数字农科院2.0

Development of a SCAR marker by inter-simple sequence repeat for diagnosis of dwarf bunt of wheat and detection of Tilletia controversa Kuhn

文献类型： 外文期刊

作者： Gao, L.;Chen, W. Q.;Liu, T. G.

作者机构：

关键词： detection limit;genetic variation

期刊名称： FOLIA MICROBIOLOGICA

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Dwarf bunt of wheat, caused by Tilletia controversa Kuhn, is a destructive disease on wheat as well as an important internationally quarantined disease in many countries. The primer ISSR818 generated a polymorphic pattern displaying a 867-bp DNA fragment specific for T. controversa. The marker was converted into a sequence characterized amplified region (SCAR), and specific primers (TCKSF3/TCKSR3) designed for use in PCR detection assays; they amplified a unique DNA fragment in all isolates of T. controversa but not in the related pathogens. The detection limit with the primer set (TCKSF3/TCKSR3) was 5 ng of DNA which could be obtained from 5.5 mu g of teliospores in a 25-mu L PCR reaction mixture.

分类号： Q93

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