数字农科院2.0

Characterization of a novel thermostable α-L-arabinofuranosidase for improved synergistic effect with xylanase on lignocellulosic biomass hydrolysis without prior pretreatment

文献类型： 外文期刊

作者： Liu X.;Gao F.;Wang Y.;Zhang J.;Bai Y.;Zhang W.;Luo H.;Yao B.;Wang Y.;Tu T.

作者机构：

关键词： Glycoside hydrolase family 51;Lignocellulosic biomass;Pretreatment;Synergistic degradation;Thermostable enzymes;Xylanase;α-L-Arabinofuranosidase

期刊名称： Bioresource Technology

ISSN： 0960-8524

年卷期： 2024 年 394 卷

页码：

收录情况： SCIE（2024版） ; ; EI（2024版）

摘要： Utilizing thermostable enzymes in biomass conversion processes presents a promising approach to bypass pretreatment, garnering significant attention from the biorefinery industry. A novel discovered α-L-arabinofuranosidase, Abf4980, exhibits exceptional thermostability by maintaining full activity after 24 h of incubation at 70 °C. It effectively acts on polyarabinosides, cleaving α-1,2- and α-1,3-linked arabinofuranose side chains from water-soluble wheat arabinoxylan while releasing xylose. When synergistically combined with the thermostable bifunctional xylanase/β-glucanase CbXyn10C from Caldicellulosiruptor bescii at an enzyme-activity ratio of 6:1, Abf4980 achieves the highest degradation efficiency for wheat arabinoxylan. Furthermore, Abf4980 and CbXyn10C demonstrated remarkable efficacy in hydrolyzing unmodified wheat bran and corn cob to generate arabinose and xylooligosaccharides. This discovery holds promising opportunities for improving the efficiency of lignocellulosic biomass conversion into fermentable sugars. © 2023 Elsevier Ltd

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