数字农科院2.0

Lactobacillus paracasei Jlus66 relieves DSS-induced ulcerative colitis in a murine model by maintaining intestinal barrier integrity, inhibiting inflammation, and improving intestinal microbiota structure

文献类型： 外文期刊

作者： Yu, Fazheng;Wang, Xiaoxu;Ren, Honglin;Chang, Jiang;Guo, Jian;He, Zhaoqi;Shi, Ruoran;Hu, Xueyu;Jin, Yuanyuan;Lu, Shiying;Li, Yansong;Liu, Zengshan;Hu, Pan

作者机构：

关键词： Lactobacillus paracasei;Colitis;Intestinal biota;NF-kappa B;MAPK

期刊名称： EUROPEAN JOURNAL OF NUTRITION

ISSN： 1436-6207

年卷期： 2024 年

页码：

收录情况： SCIE（2024版）

摘要： PurposeUlcerative colitis (UC) is a serious health problem with increasing morbidity and prevalence worldwide. The pathogenesis of UC is complex, currently believed to be influenced by genetic factors, dysregulation of the host immune system, imbalance in the intestinal microbiota, and environmental factors. Currently, UC is typically managed using aminosalicylates, immunosuppressants, and biologics as adjunctive therapies, with the risk of relapse and development of drug resistance upon discontinuation. Therefore, further research into the pathogenesis of UC and exploration of potential treatment strategies are necessary to improve the quality of life for affected patients. According to previous studies, Lactobacillus paracasei Jlus66 (Jlus66) reduced inflammation and may help prevent or treat UC. MethodsWe used dextran sulfate sodium (DSS) to induce a mouse model of UC to assess the effect of Jlus66 on the progression of colitis. During the experiment, we monitored mouse body weight, food and water consumption, as well as rectal bleeding. Hematoxylin-eosin staining was performed to assess intestinal pathological damage. Protein imprinting and immunohistochemical methods were used to evaluate the protein levels of nuclear factor-kappa B (NF-kappa B), mitogen-activated protein kinase (MAPK), and tight junction (TJ) proteins in intestinal tissues. Fecal microbiota was analyzed based on partial 16S rRNA gene sequencing. ResultsJlus66 supplementation reduced the degree of colon tissue damage, such as colon shortening, fecal occult blood, colon epithelial damage, and weight loss. Supplementation with Jlus66 reduced DSS-induced upregulation of cytokine levels such as TNF-alpha, IL-1 beta, and IL-6 (p < 0.05). The NF-kappa B pathway and MAPK pathway were inhibited, and the expression of TJ proteins (ZO-1, Occludin, and Claudin-3) was upregulated. 16S rRNA sequencing of mouse cecal contents showed that Jlus66 effectively regulated the structure of the intestinal biota. ConclusionIn conclusion, these data indicate that Jlus66 can alter the intestinal biota and slow the progression of UC, providing new insights into potential therapeutic strategies for UC.

分类号：

  • 相关文献

[1]Recombinant TB9.8 of Mycobacterium bovis Triggers the Production of IL-12 p40 and IL-6 in RAW264.7 Macrophages via Activation of the p38, ERK, and NF-kappa B Signaling Pathways. Jia, Hong,Liu, Shuqing,Wu, Jing,Hou, Shaohua,Xin, Ting,Guo, Xiaoyu,Yuan, Weifeng,Zhang, Gaimei,Li, Ming,Zhu, Hongfei,Gao, Xintao,Qu, Hongfei,Zhu, Hongfei.

[2]Pmorf0222, a Virulence Factor in Pasteurella multocida, Activates Nuclear Factor Kappa B and Mitogen-Activated Protein Kinase via Toll-Like Receptor 1/2. Xu, Tenglin,Zheng, Yating,Liu, Benjin,Kou, Mingxing,Jiang, Qian,Liu, Jiasen,Kang, Hongtao,Yang, Mingfa,Guo, Dongchun,Qu, Liandong. 2023

[3]MAPK基因家族成员在棉花抗黄萎病中的功能分析. 翟伟卜,郑娜,张珊珊,郭安慧,张文蔚,简桂良,段江燕,齐放军. 2016

[4]小麦蛋白激酶TaMAPK2互作蛋白的筛选与验证. 于太飞,徐兆师,李盼松,陈明,李连城,张俊华,马有志. 2014

[5]肉仔鸡卫星细胞氧化应激时MAPK信号通路. 王成,武书庚,张海军,岳洪源,翟永功,齐广海. 2010

[6]松材线虫mapk基因克隆及RNAi效应分析. 王殿东,李莹,李娟,谢丙炎,陈国华. 2016

[7]石榴MAPK家族基因鉴定及其响应冷胁迫的表达分析. 陈利娜,曹尚银,唐丽颖,李好先,严琼,李松开,杨庆华,鲁振华. 2023

[8]基于网络药理学、分子对接及实验验证探讨胖大海抗PM2.5诱导急性肺损伤的作用机制. 张凡,杜一凡,邓肖舒,张祖烽,韩先磊,田薇,李秀梅,陈勉,刘飞,王楠. 2025

[9]Tylvalosin exhibits anti-inflammatory property and attenuates acute lung injury in different models possibly through suppression of NF-kappa B activation. Zhao, Zhanzhong,Tang, Xiangfang,Zhang, Minhong,Hou, Shaohua,Yuan, Weifeng,Zhang, Hongfu,Shi, Lijun,Jia, Hong,Liang, Lin,Zhao, Zhanzhong,Zhao, Xinghui,Fu, Ling,Chen, Wei,Zhang, Weijian,Lai, Zhi,Gao, Junfeng,Zhang, Keyu. 2014

[10]Influence of acrylamide on ROS, Hsp27 and NF-kappa B in bone marrow mesenchymal stem cells. Liu, Yang,Tao, Xiaoli,Mu, Yulian,Wang, Pan,Feng, Shutang,Li, Kui,Liu, Yang,Mu, Yulian,Wang, Pan,Feng, Shutang,Li, Kui,Liu, Yang,Mu, Yulian,Wang, Pan,Feng, Shutang,Li, Kui,Tao, Xiaoli.

[11]Fibroblast growth factor 21 (FGF21) ameliorates collagen-induced arthritis through modulating oxidative stress and suppressing nuclear factor-kappa B pathway. Yu, Yinhang,Li, Siming,Liu, Yaonan,Tian, Guiyou,Yuan, Qingyan,Bai, Fuliang,Wang, Wenfei,Ren, Guiping,Li, Deshan,Wang, Wenfei,Ren, Guiping,Li, Deshan,Zhang, Yu,Zhang, Zhiyi,Li, Siming. 2015

[12]Alleviative effect of selenium on inflammatory damage caused by lead via inhibiting inflammatory factors and heat shock proteins in chicken testes. Wang, Yan,Wang, Kexin,Huang, He,Teng, Xiaohua,Gu, Xianhong.

[13]Activation of gga-miR-155 by reticuloendotheliosis virus T strain and its contribution to transformation. Yao, Yongxiu,Kgosana, Lydia,Smith, Lorraine P.,Nair, Venugopal,Yao, Yongxiu,Kgosana, Lydia,Smith, Lorraine P.,Nair, Venugopal,Vasoya, Deepali,Watson, Mick,Vasoya, Deepali,Watson, Mick,Gao, Yulong,Wang, Xiaomei.

[14]Two myeloid differentiation factor 88 (MyD88) isoforms identified in ducks. Cheng, Yuqiang,Wang, Hengan,Yan, Yaxian,Sun, Jianhe,Ding, Chan.

[15]Anti-inflammatory effects of 4 '-demethylnobiletin, a major metabolite of nobiletin. Wu, Xian,Song, Mingyue,Rakariyatham, Kanyasiri,Zheng, Jinkai,Guo, Shanshan,Xiao, Hang,Zheng, Jinkai,Guo, Shanshan,Tang, Zhonghai,Zhou, Shuangde,Xiao, Hang.

[16]Mycoplasma bovis-derived lipid-associated membrane proteins activate IL-1 beta production through the NF-kappa B pathway via toll-like receptor 2 and MyD88. Wang, Yang,Liu, Suli,Li, Yuan,Wang, Qi,Shao, Jiari,Chen, Ying,Xin, Jiuqing. 2016

[17]Molecular cloning and functional characterization of a novel isoform of chicken myeloid differentiation factor 88 (MyD88). Qiu, Yafeng,Shen, Yang,Li, Xiangdong,Ding, Chan,Ma, Zhiyong.

[18]cAMP/PKA Signaling Pathway Induces Apoptosis by Inhibited NF-kappa B in Aluminum Chloride-Treated Lymphocytes In Vitro. Xu, Feibo,Wang, Jing,Cao, Zheng,Song, Miao,Fu, Yang,Li, Yanfei,Zhu, Yanzhu.

[19]Induction of ROS generation and NF-kappa B activation in MARC-145 cells by a novel porcine reproductive and respiratory syndrome virus in Southwest of China isolate. Yan, Yulin,Liu, Qian,Huang, Hui,Shao, Zhiyong,Zang, Yating,Chen, Ling,Sun, Yongke,Gao, Hong,Yan, Yulin,Xin, Aiguo. 2015

[20]Transcriptional regulation of CD4 gene expression in porcine kidney epithelial cells by virus-like double-stranded RNA and DNA methyltransferase inhibitor. Wang, X. S.,Zhai, L. W.,Wang, C. D.,Yu, Y.,Ao, H.,Bai, L. J.,He, W. Y.. 2014

作者其他论文 更多>>