数字农科院2.0

Rapid detection of grapevine berry inner necrosis virus using a simple reverse transcription loop-mediated amplification method

文献类型： 外文期刊

作者： Ying Zhang;Qingyun Yuan;Fang Ren;Guojun Hu;Xudong Fan;Yafeng Dong

作者机构：

关键词： Detection;Grapevine;Grapevine berry inner necrosis virus (GINV);RT-LAMP

期刊名称： Physiological and Molecular Plant Pathology

ISSN： 0885-5765

年卷期： 2024 年 132 卷

页码：

收录情况： SCIE（2024版）

摘要： To develop a simple method for the detection of grapevine berry inner necrosis (GINV) that does not depend on expensive instruments, a reverse transcription (RT) loop-mediated isothermal amplification (LAMP) assay was developed. A set of primers was used in RT-LAMP to specifically detect GINV and different GINV variants belonging to groups 1, 2 and 3. The sensitivity of the RT-LAMP was about 100 times greater than that of conventional RT-PCR. Furthermore, a rapid “pin-prick” assay without RNA extraction was developed to detect GINV in RT-LAMP. The assay performed well for the detection of GINV from different parts of field grapevine plants and micropropagated grapevine plantlets, and had a higher detection rate of GINV than RT-PCR in the tested grapevine samples.

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