数字农科院2.0

The Deletion of the MGF360-10L/505-7R Genes of African Swine Fever Virus Results in High Attenuation but No Protection Against Homologous Challenge in Pigs

文献类型： 外文期刊

作者： Linlin Zheng;Zilong Yan;Xiaolan Qi;Jingjing Ren;Zhao Ma;Huanan Liu;Zhao Zhang;Dan Li;Jingjing Pei;Shuqi Xiao;Tao Feng;Xinglong Wang;Haixue Zheng

作者机构：

关键词： ASFV;attenuated strain;immunoprotection;MGF360-10L;MGF505-7R

期刊名称： Viruses

ISSN： 1999-4915

年卷期： 2025 年 17 卷 2 期

页码：

收录情况： SCIE（2025版）

摘要： African swine fever virus (ASFV) is the causative agent of African swine fever (ASF), a severe hemorrhagic disease with a mortality rate reaching 100%. Despite extensive research on ASFV mechanisms, no safe and effective vaccines or antiviral treatments have been developed. Live attenuated vaccines generated via gene deletion are considered to be highly promising. We developed a novel recombinant ASFV strain by deleting MGF360-10L and MGF505-7R, significantly reducing virulence in pigs. In the inoculation experiment, pigs were infected with 104 50% hemadsorption doses (HAD50) of the mutant strain. All the animals survived the observation period without showing ASF-related clinical signs. Importantly, no significant viral infections were detected in the cohabitating pigs. In the virus challenge experiment, all pigs succumbed after being challenged with the parent strain. RNA-seq analysis showed that the recombinant virus induced slightly higher expression of natural immune factors than the parent ASFV; however, this level was insufficient to provide immune protection. In conclusion, our study demonstrates that deleting MGF360-10L and MGF505-7R from ASFV CN/GS/2018 significantly reduces virulence but fails to provide protection against the parent strain.

分类号：

  • 相关文献

[1]Development of a new effective African swine fever virus vaccine candidate by deletion of the H240R and MGF505-7R genes results in protective immunity against the Eurasia strain. Li, Jiangnan,Song, Jie,Zhou, Shijun,Li, Shuai,Liu, Jia,Li, Tingting,Zhang, Zhaoxia,Zhang, Xianfeng,He, Xijun,Chen, Weiye,Zheng, Jun,Zhao, Dongming,Bu, Zhigao,Huang, Li,Weng, Changjiang. 2023

[2]Sequential Deletions of Interferon Inhibitors MGF110-9L and MGF505-7R Result in Sterile Immunity against the Eurasia Strain of Africa Swine Fever. Ding, Mingyang,Dang, Wen,Liu, Huanan,Zhang, Keshan,Xu, Fan,Tian, Hong,Huang, Huaguo,Shi, Zhengwang,Sunkang, Yongjie,Qin, Xiaodong,Zhang, Yong,Zheng, Haixue. 2022

[3]Combinational Deletions of MGF360-9L and MGF505-7R Attenuated Highly Virulent African Swine Fever Virus and Conferred Protection against Homologous Challenge. Ding, Mingyang,Dang, Wen,Liu, Huanan,Xu, Fan,Huang, Huaguo,Sunkang, Yongjie,Li, Tao,Pei, Jingjing,Liu, Xiangtao,Zhang, Yong,Zheng, Haixue. 2022

[4]ASFV解旋酶D1133L蛋白表达和抗体检测方法的建立及初步应用. 侯景,申超超,张大俊,杨博,史喜绢,张婷,杨泽晓,张克山,郑海学,李丹,党文,刘湘涛. 2021

[5]非洲猪瘟病毒多基因家族MGF360-13L基因功能的初步研究. 陈达年,马旭升,代军飞,汪洋,李茜,白衡,毛甜甜,刘永生,丁龙,陈昊翰,陈思言,饶宇飞,贾宁,张杰,郑海学,刘湘涛. 2022

[6]非洲猪瘟病毒E184L蛋白的原核表达及单克隆抗体的制备. 李雪雯,李婷婷,李长尧,焦爽,郑君,荣俊,翁长江. 2023

[7]Characterization of aspartyl aminopeptidase from Schistosoma japonicum. Zheng Shang,Qinghong Guo,Xue Zhou,Yongcheng Yue,Kerou Zhou,Liying Tang,Zhizhong Zhang,Zhiqiang Fu,Jinming Liu,Jiaojiao Lin,Bin Xu,Min Zhang,Yang Hong. 2022

[8]Evaluation of the immunoprotective effects of porcine deltacoronavirus subunit vaccines. Yu R.,Zhang L.,Zhou P.,Zhang Z.,Liu X.,Wang Y.,Guo H.,Pan L.,Liu X.. 2024

[9]Single self-cleaving mRNA vaccine expressing multiple viral structural proteins elicits robust immune responses and protects nursing piglets against PDCoV infection. Yu, Ruiming,Bai, Yingjie,Zhang, Liping,Zhou, Peng,Zhang, Zhongwang,Yang, Jun,Lu, Yanzhen,Wang, Dongsheng,Peng, Yousheng,Li, Dan,He, Jian,Wang, Yonglu,Zhang, Quanwei,Yuan, Ligang,Guo, Huichen,Pan, Li,Liu, Xinsheng. 2025

[10]Pathogenicity comparison of the SMPV-11 and attenuated mink enteritis virus F61 in mink. Yang Wang,Bo Hu,Rongguang Lu,Fanshu Ma,Shuang Lv,Hailing Zhang,Xue Bai,Lei Zhang,Ning Shi,Xintong Li,Sining Fan,Shizhen Lian,Xijun Yan,Yanzhu Zhu. 2021

[11]Pathogenicity comparison of the SMPV-11 and attenuated mink enteritis virus F61 in mink. Yang Wang,Bo Hu,Rongguang Lu,Fanshu Ma,Shuang Lv,Hailing Zhang,Xue Bai,Lei Zhang,Ning Shi,Xintong Li,Sining Fan,Shizhen Lian,Xijun Yan,Yanzhu Zhu. 2021

[12]Cryo-EM structures of infectious bursal disease viruses with different virulences provide insights into their assembly and invasion. Keyan Bao,Xiaole Qi,Yan Li,Minqing Gong,Xiaomei Wang,Ping Zhu. 2022

[13]Deletion of African Swine Fever Virus (ASFV) H240R Gene Attenuates the Virulence of ASFV by Enhancing NLRP3-Mediated Inflammatory Responses. Huang, Li,Liu, Hongyang,Ye, Guangqiang,Liu, Xiaohong,Chen, Weiye,Wang, Zilong,Zhao, Dongming,Zhang, Zhaoxia,Feng, Chunying,Hu, Liang,Yu, Huibin,Zhou, Shijun,Zhang, Xianfeng,He, Xijun,Zheng, Jun,Bu, Zhigao,Li, Jiangnan,Weng, Changjiang. 2023

[14]African Swine Fever Virus H240R Protein Inhibits the Production of Type I Interferon through Disrupting the Oligomerization of STING. Ye, Guangqiang,Liu, Hongyang,Liu, Xiaohong,Chen, Weiye,Li, Jiangnan,Zhao, Dongming,Wang, Gang,Feng, Chunying,Zhang, Zhaoxia,Zhou, Qiongqiong,Zheng, Jun,Bu, Zhigao,Weng, Changjiang,Huang, Li. 2023

[15]African swine fever virus E120R protein inhibits interferon beta production by interacting with IRF3 to block its activation. Huisheng Liu,Zixiang Zhu,Tao Feng,Zhao Ma,Qiao Xue,Panxue Wu,Pan Li,Shasha Li,Fan Yang,Weijun Cao,Zhaoning Xue,Hongjun Chen,Xiangtao Liu,Haixue Zheng. 2021

[16]Development and in vivo evaluation of MGF100-1R deletion mutant in an African swine fever virus Chinese strain. Yingnan Liu,Yao Li,Zhenhua Xie,Qingying Ao,Dongdong Di,Wanqi Yu,Lu Lv,Qiuping Zhong,Yingying Song,Xinxin Liao,Qingqing Song,Heng Wang,Hongjun Chen. 2021

[17]Identification of one novel epitope targeting p54 protein of African swine fever virus using monoclonal antibody and development of a capable ELISA. Yunlei Cao,Dongmei Han,Yujiao Zhang,Kuan Zhang,Nannan Du,Wu Tong,Guoxin Li,Hao Zheng,Changlong Liu,Fei Gao,Guangzhi Tong. 2021

[18]Multifunctional pMGF505-7R Is a Key Virulence-Related Factor of African Swine Fever Virus. Li Huang,Jiangnan Li,Jun Zheng,Dan Li,Changjiang Weng. 2022

[19]African swine fever virus f317l protein inhibits nf-k b activation to evade host immune response and promote viral replication. Jinping Yang,Shasha Li,Tao Feng,Xiangle Zhang,Fan Yang,Weijun Cao,Hongjun Chen,Huisheng Liu,Keshan Zhang,Zixiang Zhu,Haixue Zheng. 2021

[20]Genome-wide transcriptomic analysis of highly virulent African swine fever virus infection reveals complex and unique virus host interaction. Xiaohui Ju,Fang Li,Jingrui Li,Chunyan Wu,Guangtao Xiang,Xiaomin Zhao,Yuchen Nan,Dongming Zhao,Qiang Ding. 2021

作者其他论文 更多>>