数字农科院2.0

Determining the Subcellular Localization of Proteins in the Different Membranes of Diatom Secondary Plastid

文献类型： 外文期刊

作者： Xiaojuan Liu;Yangmin Gong

作者机构：

关键词： Co-localization;GFP;mRFP;Phaeodactylum tricornutum;Secondary plastid

期刊名称： Methods in molecular biology (Clifton, N.J.)

ISSN： 1940-6029

年卷期： 2024 年 2776 卷

页码：

收录情况： 无来源刊（2024版）

摘要： Diatoms such as Phaeodactylum tricornutum arose through a process termed secondary endosymbiosis, in which red alga-derived plastids are surrounded by a complicated membrane system. Subcellular marker proteins provide defined localizations on the compartmental and even sub-compartmental levels in the complex plastids of diatoms. Here we introduce how to use subcellular marker proteins and in vivo co-localization in the diatom P. tricornutum by presenting a step-by-step method allowing the determination of subcellular localization of proteins in different membranes of the secondary plastid. This chapter describes the materials required and the procedures of transformation and microscopic observation.

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