数字农科院2.0

Probiotic Potentials and Protective Effects of Ligilactobacillus animalis LA-1 Against High-Fat Diet-Induced Obesity in Mice

文献类型： 外文期刊

作者： Qingya Wang;Yuyin Huang;Kun Meng;Haiou Zhang;Yunsheng Han;Rui Zhang;Xiling Han;Guohua Liu;Hongying Cai;Peilong Yang

作者机构：

关键词： amino acid metabolism;gut microbiota;high-fat diet;Ligilactobacillus animalis LA-1;obesity;probiotic activities;SCFAs

期刊名称： Nutrients

ISSN： 2072-6643

年卷期： 2025 年 17 卷 14 期

页码：

收录情况： SCIE（2025版）

摘要： Background/Objectives: Obesity is increasingly recognized as a global health concern due to its association with metabolic disorders and gut microbiota dysbiosis. While probiotics offer promise in regulating gut microbiota and improving host metabolism, strain-specific effects remain underexplored, particularly for canine-derived probiotics. This study aimed to isolate and characterize a novel probiotic strain, Ligilactobacillus animalis LA-1, and evaluate its anti-obesity effects and underlying mechanisms using a high-fat diet (HFD)-induced obese mouse model. Methods: LA-1 was isolated from the feces of a healthy dog and assessed for probiotic potential in vitro, including gastrointestinal tolerance, bile salt hydrolase activity, cholesterol-lowering capacity, and fatty acid absorption. Male C57BL/6J mice were fed either a standard chow diet or an HFD for 16 weeks, with HFD mice receiving oral LA-1 supplementation (2 × 109 CFU/day). Multi-omics analyses, including 16S rRNA gene sequencing, short-chain fatty acid (SCFA) quantification, and untargeted liver metabolomics, were employed to investigate the effects of LA-1 on gut microbiota composition, metabolic pathways, and obesity-related phenotypes. Results: LA-1 supplementation significantly alleviated HFD-induced weight gain, hepatic lipid accumulation, and adipose tissue hypertrophy, without affecting food intake. It improved serum lipid profiles, reduced liver injury markers, and partially restored gut microbiota composition, decreasing the Firmicutes/Bacteroidetes ratio and enriching SCFA-producing genera. Total SCFA levels, particularly acetate, propionate, and butyrate, increased following LA-1 treatment. Liver metabolomics revealed that LA-1 modulated pathways involved in lipid and amino acid metabolism, resulting in decreased levels of acetyl-CoA, triglycerides, and bile acids. Conclusions: L. animalis LA-1 exerts anti-obesity effects via gut microbiota modulation, enhanced SCFA production, and hepatic metabolic reprogramming. These findings highlight its potential as a targeted probiotic intervention for obesity and metabolic disorders.

分类号：

  • 相关文献

[1]In Vitro Evaluation of Probiotic Activities and Anti-Obesity Effects of Enterococcus faecalis EF-1 in Mice Fed a High-Fat Diet. Hongying Cai,Qingya Wang,Xiling Han,Haiou Zhang,Na Wang,Yuyin Huang,Peilong Yang,Rui Zhang,Kun Meng. 2024

[2]Different Diet Energy Levels Alter Body Condition, Glucolipid Metabolism, Fecal Microbiota and Metabolites in Adult Beagle Dogs. Haoran Sun,Qiaoru Zhang,Chao Xu,Aipeng Mao,Hui Zhao,Miao Chen,Weili Sun,Guangyu Li,Tietao Zhang. 2023

[3]Highly expandable edible hydrogels for the prevention and treatment of obesity through dietary intervention. Huiyuan Yang,Haixia Yang,Chenhui Zhu,Daidi Fan,Jianjun Deng. 2023

[4]Metabolomic signatures for liver tissue and cecum contents in high-fat diet-induced obese mice based on UHPLC-Q-TOF/MS. Hongying Cai,Zhiguo Wen,Kun Meng,Peilong Yang. 2021

[5]In Vitro Fermentation of Beechwood Lignin–Carbohydrate Complexes Provides Evidence for Utilization by Gut Bacteria. Xiaochen Ma,Shujun Liu,Hongliang Wang,Yulu Wang,Zhen Li,Tianyi Gu,Yulong Li,Fengjiao Xin,Boting Wen. 2023

[6]Differential Analysis of Fecal SCFAs and Their Contribution to Adipogenesis in UCP1 Knock-In Pigs. Chengyu Zhao,Jianfei Pan,Yanfang Wang,Jianguo Zhao,Jiaojiao Huang. 2025

[7]High-fat diet-disturbed gut microbiota-colonocyte interactions contribute to dysregulating peripheral tryptophan-kynurenine metabolism. Penghao Sun,Mengli Wang,Yong Xin Liu,Luqi Li,Xuejun Chai,Wei Zheng,Shulin Chen,Xiaoyan Zhu,Shanting Zhao. 2023

[8]DHA Suppresses Hepatic Lipid Accumulation via Cyclin D1 in Zebrafish. Qianwen Ding,Qiang Hao,Qingshuang Zhang,Yalin Yang,Rolf Erik Olsen,Einar Ringø,Chao Ran,Zhen Zhang,Zhigang Zhou. 2022

[9]Targeted microbiome metabolomics reveals flaxseed oil supplementation regulated the gut microbiota and farnesoid X receptor pathway in high-fat diet mice. Chen Yang,Zhenxia Xu,Qingde Huang,Xu Wang,Fenghong Huang,Qianchun Deng. 2023

[10]Nuclease treatment enhanced the ameliorative effect of yeast culture on epidermal mucus, hepatic lipid metabolism, inflammation response and gut microbiota in high-fat diet-fed zebrafish. Yajie Zhao,Qiang Hao,Qingshuang Zhang,Yalin Yang,Chao Ran,Qiyou Xu,Chenglong Wu,Wenshu Liu,Siming Li,Zhen Zhang,Zhigang Zhou. 2022

[11]Addition of solid-state fermentation product of yeast ameliorated the effects of high-fat diet on hepatic lipid metabolism, epidermal mucus, intestine and liver health, and gut microbiota of zebrafish. Jie Li,Dongmei Xia,Xiufang Jing,Yajie Zhao,Qiang Hao,Qingshuang Zhang,Mingxu Xie,Yalin Yang,Chao Ran,Qiyou Xu,Chenglong Wu,Zhen Zhang,Zhigang Zhou. 2023

[12]Reconstituting gut microbiota-colonocyte interactions reverses diet-induced cognitive deficits: The beneficial of eucommiae cortex polysaccharides. Mengli Wang,Penghao Sun,Xuejun Chai,Yong Xin Liu,Luqi Li,Wei Zheng,Shulin Chen,Xiaoyan Zhu,Shanting Zhao. 2024

[13]Gut microbiota dysbiosis and oxidative damage in high-fat diet-induced impairment of spermatogenesis: Role of protocatechuic acid intervention. Hu, Ruizhi,Yang, Xizi,Wang, Long,Su, Dingding,He, Ziyu,Li, Jiaxing,Gong, Jiatai,Zhang, Wentao,Ma, Siqi,Shi, Mingkun,Lv, Jing,Zhang, Qianjin,Hou, De-Xing,Zhang, Hongfu,He, Jianhua,Yin, Yulong,Wang, Jun,Wu, Shusong. 2024

[14]The effects of sodium propionate on intestinal barrier function of genetically improved farmed tilapia in a high-lipid formulation. Ding, Qianwen,Hao, Qiang,Jin, Ya,Zhang, Qingshuang,Xie, Yadong,Yang, Yalin,Olsen, Rolf Erik,Ringo, Einar,Ran, Chao,Zhang, Zhen,Zhou, Zhigang. 2024

[15]Longitudinal multi-omics decipher chlorinated paraffin 52 disruption of gut-brain axis homeostasis in HFD-fed mice. Wang, Yanan,Dong, Shujun,Zhang, Qi,Zhang, Ruiyi,Zhang, Hao,Huang, Kunlun,Cheng, Nan. 2025

[16]Lactobacillus plantarum FRT4 alleviated obesity by modulating gut microbiota and liver metabolome in high-fat diet-induced obese mice. Hongying Cai,Zhiguo Wen,Lulu Zhao,Dali Yu,Kun Meng,Peilong Yang. 2022

[17]Effects and mechanisms of probiotics, prebiotics, synbiotics, and postbiotics on metabolic diseases targeting gut microbiota: A narrative review. Hang Yu Li,Dan Dan Zhou,Ren You Gan,Si Yu Huang,Cai Ning Zhao,Ao Shang,Xiao Yu Xu,Hua Bin Li. 2021

[18]Gut Microbiota-Testis Axis: FMT Mitigates High-Fat Diet-Diminished Male Fertility via Improving Systemic and Testicular Metabolome. Hao, Yanan,Feng, Yanni,Yan, Xiaowei,Chen, Liang,Ma, Xiangping,Tang, Xiangfang,Zhong, Ruqing,Sun, Zhongyi,Agarwal, Manjree,Zhang, Hongfu,Zhao, Yong. 2022

[19]A new strategy to alleviate the obesity induced by endocrine disruptors—A unique lysine metabolic pathway of nanoselenium Siraitia grosvenorii to repair gut microbiota and resist obesity. Yu Wang,Wei Sun,Sen Yan,Zhiyuan Meng,Ming Jia,Sinuo Tian,Shiran Huang,Xiaoxuan Sun,Shihang Han,Canping Pan,Jinling Diao,Qiuxia Wang,Wentao Zhu. 2023

[20]Kidney Bean Protein Prevents High-Fat and High-Fructose Diet-Induced Obesity, Cognitive Impairment, and Disruption of Gut Microbiota Composition. Chunyang Jiang,Shiyu Li,Hang Su,Nong Zhou,Yang Yao. 2024

作者其他论文 更多>>