数字农科院2.0

K404 Acetylation of Glutamate Dehydrogenase CrGdh2 Is Necessary for Mycoparasitism of Clonostachys chloroleuca against Sclerotinia sclerotiorum

文献类型： 外文期刊

作者： Yang, Yufeng;Lv, Binna;Yang, Qingyun;Baima, Zhuoga;Li, Shidong;Lu, Xiaohong;Sun, Manhong

作者机构：

关键词： Clonostachys chloroleuca;mycoparasitism;acetylation modification;glutamate dehydrogenase;site-directed mutagenesis

期刊名称： JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY

ISSN： 0021-8561

年卷期： 2026 年

页码：

收录情况： SCIE（2025版） ; ; EI（2025版）

摘要： Clonostachys chloroleuca is a potential biocontrol fungus capable of parasitizing various plant fungal pathogens. In the highly efficient strain 67-1, we identified a glutamate dehydrogenase gene, CrGdh2, during mycoparasitization of Sclerotinia sclerotiorum. CrGdh2 is essential for the biocontrol activity of C. chloroleuca, as its deletion compromised control efficacy against Sclerotinia rot and tomato root rot by 27.9% and 39.8%, respectively, compared to the wild-type strain. Acetylated CrGdh2 was investigated using acetylation and deacetylation mimetic mutants. The results showed that when strains were inoculated on S. sclerotiorum sclerotia, no obvious infection structures were detected in Delta CrGdh2 and Delta CrGdh2-CK404R. In pot experiments, both mutants also showed over 30% reduction in biocontrol efficacy, in contrast to the wild-type and Delta CrGdh2-CK404Q strains, indicating the essential role of K404 acetylation. The findings reveal the roles of glutamate dehydrogenase and its acetylation in mycoparasitism, providing novel insight into the biocontrol mechanisms of C. chloroleuca.

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