数字农科院2.0

Comparative Transcriptome Analysis Reveals Differential Gene Expression in Resistant and Susceptible Watermelon Varieties in Response to Meloidogyne incognita

文献类型： 外文期刊

作者： Yingchun Zhu;Gaopeng Yuan;Renzong Zhao;Guolin An;Weihua Li;Wenjing Si;Junpu Liu;Dexi Sun

作者机构：

关键词： comparative transcriptome;Meloidogyne incognita;molecular mechanism;resistant;susceptible;watermelon

期刊名称： Life

ISSN： 2075-1729

年卷期： 2022 年 12.0 卷 7 期

页码：

摘要： M. incognita is a major parasitic plant disease in watermelon production, causing serious economic losses. Although there are many studies on root-knot nematode, the resistance mechanism is still unclear. In this study, in order to fully understand the mechanism of watermelon resistance to root-knot nematode, the relatively strongly resistant ‘Hongzi watermelon’ variety and the susceptible ‘M16’ watermelon variety were used as materials, combined with RNA sequencing (RNA-seq), to analyze the expression abundance of resistant and susceptible varieties at 0, 2, 8 and 15 days post-infection (DPI) by M. incognita. The number of differentially expressed genes (DEGs) in the four comparison groups (A0_B0, A1_B1, A2_B2 and A3_B3) was 3645, 2306, 4449 and 2362, respectively, and there were 835 shared DEGs among them. GO annotation and KEGG pathway enrichment analysis showed that 835 DEGs were mainly involved in phenylpropane biosynthesis and carbon metabolism. Furthermore, lignin-biosynthesis-related genes (4CL (4-coumaric acid-CoA ligase), C3H (coumaric acid 3-hydroxylase), CSE (caffeoyl shikimate esterase), COMT (caffeic acid-O-methyltransferase), CCR (cinnamyl CoA reductase) and PRX (peroxidase)), defense-related proteins (UDP-glucoronosyl/UDP-glucosyl transferase, UGT84A13; salicylic acid binding protein, SABP2) and some transcription factors (TFs) were highlighted, which may be potential candidate genes for further analysis in the infection process of M. incognita. These results suggest that watermelon can achieve resistance to M. incognita by increasing the content of lignin and phenols in root or improving ROS level. These RNA-seq data provide new knowledge for future functional studies and will be helpful to further elucidate the molecular mechanism of resistance to M. incognita in watermelon.

分类号：

  • 相关文献

[1]Comparative Transcriptome Profiling Provides Insights into Plant Salt Tolerance in Watermelon (Citrullus lanatus). Zhu, Yingchun,Yuan, Gaopeng,Gao, Bowen,An, Guolin,Li, Weihua,Si, Wenjing,Sun, Dexi,Liu, Junpu. 2022

[2]Comparative Analysts of Flower Proteomes of Two Apple Genotypes Selected by their Different Resistance to Alternaria alternate. Zhang, Cai-xia,Zhang, Li-yi,Tian, Yi,Cong, Pei-hua,Zhang, Cai-xia,Zhang, Li-yi,Tian, Yi,Cong, Pei-hua.

[3]iTRAQ-based proteomics of sunflower cultivars differing in resistance to parasitic weed Orobanche cumana. Yang, Chong,Zhang, Na,Islam, Faisal,Song, Wenjian,Hu, Luyang,Zhou, Weijun,Xu, Ling,Liu, Dan,Xie, Xiaonan.

[4]Transcriptional Analysis on Resistant and Susceptible Kiwifruit Genotypes Activating Different Plant-Immunity Processes against Pseudomonas syringae pv. actinidiae. Xiaobo Qin,Min Zhang,Qiaohong Li,Dalei Chen,Leiming Sun,Xiujuan Qi,Ke Cao,Jinbao Fang. 2022

[5]西瓜EST资源的SSR信息分析及标记开发. 赵胜杰,刘文革,阎志红,何楠,包文风. 2009

[6]施用钾肥对西瓜番茄红素含量的影响. 何楠,赵胜杰,刘文革,阎志红,万学闪. 2009

[7]NaCl胁迫对不同西瓜种质发芽的影响. 阎志红,刘文革,赵胜杰,何楠,王俊良. 2009

[8]不同倍性西瓜果实Vc含量比较研究. 程志强,刘文革,刘志敏,阎志红,赵胜杰,何楠,张俊杰. 2009

[9]不同染色体倍性西瓜花粉形态观察比较. 刘文革,王鸣,阎志红. 2009

[10]Characterization of glyphosate-resistant goosegrass (Eleusine indica) populations in China. Huang Hong-juan,Wei Shou-hui,Zhang Chao-xian,Huang Zhao-feng. 2015

[11]ELEVATED ENDOGENOUS ISOPENTENYL ADENINE CONTENT IS CORRELATED WITH AN EXTREMELY SHOOTY RICE PHENOTYPE. ZHU, YX,QIN, RZ,SHAN, XY,CHEN, ZL.

[12]An ethanol-tolerant recombinant Escherichia coli expressing Zymomonas mobilis pdc and adhB genes for enhanced ethanol production from xylose. Chen, Ming,Xu, Yuquan,Li, Shuying,Lu, Wei,Ping, Shuzhen,Zhang, Wei,Lin, Min,Wang, Zhanchao.

[13]Altered mating behaviour in a Cry1Ac-resistant strain of Helicoverpa armigera (Lepidoptera : Noctuidae). Zhao, X. C.,Wu, K. M.,Liang, G. M.,Guo, Y. Y.. 2008

[14]Knockout of ABC Transporter ABCG4 Gene Confers Resistance to Cry1 Proteins in Ostrinia furnacalis. Qing Gao,Yaling Lin,Xiuping Wang,Dapeng Jing,Zhenying Wang,Kanglai He,Shuxiong Bai,Yongjun Zhang,Tiantao Zhang. 2022

[15]Development of bottle gourd lines resistant to zucchini yellow mosaic virus using ethyl methanesulfonate mutagenesis. Asma Mohammed Saeed Al-Kubati,Baoshan Kang,Liming Liu,Aqleem Abbas,Qinsheng Gu. 2021

[16]Establishment and application of TaqMan real-time PCR method for detection of Theileria annulata resistant to buparvaquone. Su S.,Zhao S.,Liu J.,Zhang C.,Zhu H.,Guan G.,Yin H.,Luo J.. 2024

[17]Stress-induced higher vein density in the C-3-C-4 intermediate Moricandia suffruticosa under drought and heat stress. Zhu, Xiaoyi,Liu, Jun,Sun, Xingchao,Kuang, Chen,Liu, Hongfang,Zhang, Liang,Zheng, Qiwei,Liu, Jing,Li, Jun,Wang, Hanzhong,Hua, Wei. 2022

[18]Comparative Transcriptome Analysis Reveals the Mechanisms Underlying Differences in Salt Tolerance Between indica and japonica Rice at Seedling Stage. Weilong Kong,Tong Sun,Chenhao Zhang,Xiaoxiao Deng,Yangsheng Li. 2021

[19]Comparative Transcriptome Analyses of the Developmental Stages of Taenia multiceps. Wen Hui Li,Yang Yang,Nian Zhang Zhang,Jian Kui Wang,Yin Ju Liu,Li Li,Hong Bin Yan,Wan Zhong Jia,Baoquan Fu. 2021

[20]Comparative Transcriptome Analyses of the Developmental Stages of Taenia multiceps. Wen Hui Li,Yang Yang,Nian Zhang Zhang,Jian Kui Wang,Yin Ju Liu,Li Li,Hong Bin Yan,Wan Zhong Jia,Baoquan Fu. 2021

作者其他论文 更多>>