数字农科院2.0

Removal of phenolic acid autotoxic substances from continuous cropping of capsicum: Biochar-immobilized laccase degradation performance and mechanism

文献类型： 外文期刊

作者： 张薛妍;;周吉恩;;吕诗羽;;原田;;杨璞;;姚彦坡;;梁军锋;;罗艳丽;;高同国;;王风

关键词： laccase; biochar; immobilization; phthalic acid; autotoxic substance; degradation

期刊名称： Advanced Composites and Hybrid Materials

ISSN： 2522-0128

年卷期： 2025 年

页码：

收录情况： SCIE（2025版） ; ; EI（2025版）

摘要： The accumulation of phenolic acid autotoxins is a key factor contributing to the challenges of continuous chili cropping, but efficient and stable remediation methods are currently lacking. This study innovatively employs K₂CO₃ alkali etching to prepare engineered biochar as a laccase carrier, achieving high-efficiency immobilization via glutaraldehyde cross-linking (maximum loading capacity: 177.3 U/g). Compared to free laccase, the immobilized system exhibits significant advantages: broader pH and temperature adaptability, enhanced long-term storage stability, and improved reusability. Experimental results demonstrate that 2 U/mL of immobilized laccase can completely degrade 20 mg/L phthalic acid within 10 hours and is effective against multiple chili autotoxic phenolic acids (ferulic acid, cinnamic acid, coumaric acid, and phydroxybenzoic acid). After phthalic acid degradation, the solution no longer inhibits chili seed germination, and soil experiments confirm a degradation rate of 51.07% after 11 days. Mechanistic studies reveal that the degradation process relies on synergistic radical and non radical pathways, with electron transfer playing a dominant role. LC-MS analysis confirms the transformation of phenolic acids into low-toxicity small molecules. This study is the first to propose a biochar-immobilized laccase synergistic degradation strategy, which offers high efficiency, environmental safety, sustainability, and broad applicability compared to existing methods.

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