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Based on the TLR4/NLRP3 Pathway and Its Impact on the Formation of NETs to Explore the Mechanism of Ginsenoside Rg1 on Acute Gouty Arthritis

文献类型： 外文期刊

作者： Zhiman Li;Yang Yu;Qiang Sun;Zhilong Li;Xiaohui Huo;Jiyue Sha;Di Qu;Yinshi Sun

作者机构：

关键词： acute gouty arthritis;ginsenoside Rg1;inflammatory response;neutrophil extracellular traps

期刊名称： International Journal of Molecular Sciences

ISSN： 1661-6596

年卷期： 2025 年 26 卷 9 期

页码：

收录情况： SCIE（2025版）

摘要： This study investigated whether ginsenoside Rg1 (G-Rg1) alleviated acute gouty arthritis (AGA) in rats by modulating the TLR4/NLRP3 pathway and neutrophil extracellular trap (NET) formation. Rats were orally administered G-Rg1 or colchicine (Col) for 7 days, and monosodium urate (MSU) was injected into the ankle joints on day 5 to induce AGA. Joint swelling, histopathology (HE staining), and serum markers (MPO, NE, MPO-DNA, IL-6, IL-1β; ELISA) were assessed at the baseline and 6–36 h post-modeling. Western blot and immunofluorescence analyzed the NET-related and TLR4/NLRP3 pathway proteins in synovial tissue. G-Rg1 significantly reduced ankle swelling and synovial inflammation compared with the AGA group, lowered the serum IL-6, IL-1β, MPO, NE, and MPO-DNA levels, and suppressed NET-associated protein expression. Mechanistically, G-Rg1 downregulated TLR4/NLRP3 pathway activation in synovial tissue. These findings suggest that G-Rg1 mitigates AGA by inhibiting TLR4/NLRP3 signaling, thereby reducing inflammatory cytokine release and NET formation.

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