数字农科院2.0

Joint analysis of transcriptome and metabolome reveals some genes and metabolomic variations involved in postharvest stress response of Vitis vinifera to Aspergillus westerdijkiae

文献类型： 外文期刊

作者： Yafan Hu;Gang Wang;Xiaoyu Tian;Yibing Liu;Weizhao Li;Fuguo Xing

作者机构：

关键词： Aspergillus westerdijkiae;Postharvest infection;Trans-3-Indoleacrylic acid;Vitis vinifera;β-glucosidase

期刊名称： Postharvest Biology and Technology

ISSN： 0925-5214

年卷期： 2025 年 231 卷

页码：

收录情况： SCIE（2025版）

摘要： Postharvest fungal infection poses significant threats to grapes quality and food safety, with Aspergillus westerdijkiae emerging as a producer of ochratoxin. This study integrated the transcriptomics and metabolomics of interaction between Vitis vinifera and A. westerdijkiae during postharvest infection based on artificial inoculation. The growth of A. westerdijkiae in grapes was significantly decreased, and the genes in ochratoxin synthesis cluster were downregulated. Transcriptomic analysis in grapes indicated that the expressions of CES9 involved in cellulose synthesis, CSLG3 and CSLD4 in hemicellulose synthesis, and PAE10 and GAE3 in pectin synthesis were all upregulated. Additionally, PAL, 4CL1, 4CL9, and CAD1 were upregulated, promoting lignin synthesis. The increased expressions of these pathways in grapes collectively suggest the enhancing defenses against A. westerdijkiae. MYB and MRKY family genes were also significantly altered by A. westerdijkiae infection. Metabolomic analysis revealed a marked increase in trans-3-indoleacrylic acid (IDA) which showed strong positive correlations with multiple defense-related genes, suggesting IDA might be involved in fungi-plant interactions. Additionally, knocking out the AoFc_150 gene encoding a β-glucosidase led to a significant decrease in β-glucosidase activity and reduction of infestation capacity of A. westerdijkiae to grapes. In conclusion, these findings advance our understanding of the interactions between V. vinifera and A. westerdijkiae, offering valuable insights for improving postharvest quality and controlling fungal infections in grapes.

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