数字农科院2.0

Functional Analysis of the Pathogenesis-Related Protein 1 (CaPR1) Gene in the Pepper Response to Chilli veinal mottle virus (ChiVMV) Infection

文献类型： 外文期刊

作者： Huang, Chunzi;Zhao, Zengjing;Wu, Xing;Zhao, Hu;Wang, Meng;He, Zhi;Li, Zongjun;Wang, Lihao;Tang, Yafei;Wang, Risheng;He, Longfei;Gong, Mingxia

作者机构：

关键词： pepper;PR1 gene;ChiVMV;functional analysis

期刊名称： VIRUSES-BASEL

ISSN：

年卷期： 2025 年 17 卷 11 期

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收录情况： SCIE（2025版）

摘要： Chilli veinal mottle virus (ChiVMV) causes severe yield losses in pepper across Asia. It is very urgent to study the host plant resistance to control this viral disease. As a type of defense response gene, pathogenesis-related protein 1 (PR1) is a well-established defense marker against fungal/bacterial pathogens, and its role in virus resistance remains unclear. Here, we cloned CaPR1 from the ChiVMV-highly resistant pepper variety 'Perennial'. The 477 bp ORF encodes a 17.65 kDa basic protein containing a conserved CAP-PR1 domain. The subcellular localization of CaPR1 revealed that it was located in the plasma membrane, endoplasmic reticulum (ER), and nucleus. RT-qPCR revealed leaf-predominant expression, with earlier and stronger induction in the highly resistant than the highly susceptible variety after ChiVMV inoculation (6.4-fold at 2 days post-inoculation). The overexpression of CaPR1 in tobacco significantly increased resistance, reducing disease index by 25% and viral coat protein accumulation. Our findings identified CaPR1 as a positive regulator of ChiVMV resistance, providing a molecular target for pepper breeding. In addition, exogenous SA treatment increased the resistance of the highly susceptible cultivar 'Guijiao 12' to ChiVMV, and 0.25 mM had a greater effect.

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