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Combined Transcriptomic Analysis and RNA Interference Reveal the Effects of Methoxyfenozide on Ecdysone Signaling Pathway of Spodoptera exigua

文献类型： 外文期刊

作者： Zhixian Zhang ; Yajie Ma ; Xiaoyan Ma ; Hongyan Hu ; Dan Wang ; Xianpeng Song ; Xiangliang Ren ; Yan Ma

关键词： Spodoptera exigua; methoxyfenozide; toxicity; ecdysone receptor A; mortality

期刊名称： INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

ISSN： 1422-0067

年卷期： 2021 年

页码：

收录情况： JCR（2021版）

摘要： Spodoptera exigua is a worldwide pest afflicting edible vegetables and has developed var‐ ying levels of resistance to insecticides. Methoxyfenozide (MET), an ecdysteroid agonist, is effective against lepidopteran pests such as S. exigua. However, the mechanism of MET to S. exigua remains unclear. In this study, we analyzed the expression patterns of genes related to the ecdysone signal‐ ing pathway in transcriptome data treated with sublethal doses of MET and analyzed how expres‐ sion levels of key genes affect the toxicity of MET on S. exigua. Our results demonstrated that 2639 genes were up‐regulated and 2512 genes were down‐regulated in S. exigua treated with LC30 of MET. Of these, 15 genes were involved in the ecdysone signaling pathway. qPCR results demonstrated that ecdysone receptor A (EcRA) expression levels significantly increased in S. exigua when treated with different doses of MET, and that the RNAi‐mediated silencing of EcRA significantly increased mortality to 55.43% at 72 h when L3 S. exigua larvae were exposed to MET at the LC30 dose. Addi‐ tionally, knocking down EcRA suppressed the most genes expressed in the ecdysone signaling path‐ way. The combination of MET and dsEcRA affected the expression of E74 and enhanced the expres‐ sion of TREA. These results demonstrate that the adverse effects of sublethal MET disturb the ecdy‐ sone signaling pathway in S. exigua, and EcRA is closely related to MET toxic effect. This study increases our collective understanding of the mechanisms of MET in insect pests.

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