数字农科院2.0

HL-TRP channel is required for various repellents for the parthenogenetic Haemaphysalis longicornis

文献类型： 外文期刊

作者： Kuang, Ceyan;Cao, Jie;Zhou, Yongzhi;Zhang, Houshuang;Wang, Yanan;Zhou, Jinlin

作者机构：

关键词： HL-TRP channel;Icaridin;DEET;Cinnamaldehyde;Olfaction

期刊名称： PARASITES & VECTORS

ISSN： 1756-3305

年卷期： 2025 年 18 卷 1 期

页码：

收录情况： SCIE（2025版）

摘要： BackgroundTicks can transmit a wide range of pathogens that endanger human and animal health. Although repellents are commonly used for tick control, understanding their mechanisms aren't complete.MethodsThe repellent effects of N, N-diethyl-meta-toluamide (DEET); sec-butyl 2-(2-hydroxyethyl) piperidine-1-carboxylate (icaridin); N, N-diethyl-3-methylbenzamide (IR3535); and cinnamaldehyde on the parthenogenetic tick Haemaphysalis longicornis at the nymph stage were assessed using Y-tubes. The involvement of transient receptor potential (HL-TRP) channel molecules in the repellent mechanism was investigated through in situ hybridization, subcellular localization, real-time fluorescence quantitative polymerase chain reaction (PCR), RNA interference, and electroantennography. In addition, the binding affinity of HL-TRP molecules to repellents was predicted using AlphaFold3.ResultsDEET, icaridin, IR3535, and cinnamaldehyde have been shown to effectively repel nymphs. HL-TRP channel is shared among various arthropods, particularly several species of ticks. It is localized to the cell membrane and Haller's organ. Moreover, microinjection of double-stranded RNA elicited tick repellency behavior, and the electroantennogram responses to those repellents were significantly decreased. The TYR783 site was proposed as an essential binding site to establish hydrogen bonds with icaridin, DEET, and cinnamaldehyde.ConclusionsThis exploration of ticks and repellents found that HL-TRP channel functions as a chemosensory receptor for repellents and, thereby, mediates avoidance behavior.

分类号：

  • 相关文献

[1]In vitro anti-aflatoxigenic effect and mode of action of cinnamaldehyde against aflatoxin B-1. Sun, Qi,Liu, Yang,Wang, Ling,Lu, Zhisong.

[2]Growth inhibition and morphological alterations of Fusarium verticillioides by cinnamon oil and cinnamaldehyde. Xing, Fuguo,Hua, Huijuan,Selvaraj, Jonathan Nimal,Zhao, Yueju,Zhou, Lu,Liu, Xiao,Liu, Yang.

[3]Effects of wheat dried distillers' grains with solubles and cinnamaldehyde on in vitro fermentation and protein degradation using the Rusitec technique. Li, Yangling,He, Maolong,Li, Chun,Forster, Robert,Beauchemin, Karen Anne,Yang, Wenzhu,Li, Yangling,Li, Chun.

[4]Cinnamaldehyde inhibits fungal growth and aflatoxin B-1 biosynthesis by modulating the oxidative stress response of Aspergillus flavus. Sun, Qi,Shang, Bo,Liu, Yang,Wang, Ling,Lu, Zhisong.

[5]Microencapsulation enhances the antifungal activity of cinnamaldehyde during the period of peanut storage. Chenxi Zhang,Lin Pan,Junning Ma,Qiang Yu,Xiaohua Yu,Sarfaraz Hussain,Xu Li,Yujie Li,Yue Guan,Yifei Li,Fuguo Xing. 2023

[6]Inhibition of Essential Oils on Growth of Aspergillus flavus and Aflatoxin B1 Production in Broth and Poultry Feed. Han B.,Fu G.-W.,Wang J.-Q.. 2022

[7]Cinnamaldehyde affects lipid droplets metabolism after adipogenic differentiation of C2C12 cells. Liu, Yanrong,Liu, Zhengqun,Luo, Qiang,Sun, Zhuwen,Li, Ning,Zheng, Zi,Mu, Shuqin,Zhou, Xiaoqiao,Yan, Jun,Sun, Chao,Zhang, Hongfu. 2022

[8]Dual-conjugation of aminated sugar beet pectin-oxidized tannic acid nanonetworks with cinnamaldehyde at the oil-water interface to stabilize a 3D printable high internal phase emulsion. Hualei Chen,Shujuan Yu,Fei Pan,Xiangxin Li,Xiaoming Fang,Wenjun Peng,Wenli Tian. 2025

[9]Construction of cellulose nanocrystals based rod-like electrostatic nanocomplexes and the digestion characteristics of its stable high internal phase emulsions in vitro. Hualei Chen,Fei Pan,Wenjun Peng,Xiangxin Li,Xiaoming Fang,Wenli Tian. 2025

[10]Identification of chemosensory protein inhibitors as a potential strategy to combat pyrethroid resistance in Anopheles gambiae. Liu, Chen,Sundarraj, Rajamanikandan,Yang, Ning,Dong, Meijing,Yuchi, Zhiguang. 2025

[11]Olfactory co-receptor identification and expression pattern analysis in polyembryonic endoparasitoid Macrocentrus cingulum. Ahmed, Tofael,Zhang, Tian-tao,Wang, Zhen-ying,He, Kang-lai,Bai, Shu-xiong,Ahmed, Tofael. 2015

[12]Proteomic analysis of castor bean tick Ixodes ricinus: a focus on chemosensory organs. Dani, Francesca Romana,Ban, Liping,Song, Limei,Pelosi, Paolo,Dani, Francesca Romana.

[13]EXPRESSION OF SNMP1 AND SNMP2 GENES IN ANTENNAL SENSILLA OF Spodoptera exigua (HUBNER). Liu, Chengcheng,Zhang, Jin,Dong, Shuanglin,Liu, Chengcheng,Zhang, Jin,Liu, Yang,Wang, Guirong.

[14]Identification and expression profile analysis of odorant-binding protein genes in Apolygus lucorum (Hemiptera: Miridae). Gu, Shao-Hua,Liu, Jing-Tao,Zhu, Xiao-Qiang,Guo, Yu-Yuan,Zhang, Yong-Jun,Liu, Jing-Tao,Zhou, Jing-Jiang.

[15]Western honeybee drones and workers (Apis mellifera ligustica) have different olfactory mechanisms than eastern honeybees (Apis cerana cerana). Woltedji, Dereje,Song, Feifei,Zhang, Lan,Gala, Alemayehu,Han, Bin,Feng, Mao,Fang, Yu,Li, Jianke.

[16]Fluorescence competition assay for the assessment of green leaf volatiles and trans-beta-farnesene bound to three odorant-binding proteins in the wheat aphid Sitobion avenae (Fabricius). Yin, Jiao,Deng, Sisi,Li, Kebin,Cao, Yazhong.

[17]Male-Produced Aggregation Pheromone of Coffee Bean Weevil, Araecerus fasciculatus. Zhang, Xiao-Fang,Chen, Dan,Zhang, Tao,Yang, Shuai,Gao, Yu-Lin,She, Dong-Mei,Ning, Jun.

[18]Expression and functional analysis of Niemann-Pick C2 gene in Phytoseiulus persimilis. Zhou, Hongxu,Yan, Hong,Wang, Endong,Zhang, Bo,Xu, Xuenong. 2023

[19]The larvae of Phyllotreta striolata share the same olfactory cues for locating Brassicaceae plant with conspecific adults. Xiao, Yong,Sun, Liang,Wu, Yuhong,Wang, Qian,Zhang, Yongjun,Jing, Xiangfeng,Li, Zhenyu. 2023

[20]Identification and Functional Analysis of Odorant Binding Proteins in Apriona germari (Hope). Yuan, Tingting,Mang, Dingze,Purba, Endang R.,Ye, Jia,Qian, Jiali,Rao, Fuqiang,Wang, Haichao,Wu, Zhenchen,Zhang, Wenjing,Zheng, Yongxin,Zhang, Qing-He,Li, Zhaoqun,Zhang, Longwa. 2024

作者其他论文 更多>>