数字农科院2.0

Epitope Mapping of Senecavirus A 3A Protein Using Monoclonal Antibodies

文献类型： 外文期刊

作者： Meng, Liang;Tian, Xiao-Xiao;Xiang, Xu-Yan;Qi, Xin-Yu;Zhou, Han-Rong;Xiao, Pei-Yu;An, Tong-Qing;Meng, Fan-Dan;Wang, Hai-Wei

作者机构：

关键词： 3A protein;epitope;monoclonal antibody;Senecavirus A

期刊名称： TRANSBOUNDARY AND EMERGING DISEASES

ISSN： 1865-1674

年卷期： 2025 年 2025 卷 1 期

页码：

收录情况： SCIE（2025版）

摘要： Senecavirus A (SVA), an emerging pathogen causing vesicular disease in pigs, poses a significant threat to the swine industry. The nonstructural protein 3A of SVA plays an essential role in the viral replication cycle. In this study, we immunized mice with the prepared SVA 3A protein and produced two monoclonal antibodies (mAbs), AG4 and 2F3. MAb AG4 showed specific reactivity to the linear and conformational 3A protein, whereas mAb 2F3 did not recognize linear epitope of 3A protein. Through truncated 3A protein expression and alanine mutation analysis, we identified 1SPNEND6 as the minimal motif recognized by mAb AG4, with Asn3 being the critical residue. Additionally, we demonstrated that mAb 2F3 failed to recognize the SVA mutant with the 75QEETEG80 deletion in 3A protein, indicating that 75QEETEG80 constitutes an essential epitope for mAb 2F3. Further deletion analysis confirmed that 75QE76 is the crucial motif for mAb 2F3 recognition. Moreover, we found that 1SPNEND6 and 75QEETEG80 are highly conserved among different SVA strains and are exposed on the surface of the 3A protein. This study contributes to further explore the function of SVA 3A protein and develop diagnostic tools for SVA detection.

分类号：

  • 相关文献

[1]Development of an indirect ELISA using a novel linear epitope at the C-terminal region of the VP2 protein to specifically detect antibodies against Senecavirus A. Zhongyuan Ma,Jianliang Lv,Zhongwang Zhang,Li Pan. 2022

[2]Development and Evaluation of a Competitive Enzyme-Linked Immunosorbent Assay Based on Swine Monoclonal Antibodies for Detecting Neutralizing Antibodies against Senecavirus A. Ma, Xueqing,Huang, Jiaxin,Li, Kun,Ding, Kailu,Fu, Yuanfang,Zhang, Jing,Zhao, Zhixun,Li, Pinghua,Bai, Xingwen,Li, Dong,Liu, Xia,Zeng, Qiaoying,Liu, Zaixin,Sun, Pu,Lu, Zengjun. 2023

[3]Identification of a linear B-cell epitope within the Bluetongue virus serotype 8 NS2 protein using a phage-displayed random peptide library. Qin, Yong-Li,Sun, En-Cheng,Liu, Ni-Hong,Yang, Tao,Xu, Qing-Yuan,Zhao, Jing,Wang, Wen-Shi,Wei, Peng,Feng, Yu-Fei,Li, Jun-Ping,Wu, Dong-Lai,Qin, Yong-Li,Sun, En-Cheng,Zhao, Jing,Wang, Wen-Shi,Wei, Peng,Li, Jun-Ping. 2013

[4]Fine level epitope mapping and conservation analysis of two novel linear B-cell epitopes of the avian infectious bronchitis coronavirus nucleocapsid protein. Han, Zongxi,Zhao, Fei,Shao, Yuhao,Liu, Xiaoli,Kong, Xiangang,Song, Yang,Liu, Shengwang.

[5]Identification of a linear epitope on the capsid protein of classical swine fever virus. Zhang, Xin,Sun, Yuan,Li, Su,Li, Na,Yang, Shuo,He, Fan,Huang, Jun-Hua,Ling, Li-Jun,Qiu, Hua-Ji,Xu, Jia. 2011

[6]Production and characterization of mouse monoclonal antibodies against lysis protein E of phiX174. Yu, Shenye,Zhao, Hailing,Wang, Huanan,Wang, Xiumei,Xu, Lili,Si, Wei,Chen, Liping,Zhang, Wanjiang,Liu, Siguo,Shao, Guangxi. 2013

[7]Identification of two antigenic epitopes on SARS-CoV spike protein. Hua, RH,Zhou, YJ,Wang, YF,Hua, YZ,Tong, GZ. 2004

[8]Identification of a conserved linear epitope on the VP1 protein of serotype O foot-and-mouth disease virus by neutralising monoclonal antibody 8E8. Yang, Decheng,Zhang, Chunyuan,Zhao, Lei,Zhou, Guohui,Wang, Haiwei,Yu, Li. 2011

[9]Identification of monoclonal antibody targeting epitope on p72 trimeric spike of African swine fever virus. Duan, Xulai,Liu, Yingnan,Chen, Zongyan,Xie, Zhenhua,Tian, Chuanwen,Li, Yao,Lv, Lu,Wang, Rongrong,Liu, Jingyi,Chen, Hongjun. 2023

[10]Identification of one novel epitope targeting p54 protein of African swine fever virus using monoclonal antibody and development of a capable ELISA. Yunlei Cao,Dongmei Han,Yujiao Zhang,Kuan Zhang,Nannan Du,Wu Tong,Guoxin Li,Hao Zheng,Changlong Liu,Fei Gao,Guangzhi Tong. 2021

[11]Expression of ASFV p17 in CHO cells and identification of one novel epitope using a monoclonal antibody. Liwei Li,Sina Qiao,Shumao Wang,Jiachen Liu,Kuan Zhao,Yanjun Zhou,Guoxin Li,Yifeng Jiang,Changlong Liu,Guangzhi Tong,Wu Tong,Fei Gao. 2023

[12]Identification of Two Linear Epitopes on MGF_110-13L Protein of African Swine Fever Virus with Monoclonal Antibodies. Shu Jian Zhang,Bei Niu,Shi Meng Liu,Yuan Mao Zhu,Dong Ming Zhao,Zhi Gao Bu,Rong Hong Hua. 2024

[13]Emergence and whole-genome sequence of Senecavirus A in Colombia. Sun, D.,Sun, D.,Vannucci, F.,Knutson, T. P.,Marthaler, D. G.,Corzo, C..

[14]Identification of B-cell epitopes on structural proteins VP1 and VP2 of Senecavirus A and development of a multi-epitope recombinant protein vaccine. Zhongwang Zhang,Fei Yao,Jianliang Lv,Yaozhong Ding,Xinsheng Liu,Liping Zhang,Zhongyuan Ma,Peng Zhou,Yonglu Wang,Huichen Guo,Li Pan. 2023

[15]A novel rapid detection of Senecavirus A using recombinase polymerase amplification (RPA) coupled with lateral flow (LF) dipstrip. Huibao Wang,Jinjie Dong,Tao Zhang,Fan Wang,Rui Yang,Yong Zhang,Xingxu Zhao. 2022

[16]Evaluation of antibody response in sows after vaccination with senecavirus a vaccine and the effect of maternal antibody transfer on antibody dynamics in offspring. Fan Yang,Zixiang Zhu,Huanan Liu,Weijun Cao,Wei Zhang,Ting Wei,Min Zheng,Keshan Zhang,Hong Tian,Qiaoying Zeng,Xuepeng Cai,Haixue Zheng. 2021

[17]Senecavirus A 2B protein suppresses type I interferon production by inducing the degradation of MAVS. Huanan Liu,Kangli Li,Wenzhe Chen,Fan Yang,Weijun Cao,Keshan Zhang,Pengfei Li,Lijie Tang,Zixiang Zhu,Haixue Zheng. 2022

[18]Pathogenicity Analysis of Weaned Piglets Challenged With Novel Emerging Senecavirus A in Fujian, China. Cun Liu,Yanhan Liu,Xiubo Li,Lin Liang,Shangjin Cui. 2021

[19]IFIT3 mediated the type I interferon antiviral response by targeting Senecavirus A entry, assembly and release pathways. Fan Xu,Wen Dang,Tao Li,Yannan Wang,Fan Yang,Haixue Zheng. 2022

[20]Modeling senecavirus a replication in immortalized porcine alveolar macrophages triggers a robust interferon-mediated immune response that conversely constrains viral replication. Wen Dang,Tao Li,Fan Xu,Yannan Wang,Fan Yang,Haixue Zheng. 2023

作者其他论文 更多>>