数字农科院2.0

High plasticity of ribosomal DNA organization in budding yeast

文献类型： 外文期刊

作者： Shuangying Jiang;Zelin Cai;Yun Wang;Cheng Zeng;Jiaying Zhang;Wenfei Yu;Chenghao Su;Shijun Zhao;Ying Chen;Yue Shen;Yingxin Ma;Yizhi Cai;Junbiao Dai

作者机构：

关键词： （0-1-7）3D genome organization;CP: Molecular biology;genome plasticity;genome simplification;loxPsym site;nucleolus structure;rDNA copy number;ribosomal DNA

期刊名称： Cell Reports

ISSN： 2211-1247

年卷期： 2024 年 43 卷 2 期

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收录情况： SCIE（2024版）

摘要： In eukaryotic genomes, rDNA generally resides as a highly repetitive and dynamic structure, making it difficult to study. Here, a synthetic rDNA array on chromosome III in budding yeast was constructed to serve as the sole source of rRNA. Utilizing the loxPsym site within each rDNA repeat and the Cre recombinase, we were able to reduce the copy number to as few as eight copies. Additionally, we constructed strains with two or three rDNA arrays and found that the presence of multiple arrays did not affect the formation of a single nucleolus. Although alteration of the position and number of rDNA arrays did impact the three-dimensional genome structure, the additional rDNA arrays had no deleterious influence on cell growth or transcriptomes. Overall, this study sheds light on the high plasticity of rDNA organization and opens up opportunities for future rDNA engineering.

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