Loop PDE of viral capsid protein is involved in immune escape of the emerging novel variant infectious bursal disease virus
文献类型： 外文期刊
作者： Wang G.;Jiang N.;Yu H.;Niu X.;Huang M.;Zhang Y.;Zhang W.;Han J.;Xu M.;Liu R.;Wu Z.;Han J.;Wang S.;Gao L.;Cui H.;Zhang Y.;Chen Y.;Gao Y.;Qi X.
作者机构：
关键词： Antigenicity;Immune escape;Neutralization;Novel variant IBDV (nVarIBDV);PDE
期刊名称： Veterinary Microbiology
ISSN： 0378-1135
年卷期： 2024 年 293 卷
页码：
收录情况： SCIE（2024版）
摘要： Infectious bursa disease (IBD) is an acute, highly contactable, lethal, immunosuppressive infectious disease caused by the Infectious bursa disease virus (IBDV). Currently, the emerged novel variant IBDV (nVarIBDV) and the sustainedly prevalent very virulent IBDV (vvIBDV) are the two most prevalent strains of IBDV in China. The antigenic properties of the two prevalent strains differed significantly, which led to the escape of nVarIBDV from the immune protection provided by the existing vvIBDV vaccine. However, the molecular basis of the nVarIBDV immune escape remains unclear. In this study, we demonstrated, for the first time, that residues 252, 254, and 256 in the PDE of VP2 are involved in the immune escape of the emerging nVarIBDV. Firstly, the IFA-mediated antigen-antibody affinity assay showed that PBC and PDE of VP2 could affect the affinity of vvIBDV antiserum to VP2, of which PDE was more significant. The key amino acids of PDE influencing the antigen-antibody affinity were also identified, with G254N being the most significant, followed by V252I and I256V. Then the mutated virus with point or combined mutations was rescued by reverse genetics. it was further demonstrated that mutations of V252I, G254N, and I256V in PDE could individually or collaboratively reduce antigen–antibody affinity and interfere with antiserum neutralization, with G254N being the most significant. This study revealed the reasons for the widespread prevalence of nVarIBDV in immunized chicken flocks and provided innovative ideas for designing novel vaccines that match the antigen of the epidemic strain. © 2024 Elsevier B.V.
分类号：
- 相关文献
作者其他论文 更多>>
-
Both yields of maize and soybean and soil carbon sequestration in typical Mollisols cropland decrease under future climate change: SPACSYS simulation
作者：Liang S.;Sun N.;Longdoz B.;Meersmans J.;Ma X.;Gao H.;Zhang X.;Qiao L.;Colinet G.;Xu M.;Wu L.
关键词：carbon sequestration efficiency;climate change;long-term fertilization;Mollisols;SOC stock;SPACSYS model;yield
-
The amino acid pattern and dynamics of body protein, body fat deposition in male and female broilers under different temperatures
作者：Xu M.;Zhao X.;Yu M.;Wang G.;Feng J.;Zhang M.
关键词：amino acid;body fat;body protein;broiler;temperature
-
Comprehensive study of non-volatile and volatile metabolites in five water lily species and varieties (Nymphaea spp.) using widely targeted metabolomics
作者：Yang G.;Wei J.;Wu Y.;Chen S.;Yu C.;Zhu Y.;Lin Z.;Lv H.;Chen Y.
关键词：
-
Anti-Biofilm Activity of Assamsaponin A, Theasaponin E1, and Theasaponin E2 against Candida albicans
作者：Chen Y.;Gao Y.;Li Y.;Yin J.
关键词：anti-biofilm activity;assamsaponin A;Camellia sinensis seeds;theasaponin E1;theasaponin E2;virulence factors
-
AwSclB regulates a network for Aspergillus westerdijkiae asexual sporulation and secondary metabolism independent of the fungal light control
作者：Wang G.;Liu Y.;Hu Y.;Pan J.;Wei Z.;Tai B.;Yang B.;Li E.;Xing F.
关键词：Asexual sporulation;Aspergillus westerdijkiae;Light control;Ochratoxin A;Secondary metabolism
-
Pan-genome and phylogenomic analyses highlight Hevea species delineation and rubber trait evolution
作者：Fang Y.;Xiao X.;Lin J.;Lin Q.;Wang J.;Liu K.;Li Z.;Xing J.;Liu Z.;Wang B.;Qi Y.;Long X.;Zeng X.;Hu Y.;Qi J.;Qin Y.;Yang J.;Zhang Y.;Zhang S.;Ye D.;Zhang J.;Liu J.;Tang C.
关键词：
-
Multi-omics analyses reveal the responses of wheat (Triticum aestivum L.) and rhizosphere bacterial community to nano(micro)plastics stress
作者：Zhuang M.;Qiao C.;Han L.;Bi Y.;Cao M.;Wang S.;Guo L.;Pang R.;Xie H.
关键词：Metabolomic;Nanoplastics;Rhizosphere soil bacteria;Transcriptome;Wheat