数字农科院2.0

Infection and resistance mechanisms of phage vB_VpM-PP270 in Vibrio parahaemolyticus via capsular polysaccharide

文献类型： 外文期刊

作者： Zhu Xihui;Hong Junshan;Xu Jiayin;Tang Luqi;Li Pei;Jiang Wei;Zhang Wei

作者机构：

关键词： Microbiology;Molecular biology

期刊名称： iScience

ISSN： 2589-0042

年卷期： 2025 年 28 卷 11 期

页码：

收录情况： SCIE（2025版）

摘要： Vibrio parahaemolyticus is a major foodborne pathogen that threatens public health and aquaculture, necessitating alternative biocontrol strategies such as phage therapy. This study introduces phage vB_VpM-PP270, isolated from aquaculture wastewater, and explores its infection mechanism targeting V. parahaemolyticus VP270. The research highlights capsular polysaccharide (CPS) as the primary receptor for phage adsorption and identifies ORF22, a tail spike protein, as the receptor-binding protein. PP270 demonstrates stability across a wide pH range and temperatures, effectively inhibiting VP270 growth in culture and on chilled shrimp. Phage-resistant mutants show mutations in CPS biosynthesis genes, which reduce phage adsorption and promote biofilm formation. These findings provide insights into phage-host interactions and offer a framework for developing phage therapies resilient to resistance, with significant implications for food safety and sustainable aquaculture practices.

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