数字农科院2.0

Transcriptome Analysis of Early Lateral Root Formation in Tomato

文献类型： 外文期刊

作者： Zhang, Aiai;Shang, Qingmao

作者机构：

关键词： tomato;lateral root;auxin;transcriptome

期刊名称： PLANTS-BASEL

ISSN： 2223-7747

年卷期： 2024 年 13 卷 12 期

页码：

收录情况： SCIE（2024版）

摘要： Lateral roots (LRs) receive signals from the inter-root environment and absorb water and nutrients from the soil. Auxin regulates LR formation, but the mechanism in tomato remains largely unknown. In this study, 'Ailsa Craig' tomato LRs appeared on the third day and were unevenly distributed in primary roots. According to the location of LR occurrence, roots were divided into three equal parts: the shootward part of the root (RB), the middle part of the root (RM), and the tip part of the root (RT). Transverse sections of roots from days 1 to 6 revealed that the number of RB cells and the root diameter were significantly increased compared with RM and RT. Using roots from days 1 to 3, we carried out transcriptome sequencing analysis. Identified genes were classified into 16 co-expression clusters based on K-means, and genes in four associated clusters were highly expressed in RB. These four clusters (3, 5, 8, and 16) were enriched in cellulose metabolism, microtubule, and peptide metabolism pathways, all closely related to LR development. The four clusters contain numerous transcription factors linked to LR development including transcription factors of LATERAL ORGAN BOUNDRIES (LOB) and MADS-box families. Additionally, auxin-related genes GATA23, ARF7, LBD16, EXP, IAA4, IAA7, PIN1, PIN2, YUC3, and YUC4 were highly expressed in RB tissue. Free IAA content in 3 d RB was notably higher, reaching 3.3-5.5 ng/g, relative to RB in 1 d and 2 d. The LR number was promoted by 0.1 mu M of exogenous IAA and inhibited by exogenous NPA. We analyzed the root cell state and auxin signaling module during LR formation. At a certain stage of pericycle cell development, LR initiation is regulated by auxin signaling modules IAA14-ARF7/ARF19-LBD16-CDKA1 and IAA14-ARF7/ARF19-MUS/MUL-XTR6/EXP. Furthermore, as a key regulatory factor, auxin regulates the process of LR initiation and LR primordia (LRP) through different auxin signaling pathway modules.

分类号：

  • 相关文献

[1]Light Quality Regulates Lateral Root Development in Tobacco Seedlings by Shifting Auxin Distributions. Meng, Lin,Song, Wenjing,Dong, Jianxin,Wang, Chengdong,Xu, Yimin,Wang, Shusheng,Meng, Lin,Liu, Shangjun,Zhang, Yali.

[2]Potassium deficiency inhibits lateral root development in tobacco seedlings by changing auxin distribution. Song, Wenjing,Meng, Lin,Wang, Chengdong,Liu, Guangliang,Wang, Shusheng,Dong, Jianxin,Liu, Shangjun,Xue, Ren,Dong, Caixia,Zhang, Yali.

[3]Auxin and target of rapamycin spatiotemporally regulate root organogenesis. Xiulan Xie,Ying Wang,Raju Datla,Maozhi Ren. 2021

[4]The volatile cedrene from Trichoderma guizhouense modulates Arabidopsis root development through auxin transport and signalling. Li, Yucong,Shao, Jiahui,Fu, Yansong,Chen, Yu,Wang, Hongzhe,Xu, Zhihui,Feng, Haichao,Xun, Weibing,Liu, Yunpeng,Zhang, Nan,Shen, Qirong,Xuan, Wei,Zhang, Ruifu. 2021

[5]A PHYB-PIF4-auxin module promotes tomato graft formation in response to elevated ambient temperature. Meng, Xianmin,Zhang, Feng,Yang, Wencai,Shang, Qingmao. 2025

[6]Analysis of transcriptome in hickory (Carya cathayensis), and uncover the dynamics in the hormonal signaling pathway during graft process. Qiu, Lingling,Jiang, Bo,Fang, Jia,Shen, Yike,Fang, Zhongxiang,Kumar, Saravana R. M.,Yan, Daoliang,Zheng, Bingsong,Fang, Jia,Shen, Yike,Fang, Zhongxiang,Kumar, Saravana R. M.,Yan, Daoliang,Zheng, Bingsong,Yi, Keke,Shen, Chenjia. 2016

[7]Genetic dissection of the soybean dwarf mutant dm with integrated genomic, transcriptomic and methylomic analyses. Jian Song,Xuewen Wang,Lan Huang,Zhongfeng Li,Honglei Ren,Jun Wang. 2022

[8]Transcriptomic and metabolic changes during tassel branching development in maize. Yuxin Tai,Xiangling Lyu,Feng Pan,Lingzhi Meng,Zixiang Cheng,Zhennan Xu,Mingshun Li,Zhuanfang Hao,Degui Zhang,Hongjun Yong,Zhiqiang Zhou,Jienan Han,Xinhai Li,Jianfeng Weng. 2025

[9]Cr3a, a candidate gene conferring fruit cracking resistance, was fine-mapped in an introgression line of Solanum lycopersicum L.. Yifan Chen,Wenzheng Gao,Yu Zhu,Shuliang Qiu,Zhuoyao Qiu,Chenchen Dong,Ziteng Liu,Yongchen Du,Junming Li,Zejun Huang,Xin Li,Lei Liu,Liwang Liu,Xiaoxuan Wang. 2025

[10]Integration of transcriptome and metabolome reveals regulatory mechanisms of volatile flavor formation during tomato fruit ripening. Liu, Zhiqiang,Wu, Jianxin,Wang, Licai,Lu, Xiaonan,Ahammed, Golam Jalal,Zhang, Xiaolan,Cui, Xia,Wang, Haijing. 2025

[11]番茄基因组中抗病基因同源序列分离. 时涛,孙福在,回文广,李国庆. 2002

[12]蔬菜育种的基因组学. 黄三文. 2012

[13]感细菌性斑点病番茄品种上存有抗病基因pto序列. 时涛,李国庆,孙福在. 2004

[14]李宝聚博士诊病手记(八十八)番茄细菌性斑点病症状多样性与综合防治. 王莹莹,柴阿丽,李宝聚. 2015

[15]Identification and Characterization of the Growth-Regulating Factors-Interacting Factors in Cotton. Daowu Hu,Yuting Ge,Yinhua Jia,Shoupu He,Xiaoli Geng,Liru Wang,Zhaoe Pan,Zubair Iqbal,Tahir Mahmood,Hongge Li,Baojun Chen,Xiaoyang Wang,Baoyin Pang,Xiongming Du. 2022

[16]A genome-wide association study of lateral root number for Asian cotton (Gossypium arboreum L.). Hu Daowu,He Shoupu,Sun Gaofei,Jia Yinhua,Geng Xiaoli,Wang Liru,Pan Zhaoe,Chen Baojun,Li Hongge,Zubair, Iqbal,Wang Xiaoyang,Zhao Zibo,Ge Yuting,Pang Baoyin,Du Xiongming. 2022

[17]Overexpression of AcEXPA23 Promotes Lateral Root Development in Kiwifruit. Zhiyong Wu,Ming Li,Yunpeng Zhong,Lan Li,Dawei Cheng,Hong Gu,Xizhi Guo,Xiujuan Qi,Jinyong Chen. 2022

[18]Auxin Controlled By Ethylene Steers R.oot Development. Qin, Hua,Qin, Hua,Huang, Rongfeng,Huang, Rongfeng. 2018

[19]The Roles Of Auxin Biosynthesis Yucca Gene Family In Plants. Cheng, Jialing,Yang, Honglei,Liu, Li,Ma, Sang,Shang, Chunqiong,Ma, Sang,Cheng, Jialing,Liu, Li,Cao, Xu,Cao, Xu,Shang, Chunqiong,Yang, Honglei. 2019

[20]Genome-Wide Identification Of The Gharf G.ene Family Reveals That G harf2 And Gharf18 Are Involved In Cotton Fibre Cell Initiation. He, Peng,Liu, Hao,Xiao, Guanghui,Pang, Chaoyou,Xiao, Guanghui,Xiao, Guanghui,Xiao, Guanghui,Yu, Jianing,Zhang, Li,Zhao, Peng. 2018

作者其他论文 更多>>