数字农科院2.0

Development of a triplex TaqMan probe-based real-time PCR assay for simultaneous detection of swine influenza virus, porcine reproductive and respiratory syndrome virus, and porcine circovirus type 2

文献类型： 外文期刊

作者： Meng Jie Wang;Yu Pan;Wengjie Ma;Xingrong Wang;Changyou Xia;Yue Wang

作者机构：

关键词： Detection;PCV2;PRRSV;Real-time PCR;SIV

期刊名称： BMC Veterinary Research

ISSN： 1746-6148

年卷期： 2025 年 21 卷 1 期

页码：

收录情况： SCIE（2025版）

摘要： Background: Porcine respiratory disease complex poses a substantial economic challenge for the swine farms due to its prevalence in pig farming environments. The disease is primarily caused by viral pathogens such as swine influenza virus (SIV), porcine reproductive and respiratory syndrome virus (PRRSV), and porcine circovirus type 2 (PCV2). Given the high incidence and morbidity associated with these viruses, effective control strategies rely on rapid and accurate diagnosis. Results: To this end, we developed and validated a triplex TaqMan probe-based real-time PCR assay for the simultaneous detection and differentiation of SIV, PRRSV, and PCV2. This assay demonstrated high specificity, with no observed cross-reactivity between the target viruses. The assay’s sensitivity was determined to be 100 copies/µL for SIV and 10 copies/µL for PRRSV and PCV2. Comparison with a national standard detection method using 110 clinical samples revealed 100% agreement, confirming the reliability of the newly developed assay for clinical diagnostic applications. Conclusions: Given the zoonotic potential of SIV, this assay not only provides a valuable diagnostic tool for veterinary medicine but also contributes to enhanced public health surveillance efforts.

分类号：

  • 相关文献

[1]检测PCV2、PPV、PRV、PRRSV的多重PCR诊断方法的建立及初步应用. 岳丰雄,崔尚金,戚亭,周盛华,冉多良,张超范. 2008

[2]PCV2、PRV和PRRSV多重PCR方法的建立及其应用. 耿庆华,彭永刚,张波,裴程程. 2014

[3]葡萄卷叶相关病毒13在我国葡萄上的首次报道. 范旭东,张尊平,任芳,胡国君,董雅凤. 2019

[4]Development of TaqMan real-time PCR assay for detection and quantitation of reticuloendotheliosis virus. Li, Kai,Gao, Honglei,Gao, Li,Qi, Xiaole,Qin, Liting,Gao, Yulong,Xu, Yanwei,Wang, Xiaomei. 2012

[5]Highly Sensitive CRISPR/Cas12a-Based Fluorescence Detection of Porcine Reproductive and Respiratory Syndrome Virus. Siyuan Liu,Dagang Tao,Yuying Liao,Yalan Yang,Shouzhang Sun,Yulan Zhao,Peng Yang,Yijie Tang,Bin Chen,Yonggang Liu,Shengsong Xie,Zhonglin Tang. 2021

[6]Highly Sensitive CRISPR/Cas12a-Based Fluorescence Detection of Porcine Reproductive and Respiratory Syndrome Virus. Siyuan Liu,Dagang Tao,Yuying Liao,Yalan Yang,Shouzhang Sun,Yulan Zhao,Peng Yang,Yijie Tang,Bin Chen,Yonggang Liu,Shengsong Xie,Zhonglin Tang. 2021

[7]猪流感病毒M基因实时荧光定量PCR诊断方法的建立. 张春明,乔传玲,陈艳,杨焕良,辛晓光,陈化兰,冉多良. 2008

[8]猪流感病毒核蛋白基因的原核表达及其在诊断中的初步应用. 倪建强,张春玲,李海燕,崔尚金,仇华吉,王云峰,田志军,童光志. 2004

[9]PCV2感染SPF昆明小鼠动物模型的建立. 苗岚飞,崔尚金,张超范,胡守萍,陈长木,周盛华,岳丰雄,戚亭,谢红玲,张垚. 2008

[10]表达V5表位标签重组PCV2的构建及其生物学特性. 黄立平,陆月华,危艳武,郭龙军,吴洪丽,张飞雁,付玉洁,刘长明. 2013

[11]猪圆环病毒2型不同基因型毒株感染性克隆的构建及拯救病毒的体外生物学特性研究. 郭龙军,陆月华,危艳武,黄立平,刘长明. 2010

[12]猪圆环病毒Cap蛋白羧基端特异性单克隆抗体的制备. 马贯中,韩凌霞,朴范泽,刘怀然,司昌德,姜骞,曲连东. 2006

[13]猪圆环病毒2型不同基因型毒株感染性克隆的构建及拯救毒株的体外生物学特性. 郭龙军,陆月华,黄立平,危艳武,刘长明. 2011

[14]PCV2构象中和表位关键氨基酸的鉴定. 黄立平,陆月华,危艳武,郭龙军,吴洪丽,刘长明. 2013

[15]猪圆环病毒病基因工程疫苗研究进展. 高艳飞,王尚辉,王海伟,孙明霞,潘力,肖培宇,蔡雪辉,涂亚斌. 2022

[16]PCV2-HPS二联亚单位疫苗对小鼠免疫保护效果的评价. 栾慧,龚俊,赵馨雨,危艳武,李刚,刘长明,刘思国,张万江,王春来. 2023

[17]Co-expression of Ubiquitin gene and capsid protein gene enhances the potency of DNA immunization of PCV2 in mice. Fu, Fang,Li, Xuesong,Lang, Yuekun,Li, Xi,Li, Xuesong,Yang, Yuju,Tong, Guangzhi,Li, Guoxin,Zhou, Yanjun,Li, Xi. 2011

[18]Evaluation of the enhancing ability of three adjuvants for DNA vaccination using the porcine circovirus type 2 ORF2 (capsid) gene in mice. Fu, Fang,Lang, Yuekun,Li, Xuesong,Chai, Zheng,Liu, Siguo,Tian, Huabin,Li, Haizhong,Wang, Wei,Chen, Xin,Li, Xi,Li, Xuesong,Tong, Guangzhi.

[19]Virome of wild rats (Rattus norvegicus) captured far from pig farms in Jiangsu province of China reveals novel porcine circovirus type 2d (PCV2d) sequences. Min Zhao,Siwen Bao,Diandian Xu,Jingxian He,Han Zhang,Likai Ji,Shixing Yang,Xiaochun Wang,Quan Shen,Jia Liu,Qing Zhang,Xiao Ma,Wen Zhang,Tongling Shan. 2023

[20]A novel strategy for an anti-idiotype vaccine: nanobody mimicking neutralization epitope of porcine circovirus type 2. Deng, Yingying,Sheng, Yamin,Zhang, Guixi,Sun, Yani,Wang, Lei,Ji, Pinpin,Zhu, Jiahong,Wang, Gang,Liu, Baoyuan,Zhou, En-Min,Cai, Xuehui,Tu, Yabin,Hiscox, Julian A.,Stewart, James P.,Mu, Yang,Zhao, Qin. 2024

作者其他论文 更多>>