数字农科院2.0

Transgenic tobacco plants expressing synthetic Cry1Ac and Cry1le genes are more toxic to cotton bollworm than those containing one gene

文献类型： 外文期刊

作者： Wang GuoYing;Lian Yun;Jia ZhiWei;Wang BaoMin;He KangLai;Song FuPing

作者机构：

关键词： insect resistance;transgenic crop

期刊名称： CHINESE SCIENCE BULLETIN

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年卷期：

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收录情况： SCI

摘要： Transgenic tobacco plants carrying Cry1Ac, Cry1le or both genes were obtained. In the leaves of transgenic plants carrying both genes, the contents of Cry1Ac and Cry1le proteins were 0.173% and 0.131% of the total proteins, respectively. Cry1Ac protein content was 0.182% and Cry1le protein content was 0.124% of the total proteins in the leaves of transgenic plants containing only one Bt gene. Fresh leaves of transgenic tobacco and wild-type plants were used for the insect bioassay against wild-type and Cry1Ac-resistant cotton bollworm (Helicoverpa armigera). The bioassay results showed that transgenic plants carrying both genes were significantly more toxic to wild-type and Cry1Ac-resistant cotton bollworm than those carrying Cry1Ac or Cry1le alone. This study indicates that the higher toxicity of transgenic tobacco plants carrying both genes is caused by the cooperative function of both Bt proteins, thus providing a potential way to delay the development of insect resistance to transgenic crops.

分类号： N1

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