 曹齐卫,周建贵,刘明毓,王永强,杨桂兰,孙小镭,李利斌. 2015

[3]柑橘响应溃疡病菌转录因子CsBZIP40的克隆及功能分析. 贾瑞瑞,周鹏飞,白晓晶,陈善春,许兰珍,彭爱红,雷天刚,姚利晓,陈敏,何永睿,李强. 2017

[4]拟南芥花青素合成途径重要转录因子的研究进展. 洪雪,张少斌,徐江,崔月. 2014

[5]GST pull-down联合LC-MS/MS筛选柑橘抗溃疡病转录因子CsBZIP40的互作蛋白. 窦万福,祁静静,胡安华,陈善春,彭爱红,许兰珍,雷天刚,姚利晓,何永睿,李强. 2019

[6]bZIP转录因子与植物抗逆性研究进展. 曹红利,岳川,王新超,杨亚军. 2012

[7]A cluster of mutagenesis revealed an osmotic regulatory role of the OsPIP1 genes in enhancing rice salt tolerance. Tao, Leyuan,Wang, Bing,Xin, Shichao,Li, Wei,Huang, Shengcai,Liu, Laihua,Cui, Jing,Zhang, Qianru,Cheng, Xianguo. 2023

[8]Overexpression of a maize SNF-related protein kinase gene, ZmSnRK2.11, reduces salt and drought tolerance in Arabidopsis. Zhang Fan,Wang Jian-Hua,Zhang Fan,Chen Xun-Ji,Zheng Jun,Chen Xun-Ji. 2015

[9]Overexpression of sugarcane gene SoSnRK2.1 confers drought tolerance in transgenic tobacco. Sun, Bo,Niu, Jun-Qi,Tan, Qin-Liang,Li, Jian,Yang, Li-Tao,Li, Yang-Rui,Yang, Li-Tao,Li, Yang-Rui.

[10]The Modulation of Sucrose Nonfermenting 1-Related Protein Kinase 2.6 State by Persulfidation and Phosphorylation: Insights from Molecular Dynamics Simulations. Li, Miaomiao,Wu, Ting,Wang, Shuhan,Duan, Tianqi,Huang, Siqi,Xie, Yanjie. 2023

[11]Sucrose non-fermenting1-related protein kinase VcSnRK2.3 promotes anthocyanin biosynthesis in association with VcMYB1 in blueberry. Wang, Xuan,Tang, Qi,Chi, Fumei,Liu, Hongdi,Zhang, Hongjun,Song, Yang. 2023

[12]Improving pre-harvest sprouting resistance in rice by editing OsABA8ox using CRISPR/Cas9. Fu K., Song W., Chen C., Mou C., Huang Y., Zhang F., Hao Q., Wang P., Ma T., Chen Y., Zhu Z., Zhang M., Tong Q., Liu X., Jiang L., Wan J.. 2022

[13]The OsABF1 transcription factor improves drought tolerance by activating the transcription of COR413-TM1 in rice. Zhang, Chunyu,Li, Cong,Liu, Jun,Yu, Chunsheng,Li, Hongyu,Zhao, Tao,Liu, Bin,Lv, Yandong.

[14]ABP9, a maize bZIP transcription factor, enhances tolerance to salt and drought in transgenic cotton. Wang, Chunling,Lu, Guoqing,Hao, Yuqiong,Guo, Huiming,Zhao, Jun,Cheng, Hongmei,Wang, Chunling,Guo, Yan.

[15]The ethylene response factor AtERF11 that is transcriptionally modulated by the bZIP transcription factor HY5 is a crucial repressor for ethylene biosynthesis in Arabidopsis. Li, Zhuofu,Zhang, Lixia,Yu, Yanwen,Quan, Ruidang,Zhang, Zhijin,Zhang, Haiwen,Huang, Rongfeng,Li, Zhuofu,Zhang, Lixia,Yu, Yanwen,Quan, Ruidang,Zhang, Zhijin,Zhang, Haiwen,Huang, Rongfeng.

[16]The Osabf1 Transcription Factor Improves D.rought Tolerance By Activating T he Transcription Of Cor413-Tm1 In Rice. Zhang, CY, Li, C, Liu, J, Lv, YD, Yu, CS, Li, HY, Zhao, T, Liu, B. 2017

[17]Roles of the bZIP gene family in rice. Z.G. E , Y.P. Zhang , J.H. Zhou , L. Wang *. 2014

[18]Osabf1 represses gibberellin biosynthesis to regulate plant height and seed germination in rice (Oryza sativa l.). Liqun Tang,Huayu Xu,Yifeng Wang,Huimei Wang,Zhiyong Li,Xixi Liu,Yazhou Shu,Guan Li,Wanning Liu,Jiezheng Ying,Xiaohong Tong,Jialing Yao,Wenfei Xiao,Shaoqing Tang,Shen Ni,Jian Zhang. 2021

[19]Anthocyanin Accumulation and Molecular Analysis of Correlated Genes by Metabolomics and Transcriptomics in Sister Line Apple Cultivars. Shi, Caiyun,Liu, Li,Wei, Zhifeng,Liu, Junwei,Li, Ming,Yan, Zhenli,Gao, Dengtao. 2022

[20]Identification and Analysis of bZIP Family Genes in Potato and Their Potential Roles in Stress Responses. Qi Wang,Cun Guo,Zhiyuan Li,Jinhao Sun,Dong Wang,Liangtao Xu,Xiaoxu Li,Yongfeng Guo. 2021

作者其他论文 更多>>