数字农科院2.0

Molecular detection and diversity of xylanase genes in alpine tundra soil

文献类型： 外文期刊

作者： Wang, Guozeng;Wang, Yaru;Yang, Peilong;Luo, Huiying;Huang, Huoqing;Shi, Pengjun;Meng, Kun;Yao, Bin

作者机构：

关键词： Alpine tundra soil;Degenerate PCR;Gene diversity;Glycosyl hydrolase;Xylanase

期刊名称： APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Xylan is a major polysaccharide in plant cell walls, and its degradation is mainly conducted by microbial xylanases in nature. To explore the xylanase diversity in the environment, two sets of degenerate primers were designed based on the microbial xylanase sequences in Pfam database of glycosyl hydrolase (GH) family 10 and 11 and were used to amplify objective gene fragments directly from the alpine tundra soil DNA of the Tianshan Mountains, China. Ninety-six distinct GH 10 and 31 GH 11 xylanase gene fragments were retrieved, and most of them have low identities with known sequences in GenBank. Based on phylogenetic analysis, all of the GH 10 xylanase sequences fell into six clusters and were related to xylanases from Actinobacteria, Proteobacteria, Verrucomicrobia, Bacteroidetes, Firmicutes, and Acidobacteria. Three clusters of GH 11 xylanase sequences were established, and two of them were related with enzymes from fungi. These results indicated the diversity of xylanase genes in this cold environment. Four xylanolytic strains were isolated from the soil, and GH 10 xylanase gene fragments were cloned using the same primers. A full-length gene was obtained and expressed in Escherichia coli, and the recombinant enzyme showed some cold-related characteristics. Our study provides an efficient molecular approach to study xylanase in complex environments and casts an insight into the diversity and distribution of xylanases in a cold environment, which is very meaningful to understand their roles in xylan degradation in nature.

分类号： Q939.9

  • 相关文献

[1]Gene Cloning, Expression, and Characterization of a Novel Phytase from Dickeya paradisiaca. Gu, Weina,Huang, Huoqing,Meng, Kun,Yang, Peilong,Fu, Dawei,Luo, Huiying,Wang, Yaru,Yao, Bin,Gu, Weina,Zhan, Zhichun.

[2]Plant immunity suppression by an β-1,3-glucanase of the maize anthracnose pathogen Colletotrichum graminicola. Xiaoyu Gu,Zhiyan Cao,Zhiqiang Li,Haiyue Yu,Wende Liu. 2024

[3]Identification and molecular mapping of a resistance gene to powdery mildew from the synthetic wheat line M53. Zhang, Zengyan,Xin, Zhiyong,Li, Tao,Hu, Yingkao,Duan, Xiayu.

[4]Gene flow among different teosinte taxa and into the domesticated maize gene pool. Warburton, Marilyn L.,Wilkes, Garrison,Taba, S.,Dreisigacker, Susanne,Bedoya, Claudia,Prasanna, B. M.,Charcosset, Alain,Mir, Celine,Dumas, Fabrice,Madur, Delphine,Xie, C. X.,Hearne, Sarah,Franco, Jorge.

[5]Lipase Diversity in Glacier Soil Based on Analysis of Metagenomic DNA Fragments and Cell Culture. Zhang Yuhong,Shi, Pengjun,Meng, Kun,Bai, Yingguo,Wang, Guozeng,Yao, Bin,Zhang Yuhong,Liu, Wanli,Zhan, Zhichun.

[6]Characterization and identification of the xylanolytic enzymes from Aspergillus fumigatus Z5. Miao, Youzhi,Li, Juan,Shen, Qirong,Zhang, Ruifu,Zhang, Ruifu,Xiao, Zhizhuang. 2015

[7]Cloning of beta-1,3-1,4-glucanase gene from Bacillus licheniformis EGW039 (CGMCC 0635) and its expression in Escherichia coli BL21 (DE3). Teng, Da,Wang, Jian-Hua,Fan, Ying,Yang, Ya-lin,Tian, Zi-gang,Luo, Jin,Yang, Guan-pin,Zhang, Fan.

[8]Improvement of alkali stability and thermostability of Paenibacillus campinasensis Family-11 xylanase by directed evolution and site-directed mutagenesis. Zheng, Hongchen,Liu, Yihan,Han, Yang,Lu, Fuping,Zheng, Hongchen,Liu, Yihan,Sun, Mingzhe,Han, Yang,Wang, Jianling,Lu, Fuping,Zheng, Hongchen,Sun, Junshe,Liu, Yihan,Wang, Jianling,Sun, Mingzhe,Lu, Fuping.

[9]A new acidophilic endo-beta-1,4-xylanase from Penicillium oxalicum: cloning, purification, and insights into the influence of metal ions on xylanase activity. Liao, Hanpeng,Sun, Shaowei,Wang, Pan,Bi, Wenli,Wei, Zhong,Mei, Xinlan,Liu, Dongyang,Raza, Waseem,Shen, Qirong,Xu, Yangchun,Tan, Shiyong,Tan, Shiyong.

[10]Production of xylobiose from the autohydrolysis explosion liquor of corncob using Thermotoga maritima xylanase B (XynB) immobilized on nickel-chelated Eupergit C. Tan, S. S.,Li, D. Y.,Jiang, Z. Q.,Zhu, Y. P.,Shi, B.,Li, L. T..

[11]Biochemical analysis of a highly specific, pH stable xylanase gene identified from a bovine rumen-derived metagenomic library. Gong, X.,Gruniniger, R. J.,Forster, R. J.,Teather, R. M.,McAllister, T. A.,Gong, X..

[12]Structural insight into potential cold adaptation mechanism through a psychrophilic glycoside hydrolase family 10 endo-beta-1,4-xylanase. Zheng, Yingying,Li, Yujie,Liu, Weidong,Chen, Chun-Chi,He, Miao,Xu, Zhongxia,Liu, Meixia,Guo, Rey-Ting,Ma, Yanhe,Ko, Tzu-Ping,Luo, Huiying,Yao, Bin,He, Miao,Liu, Meixia,Xu, Zhongxia.

[13]Codon optimization of Bacillus licheniformis beta-1,3-1,4-glucanase gene and its expression in Pichia pastoris. Teng, Da,Fan, Ying,Yang, Ya-lin,Tian, Zi-gang,Luo, Jin,Wang, Jian-hua.

[14]A novel bifunctional GH51 exo-alpha-L-arabinofuranosidase/endo-xylanase from Alicyclobacillus sp A4 with significant biomass-degrading capacity. Yang, Wenxia,Bai, Yingguo,Yang, Peilong,Luo, Huiying,Huang, Huoqing,Meng, Kun,Shi, Pengjun,Wang, Yaru,Yao, Bin. 2015

[15]A novel cold-active xylanase gene from the environmental DNA of goat rumen contents: Direct cloning, expression and enzyme characterization. Wang, Guozeng,Luo, Huiying,Wang, Yaru,Huang, Huoqing,Shi, Pengjun,Yang, Peilong,Meng, Kun,Bai, Yingguo,Yao, Bin.

[16]Two Family 11 Xylanases from Achaetomium sp Xz-8 with High Catalytic Efficiency and Application Potentials in the Brewing Industry. Zhao, Liang,Meng, Kun,Bai, Yingguo,Shi, Pengjun,Huang, Huoqing,Luo, Huiying,Wang, Yaru,Yang, Peilong,Yao, Bin,Zhao, Liang,Song, Wei. 2013

[17]Two xylose-tolerant GH43 bifunctional beta-xylosidase/alpha-arabinosidases and one GH11 xylanase from Humicola insolens and their synergy in the degradation of xylan. Yang, Xinzhuo,Shi, Pengjun,Huang, Huoqing,Luo, Huiying,Wang, Yaru,Yao, Bin,Zhang, Wei. 2014

[18]Cloning of a New Xylanase Gene from Streptomyces sp TN119 Using a Modified Thermal Asymmetric Interlaced-PCR Specific for GC-Rich Genes and Biochemical Characterization. Zhou, Junpei,Huang, Huoqing,Meng, Kun,Shi, Pengjun,Wang, Yaru,Luo, Huiying,Yang, Peilong,Bai, Yingguo,Yao, Bin. 2010

[19]Gene Cloning, Overexpression, and Characterization of a Xylanase from Penicillium sp CGMCC 1669. Liu, Wanli,Shi, Pengjun,Yang, Peilong,Wang, Guozeng,Wang, Yaru,Luo, Huiying,Yao, Bin,Liu, Wanli,Chen, Qiang. 2010

[20]An acid and highly thermostable xylanase from Phialophora sp G5. Zhang, Fan,Shi, Pengjun,Bai, Yingguo,Luo, Huiying,Yuan, Tiezheng,Huang, Huoqing,Yang, Peilong,Yao, Bin,Zhang, Fan,Miao, Lihong. 2011

作者其他论文 更多>>