数字农科院2.0

Critical Role of Transcription Factor CtSR in Mediating Ergosterol Biosynthesis and DMI Fungicides Sensitivity in Colletotrichum truncatum

文献类型： 外文期刊

作者： Sun, Wei;He, Yongqi;Yuan, Huizhu;Lei, Tengyu;Yan, Xiaojing;Yang, Daibin;Chen, Shuning

作者机构：

关键词： ergosterol biosynthesis;DMI fungicides;Colletotrichum spp.;transcription factor;fungicides resistance

期刊名称： JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY

ISSN： 0021-8561

年卷期： 2025 年

页码：

收录情况： SCIE（2025版） ; ; EI（2025版）

摘要： Anthracnose caused by Colletotrichum truncatum severely impacts global fruit/vegetable yields. Unlike other Colletotrichum species, C. truncatum exhibits inherent resistance to some sterol demethylation inhibitors (DMIs), necessitating an understanding of ergosterol synthesis regulation and resistance mechanisms. A transcription factor, CtSR (CTRU02_06681), that regulates the DMI sensitivity in C. truncatum was identified in our study. Deletion of CtSR rendered a naturally resistant isolate highly sensitive to DMIs, significantly reduced total ergosterol levels, decreased CYP51s expression compared to the wild type, and abolished DMI-induced expression of CYP51s. Deletion of CtSR reduced mycelial growth and virulence and made the strain highly sensitive to oxidative stress but did not affect its sensitivity to osmotic stress. Transcriptomic analysis and yeast one-hybrid assays confirmed that CtSR directly binds the conserved promoter motif CGAATACGAA to regulate ergosterol biosynthesis genes. Taken together, our study demonstrates a critical role for CtSR in the regulation of ergosterol biosynthesis and DMI interactions in C. truncatum, which may have significant practical implications.

分类号：

  • 相关文献

[1]Lamp Detection Of The Genetic E.lement 'Mona' Associated With D mi Resistance In Monilinia Fructicola. Chen, Shuning,Yuan, Huizhu,Schnabel, Guido,Luo, Chaoxi,Luo, Chaoxi. 2019

[2]Paralogous Cyp51 Genes Of Colletotrichum Spp. Mediate Differential Sensitivity To Sterol Demethylation Inhibitors. Chen, SN, Hu, MJ, Schnabel, G, Yang, DB, Yan, XJ, Yuan, HZ. 2020

[3]Protein-DNA interactions in the promoter region of the gene encoding diapause hormone and pheromone biosynthesis activating neuropeptide of the cotton bollworm, Helicoverpa armigera. Hong, B,Zhang, ZF,Tang, SM,Yi, YZ,Zhang, TY,Xu, WH.

[4]A wheat R2R3-MYB gene, TaMYB30-B, improves drought stress tolerance in transgenic Arabidopsis. Zhang, Lichao,Zhao, Guangyao,Xia, Chuan,Jia, Jizeng,Liu, Xu,Kong, Xiuying. 2012

[5]Jasmonate-responsive transcription factors regulating plant secondary metabolism. Zhou, Meiliang,Zhou, Meiliang,Memelink, Johan.

[6]Characteristic Expression Analysis of Five WRKY Transcriptional Factors in Rice Leaf Growth and Disease Resistance Reaction. Miao Liu-Yang,Li Li-Yun,Liu Zhao,Liu Yu-Meng,Liu Guo-Zhen,Jiang Guang-Huai,Yang Feng-Huan,He Chen-Yang. 2013

[7]Isolation and functional characterization of a R2R3-MYB regulator of the anthocyanin biosynthetic pathway from Epimedium sagittatum. Huang, Wenjun,Khaldun, A. B. M.,Lv, Haiyan,Du, Liuwen,Wang, Ying,Du, Liuwen,Zhang, Chanjuan.

[8]Identification and functional characterization of the NAC gene promoter from Populus euphratica. Wang, Jun-Ying,Wang, Jun-Ping,Yang, Hai-Feng.

[9]The ARID-HMG DNA-binding protein AtHMGB15 is required for pollen tube growth in Arabidopsis thaliana. Xia, Chuan,Wang, Yu-Jiao,Liang, Yan,Niu, Qian-Kun,Tan, Xiao-Yun,Chu, Liang-Cui,Chen, Li-Qun,Zhang, Xue-Qin,Ye, De,Xia, Chuan,Ye, De.

[10]A R2R3-MYB Transcription Factor Regulates the Flavonol Biosynthetic Pathway in a Traditional Chinese Medicinal Plant, Epimedium sagittatum. Huang, Wenjun,Khaidun, A. B. M.,Chen, Jianjun,Lv, Haiyan,Wang, Ying,Zhang, Chanjuan,Yuan, Ling. 2016

[11]THE ROLE OF R2R3MYB TRANSCRIPTION FACTORS IN PLANT STRESS TOLERANCE. Xie, R. J.,Deng, L.,He, S. L.,Yi, S. L.,Zheng, L.. 2014

[12]Differential expression of CYB5A in Chinese and European pig breeds due to genetic variations in the promoter region. Bai, Y.,Zhang, J. B.,Fang, M. Y.,Bai, Y.,Xue, Y.,Peng, Y. L.,Chen, G..

[13]The study of regulatory effects of Pdx-1, MafA and NeuroD1 on the activity of porcine insulin promoter and the expression of human islet amyloid polypeptide. Liu, Xiao-Dan,Ruan, Jin-Xue,Xia, Ji-Han,Yang, Shu-Lin,Fan, Jun-Hua,Li, Kui.

[14]Identification of a POU factor involved in regulating the neuron-specific expression of the gene encoding diapause hormone and pheromone biosynthesis-activating neuropeptide in Bombyx mori. Zhang, TY,Kang, L,Zhang, ZF,Xu, WH.

[15]Mutations of genes in synthesis of the carotenoid precursors of ABA lead to pre-harvest sprouting and photo-oxidation in rice. Fang, Jun,Chai, Chenglin,Li, Chunlai,Tang, Jiuyou,Huang, Zejun,Guo, Xiaoli,Sun, Changhui,Liu, Min,Wang, Yiqin,Cheng, Zhukuan,Chu, Chengcai,Fang, Jun,Chai, Chenglin,Li, Chunlai,Tang, Jiuyou,Huang, Zejun,Guo, Xiaoli,Sun, Changhui,Liu, Min,Wang, Yiqin,Cheng, Zhukuan,Chu, Chengcai,Fang, Jun,Chai, Chenglin,Li, Chunlai,Tang, Jiuyou,Huang, Zejun,Guo, Xiaoli,Qian, Qian,Sun, Lei,Zhang, Yan,Lu, Qingtao,Lu, Congming,Han, Bin,Chen, Fan.

[16]The 14-3-3 protein GENERAL REGULATORY FACTOR11 (GRF11) acts downstream of nitric oxide to regulate iron acquisition in Arabidopsis thaliana. Yang, Jian Li,Chen, Wei Wei,Chen, Li Qian,Zheng, Shao Jian,Chen, Wei Wei,Qin, Cheng,Jin, Chong Wei,Shi, Yuan Zhi.

[17]Characterization of Squamosa Promoter Binding Protein-LIKE Genes in Wheat. Wang, Bingnan,Geng, Shuaifeng,Wang, Di,Feng, Nan,Zhang, Dongdong,Wu, Liang,Hao, Chenyang,Zhang, Xueyong,Li, Aili,Mao, Long.

[18]Bioinformatic identification and expression analysis of the Malus domestica DREB2 transcription factors in different tissues and abiotic stress. Li, Huifeng,Sun, Xiaoli,Jia, Houzhen,Ran, Kun,Zhao, Qiang.

[19]Genome-wide analysis of the MADS-box gene family in Populus trichocarpa. Leseberg, Charles H.,Li, Aili,Kang, Hui,Duvall, Melvin,Mao, Long. 2006

[20]Characterization of the NLRC5 promoter in chicken: SNPs, regulatory elements and CpG islands. Guo, X. M.,Chang, G. B.,Xu, L.,Bi, Y. L.,Zhang, Y.,Zhu, P. F.,Wu, Y.,Chen, G. H.,Liu, X. P.,Wang, H. Z..

作者其他论文 更多>>