数字农科院2.0

Tembusu virus induced apoptosis in vacuolate spermatogenic cells is mediated by Cytc-mediated mitochondrial apoptotic signaling pathway

文献类型： 外文期刊

作者： Huang Y.;Zhang Y.;Yang S.;Shi Y.;Chu X.;Ahmed N.;Wu J.;Chen Q.

作者机构：

关键词： Apoptosis;Cytochrome c;Duck tembusu virus;Spermatogenic cells;Testis

期刊名称： Theriogenology

ISSN： 0093-691X

年卷期： 2024 年 215 卷

页码：

收录情况： SCIE（2024版）

摘要： Duck Tembusu virus (DTMUV) is an emerging mosquito-borne flavivirus that infects mainly poultry and has caused huge economic losses to the poultry farming industry in China. Also known as duck hemorrhagic ovarian disease, DTMUV principally destroys ovarian tissue in ducks, causing a dramatic drop in egg production. and can also invade the male reproductive system causing lesions. Currently, little research has been done to reveal the underlying mechanisms of reproductive dysfunction in ducks caused by DTMUV infection. In this study, histopathological analysis and electron microscopy of testes of ducks infected with DTMUV showed that DTMUV caused testicular atrophy and cytoplasmic vacuolation in ducks. Terminal Deoxynucleotidyl Transferase-Mediated Nick-End Labeling (TUNEL) staining and real-time quantitative PCR(RT-qPCR) results further indicated that DTMUV induced spermatogenic cells apoptosis. After DTMUV infection, a large amount of cytochrome c(Cytc) was released from the mitochondrial matrix into the cytoplasm, activating downstream target proteins and causing apoptosis. To sum up, DTMUV induces spermatogenic cell apoptosis through the Cytc-induced mitochondrial apoptosis pathway, our study provides evidence for DTMUV infection-induced male reproductive disorders. © 2023 Elsevier Inc.

分类号：

  • 相关文献

[1]Duck Tembusu virus infection causes testicular atrophy. Yufei Huang,Xiaoya Chu,Yafei Zhang,Sheng Yang,Yonghong Shi,Jingxian Wu,Qiusheng Chen. 2022

[2]Duck Tembusu virus induced mitophagy in vacuolate spermatogenic cells is mediated by PINK1-Parkin pathway. Yufei Huang,Xiaoman Yan,Xiaoya Chu,Yonghong Shi,Jacob Xiang,Sheng Yang. 2025

[3]Effects of camptothecin and hydroxycamptothecin on insect cell lines Sf21 and IOZCAS-Spex-II. Zhang, Lan,Zhang, Yanning,He, Weizhi,Ma, Dejun,Jiang, Hongyun. 2012

[4]Lipopeptide induces apoptosis in fungal cells by a mitochondria-dependent pathway. Zhu Fayin,Yu Ziniu,Zhao Xiuyun,Du Peng,Yang Xiufen,Qiu Dewen,Chen Jingyuan. 2010

[5]Duck tembusu virus and its envelope protein induce programmed cell death. Shaozhou, Wulin,Li, Chenxi,Zhang, Qingshan,Meng, Runzhe,Gao, Youlan,Liu, Hongyu,Bai, Xiaofei,Chen, Yuhuan,Liu, Ming,Liu, Siguo,Zhang, Yun.

[6]Role of apoptosis in Duck Tembusu virus infection of duckling brains in vivo. Sheng Yang,Jingxian Wu,Yufei Huang,Yafei Zhang,Yonghong Shi,Ping Yang,Qiusheng Chen. 2022

[7]The effects of IGF-1 on mouse spermatogenesis using an organ culture method. Yao, Jianfei,Gao, Jun,Wang, Mingming,Li, Xiangdong,Zuo, Haiyang,Wang, Dong.

[8]Occurrence of soil insect pests: insight from classical identification supplemented with DNA barcoding. Li, K.,Yin, J.,Zhang, S.,Kajuga, J.,Hategekimana, A.,Waweru, B.,Li, H.,Li, H..

[9]Epitope Identification and Application for Diagnosis of Duck Tembusu Virus Infections in Ducks. Li, Chenxi,Shaozhou, Wulin,Bai, Xiaofei,Zhang, Qingshan,Hua, Ronghong,Liu, Ming,Zhang, Yun,Liu, Junyan,Liu, Jyung-Hurng. 2016

[10]Development of a live attenuated vaccine candidate against duck Tembusu viral disease. Li, Guoxin,Gao, Xuyuan,Xiao, Yali,Liu, Shaoqiong,Peng, Shan,Li, Xuesong,Shi, Ying,Zhang, Yuee,Yu, Lei,Wu, Xiaogang,Yan, Pixi,Yan, Liping,Teng, Qiaoyang,Tong, Guangzhi,Li, Zejun. 2014

[11]Comparison of real-time reverse transcription loop-mediated isothermal amplification and real-time reverse transcription polymerase chain reaction for duck Tembusu virus. Yan, Liping,Peng, Shan,Yan, Pixi,Zhou, Jiewen,Teng, Qiaoyang,Li, Guoxin,Li, Xuesong,Li, Zejun,Li, Zejun.

[12]An adapted duck Tembusu virus induces systemic infection and mediates antibody-dependent disease severity in mice. Liu, Zongliang,Ji, Yanhong,Huang, Xiaohui,Fu, Yuguang,Cai, Xuepeng,Zhu, Qiyun,Wei, Jianzhong.

[13]Development of a solid-phase competition ELISA to detect antibodies against newly emerged Duck Tembusu virus. Fu, Yuguang,Ji, Yanhong,Liu, Bin,Dafallah, Rihab Muhammad,Zhu, Qiyun.

[14]The vaccine efficacy of recombinant duck enteritis virus expressing secreted E with or without PrM proteins of duck tembusu virus. Chen, Pucheng,Liu, Jinxiong,Jiang, Yongping,Zhao, Yuhui,Li, Qimeng,Wu, Li,He, Xijun,Chen, Hualan.

[15]Comparison of endocytosis pathways of Duck Tembusu virus in BHK-21 and duck embryo fibroblasts. Xuemei Xian,Sheng Yang,Yonghong Shi,Qiusheng Chen. 2023

[16]Ultrastructural study of the duck brain infected with duck Tembusu virus. Sheng Yang,Yonghong Shi,Jingxian Wu,Qiusheng Chen. 2023

[17]A Recombinant Adenovirus Expressing The E. Protein Of Duck T embusu Virus Induces Protective Immunity In Duck. Tang, JY, Yin, DD, Wang, R, Zhou, Q, Zhou, XY, Xing, X, Liu, HM, Liu, GQ, Wang, GJ. 2019

[18]Development and validation of one-step SYBR green real-time RT-PCR for the rapid detection of newly emerged duck Tembusu virus. Liu, Zongliang,Fu, Yuguang,Ji, Yanhong,Cai, Xuepeng,Zhu, Qiyun,Wei, Jianzhong. 2013

[19]Interleukin-2 Shows High Adjuvanticity For An Inactivated Vaccine Against Duck Tembusu Virus Disease. Gao, XT, Ren, X, Zhang, S, Song, HZ, Guo, XY, Jia, H, Xin, T, Jiang, YT, Zhang, ZF, Hou, SH. 2020

[20]Repertoire of porcine MicroRNAs in adult ovary and testis by deep sequencing. Chen, Haosi,Wang, Xin,Gao, Xiaolian,Li, Mingzhou,Liu, Yingkai,Wang, Tao,Guan, Jiuqiang,Luo, Zonggang,Ma, Jideng,Mu, Zhiping,Jiang, An-an,Zhu, Li,Li, Xuewei,Chen, Lei,Wang, Jinyong,Lang, Qiulei,Zhou, Xiaochuan,Zeng, Wenxian,Li, Ning,Li, Kui.

作者其他论文 更多>>