数字农科院2.0

Gene cloning, expression, and characterization of a novel trehalose synthase from Arthrobacter aurescens

文献类型： 外文期刊

作者： Wu Xiuli;Ding Hongbiao;Yue Ming;Qiao Yu

作者机构：

关键词： Arthrobacter aurescens;Escherichia coli;Trehalose;Trehalose synthase

期刊名称： APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

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年卷期：

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收录情况： SCI

摘要： Trehalose synthase (TreS) is an intramolecular transglycosylase. It specially catalyzes the conversion of maltose and trehalose. In this study, a novel treS gene, which had a length of 1,797 bp and encoded 598 amino acids, was cloned from Arthrobacter aurescens CGMCC 1.1892 and expressed in Escherichia coli. Thin layer chromatography results indicated that it could catalyze the conversion between maltose and trehalose in one step. However, the ion chromatography results showed that, as a byproduct, about 13% glucose was also produced. The purified recombinant enzyme had a molecular weight of 68 kDa and showed its optimal activity at 35 °C and pH 6.5. This enzyme was not thermostable, and its activity was increased by 1 mM Mg~(2+), Mn ~(2+), and Ca~(2+) while strongly inhibited by 5 mM Cu ~(2+) and SDS.

分类号： Q939.9

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