数字农科院2.0

Potato expressing Cry1C and Cry2A confers resistance to Phthorimaea operculella (Lepidoptera: Gelechiidae)

文献类型： 外文期刊

作者： Junjie Yan; Zaihui Zhou; Angharad M. R. Gatehouse; Weihua Ma;; Yulin Gao

关键词： Bt protein; Cry1C; Cry2A; Phthorimaea operculella; potato; potato tuber moth

期刊名称： PEST MANAGEMENT SCIENCE

ISSN： 1526-498X

年卷期： 2025 年

页码：

收录情况： SCIE（2025版）

摘要： BACKGROUND As the third most important global food crop, potato plays a vital role in ensuring food security and poverty alleviation. However, its production is severely threatened by the potato tuber moth (Phthorimaea operculella; PTM), a destructive pest that damages foliage during growth and bores into tubers during storage, causing yield losses of 85–100% under severe infestations. Traditional reliance on chemical pesticides poses challenges such as environmental pollution, pesticide residues, and increased production costs, highlighting the need for sustainable alternatives. RESULT In this study, Cry1C and Cry2A genes were expressed in potato cultivar E3 via Agrobacterium-mediated transformation, generating single-copy transgenic lines with high gene expression. Bioassays showed that PTM larvae feeding on transgenic leaves exhibited significantly elevated mortality (>35.4%), with the Cry1C-2 line achieving 60.4% mortality within 24 h. Notably, the highest-expressing Cry1C and Cry2A transgenic lines caused 100% larval mortality within 4 and 7 days, respectively, demonstrating complete lethality against PTM. Histological analysis confirmed that Bt proteins induced midgut epithelial cell lysis and peritrophic membrane disruption, directly leading to insect death. CONCLUSION This study is the first to demonstrate that Cry2A expression in potato confers resistance to PTM. It provides novel genetic resources for insect-resistant potato breeding and proposes a gene pyramiding strategy to delay the onset of resistance evolution in pest populations. Future research should focus on evaluating field resistance durability and investigating potential synergistic effects between Cry1C/Cry2A and other Bt proteins to develop multi-target pest management systems. © 2025 Society of Chemical Industry.

分类号：

  • 相关文献

[1]Safety Assessment Of Bacillus Thuringiensis I.nsecticidal Proteins Cry1C And C ry2A With A Zebrafish Embryotoxicity Test. Chen, Xiu-Ping,Gao, Yan-Jie,Li, Yun-He,Peng, Yu-Fa,Chen, Yi,Zhu, Hao-Jun,Zhu, Hao-Jun,Chen, Yi. 2018

[2]Ingestion Of Bt Rice Pollen D.oes Notreduce The Survivalorhypopharyngeal G land Development Of Apis Mellifera Adults. Wang, YY, Dai, PL, Chen, XP, Romeis, J, Shi, JR, Peng, YF, Li, YH. 2017

[3]Potential Of A Strain Of B.eauveria Bassiana (Hypocreales: Cordycipitaceae) F or The Control Of The Potato Tuberworm, Phthorimaea Operculella (Zeller). Gao, Yulin,Lei, Zhongren,Yuan, Huiguo,Rondon, Silvia I.. 2017

[4]Potential Of Steinernema Carpocapsae (Weiser) As A Biological Control Agent Against Potato Tuber Moth, Phthorimaea Operculella (Zeller) (Lepidoptera: Gelechiidae). Reitz, Stuart,Yan Jun-jie,Gao Yu-lin,Sarkar, Shovon Chandra,Yan Jun-jie,Meng Rui-xia. 2020

[5]Identification and Functional Characterization of Two Putative Pheromone Receptors in the Potato Tuber Moth, Phthorimaea operculella. Xiaoli He,Yajie Cai,Jinglei Zhu,Mengdi Zhang,Yadong Zhang,Yang Ge,Zengrong Zhu,Wenwu Zhou,Guirong Wang,Yulin Gao. 2021

[6]Exploiting the ‘ecology of fear’: volatilemediated disruption of moth development and reproduction by a mite predator. Bing-Xia Feng,,,,Guang Zou,,,, Mao-Fa Yang,,,,Guy Smagghe,,,,Jian-Feng Liu,,,,Yu-Lin Gao. 2025

[7]Influence of transgenic rice expressing a fused Cry1Ab/1Ac protein on frogs in paddy fields. Chen, Xiu-Ping,Zhu, Hao-Jun,Peng, Yu-Fa,Wang, Jia-Mei,Zhu, Hao-Jun,Ding, Jia-Tong,Liang, Yu-Yong. 2014

[8]Dynamics of microbial population size in rhizosphere soil of Monsanto's Cry1Ac cotton. Zhang, Y. -J.,Xie, M.,Peng, D. -L.,Zhao, J. -J.,Zhang, Z. -R..

[9]A 90 Day Safety Assessment of Genetically Modified Rice Expressing Cry1Ab/1Ac Protein using an Aquatic Animal Model. Wang, Jia-Mei,Ding, Jia-Tong,Zhu, Hao-Jun,Chen, Yi,Li, Yun-He,Wang, Jia-Mei,Chen, Xiu-Ping,Peng, Yu-Fa.

[10]Resistance monitoring of Helicoverpa armigera (Lepidoptera : Noctuidae) to Bacillus thuringiensis insecticidal protein in China. Guo, YY,Lv, N,Greenplate, JT,Deaton, R. 2002

[11]Safety Of Bacillus Thuringiensis Cry1C P.rotein For Daphnia Magna B ased On Different Functional Traits. Romeis, Jorg,Peng, Yufa,Chen, Xiuping,Zhu, Haojun,Yang, Yan,Zhu, Haojun,Chen, Yi,Romeis, Jorg,Li, Yunhe. 2018

[12]Synergistic interaction of Cry1Ah and Vip3Aa19 proteins combination with midgut ATP-binding cassette subfamily C receptors of Conogethes punctiferalis (Guenée) (Lepidoptera: Crambidae). Su Mon Shwe,Sivaprasath Prabu,Dapeng Jing,Kanglai He,Zhenying Wang. 2022

[13]Effects Of Straw Leachates From C.ry1C-Expressing Transgenic Rice On T he Development And Reproduction Of Daphnia Magna. Chen, Yi,Chen, Xiuping,Peng, Yufa,Li, Yunhe,Romeis, Jorg,Zhu, Haojun,Gao, Yanjie,Chen, Yi,Zhu, Haojun,Romeis, Jorg. 2018

[14]Sensitivities of Fall Armyworm (Spodoptera frugiperda) Populations in Different Regions of China to Four Bt Proteins. Yanfang Zhou,Chunmeng Huang,Yi Chen,Lanzhi Han,Jiajian Xie,Xiuping Chen. 2023

[15]Response of the Propylea japonica Microbiota to Treatment with Cry1B Protein. Fengchao Diao,Yarong Li,Xueke Gao,Junyu Luo,Xiangzhen Zhu,Li Wang,Kaixin Zhang,Dongyang Li,Jichao Ji,Jinjie Cui. 2023

[16]Potato Farmers' Preference For Agricultural Insurance In China: An Investigation Using The Choice Experimental Method. Guo Yan-zhi,Alec Zuo,Huang Ze-ying,Sun Jun-mao. 2020

[17]Tracking Disease Resistance Deployment In P.otato Breeding By Enrichment S equencing. Armstrong, MR, Vossen, J, Lim, TY, Hutten, RCB, Xu, JF, Strachan, SM, Harrower, B, Champouret, N, Gilroy, EM, Hein, I. 2019

[18]The Estimation Of Crop Emergence I.n Potatoes By Uav R gb Imagery. Li, B, Xu, XM, Han, JW, Zhang, L, Bian, CS, Jin, LP, Liu, JG. 2019

[19]Reserving Winter Snow For The R.elief Of Spring Drought B y Film Mulching In Northeast China. Jia, HC, Zhang, Y, Tian, SY, Emon, RM, Yang, XY, Yan, HR, Wu, TT, Lu, WC, Siddique, KHM, Han, TF. 2017

[20]Effects Of (S)-Carvone And Gibberellin O.n Sugar Accumulation In P otatoes During Low Temperature Storage. Xie, YJ, Onik, JC, Hu, XJ, Duan, YQ, Lin, Q. 2018

作者其他论文 更多>>