数字农科院2.0

Liquid Y2H-Seq, a Rapid and Data-Rich Alternative to Conventional Yeast Two-Hybrid Screening

文献类型： 外文期刊

作者： Qi, Yanping;Zhang, Chunlei;Yuan, Shan;Xu, Xin;Sun, Shi;Zhang, Lixin;Liu, Luping;Wu, Tingting;Han, Tianfu;Wu, Cunxiang;Jiang, Bingjun

作者机构：

关键词： GmFT1a;liquid Y2H-Seq;protein-protein interaction

期刊名称： PROTEOMICS

ISSN： 1615-9853

年卷期： 2025 年

页码：

收录情况： SCIE（2025版）

摘要： Highly laborious plating of cultures onto solid media is inevitable in conventional yeast two-hybrid systems. We have successfully developed Liquid Y2H-Seq, a method that replaces solid media with liquid media for culturing and displaces visual inspection of colonies with sequencing. Soybean (Glycine max) is a typical photoperiod-sensitive crop, meaning that a specific duration of light regime has a huge impact on soybean flowering and production. We obtained a lot of putative interactions for soybean flowering genes. Finally, these reports introduce a labor-reducing and time-saving method for identifying protein-protein interactions, based on simple modifications to a ubiquitous protocol in life science research.

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