数字农科院2.0

Sweet cherry AP2/ERF transcription factor, PavRAV2, negatively modulates fruit size by directly repressing PavKLUH expression

文献类型： 外文期刊

作者： Xiliang Qi;Lifeng Liu;Congli Liu;Lulu Song;Yuanxin Dong;Lei Chen;Ming Li

作者机构：

关键词： fruit size;gene silencing;PavKLUH;PavRAV2;promoter;sweet cherry

期刊名称： Physiologia Plantarum

ISSN： 0031-9317

年卷期： 2023 年 175 卷 6 期

页码：

收录情况： SCIE（2023版）

摘要： For sweet cherry, fruit size is one of the main targets in breeding programs owing to the high market value of larger fruits. KLUH/CYP78A5 is an important regulator of seed/fruit size in several plant species, but its molecular mechanism is largely unknown. In this study, we characterized the function of PavKLUH in the regulation of sweet cherry fruit size. The ectopic overexpression of PavKLUH in Arabidopsis increased the size of its siliques and seeds, whereas virus-induced gene silencing of PavKLUH in sweet cherry significantly decreased fruit size by restricting mesocarp cell expansion. We screened out an AP2/ERF transcription factor containing a B3-like domain, designated as PavRAV2, which was able to physically interact with PavKLUH promoter in a yeast one-hybrid (Y1H) system. In Y1H assays, electrophoretic mobility shift assays, and dual-luciferase reporter analyses, PavRAV2 directly bound to the promoter of PavKLUH in vitro and in vivo, and suppressed PavKLUH expression. Silencing of PavRAV2 resulted in enlarged fruit as a result of enhanced mesocarp cell expansion. Together, our results provide new insights into signaling pathways related to fruit size, and outline a possible mechanism for how the RAV transcription factor directly regulates CYP78A family members to influence fruit size and development.

分类号：

  • 相关文献

[1]A sweet cherry AGAMOUS-LIKE transcription factor PavAGL15 affects fruit size by directly repressing the PavCYP78A9 expression. Yuanxin Dong,Xiliang Qi,Congli Liu,Lulu Song,Li Ming. 2022

[2]A Sweet Cherry Glutathione S-Transferase Gene, PavGST1, Plays a Central Role in Fruit Skin Coloration. Xiliang Qi,Congli Liu,Lulu Song,Yuanxin Dong,Lei Chen,Ming Li. 2022

[3]EIAV基因转移载体启动子的比较. 韩凌霞,李亚明,曲连东,司昌德,姜骞,刘家森. 2006

[4]PacMYBA, a sweet cherry R2R3-MYB transcription factor, is a positive regulator of salt stress tolerance and pathogen resistance. Shen, Xinjie,Guo, Xinwei,Guo, Xiao,Zhao, Di,Li, Tianhong,Shen, Xinjie,Zhao, Wei,Chen, Jingsheng,Li, Tianhong.

[5]Outdoor color rating of sweet cherries using computer vision. Wang, Qi,Wang, Hui,Xie, Lijuan,Zhang, Qin,Wang, Hui,Xie, Lijuan.

[6]Role of abscisic acid and ethylene in sweet cherry fruit maturation: molecular aspects. Ren, J.,Chen, P.,Dai, S. J.,Li, P.,Li, Q.,Ji, K.,Wang, Y. P.,Leng, P.,Ren, J.. 2011

[7]Exogenous Melatonin Delays Postharvest Fruit S.enescence And Maintains The Q uality Of Sweet Cherries. Wang, Feng,Wang, Feng,Wang, Feng,Zhao, Qifeng,Zhang, Xiaoping,Yang, Qingzhen. 2019

[8]Pacmyba, A Sweet Cherry R2R3-Myb T.ranscription Factor, Is A P ositive Regulator Of Salt Stress Tolerance And Pathogen Resistance. Shen, XJ, Guo, XW, Guo, X, Zhao, D, Zhao, W, Chen, JS, Li, TH. 2017

[9]A 5.2-kb insertion in the coding sequence of PavSCPL, a serine carboxypeptidase-like enhances fruit firmness in Prunus avium. Qi, Xiliang,Dong, Yuanxin,Liu, Congli,Song, Lulu,Chen, Lei,Li, Ming. 2024

[10]Physicochemical Properties of Geographical Indication (GI) Sweet Cherries in China and Their Influencing Factors of Cultivar, Climate Type, and Soil Condition. Ying Nie,Jiazhang Huang,Rui Liu,Pei Wang,Peng Liu,Man Lu,Junmao Sun. 2023

[11]Malate accumulation and transcriptome patterns during fruit development in sweet Cherry (Prunus avium L.). Congli Liu,Lei Chen,Xiliang Qi,Lulu Song,Manqing Wang,Shu Han,Ming Li. 2025

[12]Effected Cultural Practices on Fruit Quality and Polyphenols Content. Khanizadeh, S.,Charles, M. T.,Fan, L.,Yu, C.,Tao, S.,Fan, L.. 2011

[13]Cloning, localization and expression analysis of two fw2.2-like genes in small- and large-fruited pear species. Tian Jia,Zeng Bin,Li Jiang,Luo Shu-ping,Li Xiu-gen,Wu Bin,Wu Bin.

[14]QTLs and candidate genes analyses for fruit size under domestication and differentiation in melon (Cucumis melo L.) based on high resolution maps. Lian Qun,Fu Qiushi,Xu Yongyang,Hu Zhicheng,Zheng Jing,Zhang Aiai,He Yuhua,Wang Changsheng,Xu Chuanqiang,Chen Benxue,Garcia-Mas Jordi,Zhao Guangwei,Wang Huaisong. 2021

[15]QTLs and candidate genes analyses for fruit size under domestication and differentiation in melon (Cucumis melo L.) based on high resolution maps. Lian Qun,Fu Qiushi,Xu Yongyang,Hu Zhicheng,Zheng Jing,Zhang Aiai,He Yuhua,Wang Changsheng,Xu Chuanqiang,Chen Benxue,Garcia-Mas Jordi,Zhao Guangwei,Wang Huaisong. 2021

[16]Transcriptome analysis of transgenic apple fruit overexpressing microRNA172 reveals candidate transcription factors regulating apple fruit development at early stages. Zhe Zhou,Yanmin Zhu,Hengtao Zhang,Ruiping Zhang,Qiming Gao,Tiyu Ding,Huan Wang,Zhenli Yan,Jia Long Yao. 2021

[17]QTLs and candidate genes analyses for fruit size under domestication and differentiation in melon (Cucumis melo L.) based on high resolution maps. Lian Qun,Fu Qiushi,Xu Yongyang,Hu Zhicheng,Zheng Jing,Zhang Aiai,He Yuhua,Wang Changsheng,Xu Chuanqiang,Chen Benxue,Garcia-Mas Jordi,Zhao Guangwei,Wang Huaisong. 2021

[18]Mapping and validation of a new quantitative trait locus (QTL) for fruit size in watermelon (Citrullus lanatus). Na Li,Shengnan Kong,Dan Zhou,Nannan Li,Jianli Shang,Jiming Wang,Shuangwu Ma. 2023

[19]ASYMMETRIC LEAVES 2 and ASYMMETRIC LEAVES 2-LIKE are partially redundant genes and essential for fruit development in tomato. Dong, Rongrong,Yuan, Yaqin,Liu, Zhiqiang,Sun, Shuai,Wang, Haijing,Ren, Huazhong,Cui, Xia,Li, Ren. 2023

[20]Vascular network in the pericarp of tomato fruit and implications for fruit size, quality, and drought response. Hou, Xuemin,Li, Hao,Bertin, Nadia,Brodribb, Tim J.,Du, Taisheng. 2025

作者其他论文 更多>>