数字农科院2.0

Enhancing the synthesis efficiency of galacto-oligosaccharides of a β-galactosidase from Paenibacillus barengoltzii by engineering the active and distal sites

文献类型： 外文期刊

作者： Haiyan Yu;Yulu Wang;Zhisen Yang;Jiabao Ying;关菲菲;Bolin Liu;Miao Miao;Abeer Mohamed;Xue Wei;Yuji Yang;Xin Liu;Linfeng Sun;Zhengqiang Jiang;Shaoqing Yang;Fengjiao Xin

作者机构：

关键词： Catalytic mechanism;Cryo-EM structure;GOS synthesis;Machine learning;β-Galactosidase

期刊名称： Food Chemistry

ISSN： 0308-8146

年卷期： 2025 年 483 卷

页码：

收录情况： SCIE（2025版） ; ; EI（2025版）

摘要： Previously, a glycoside hydrolase (GH) family 2 β-galactosidase (PbBGal2A) from Paenibacillus barengoltzii is characterized for its high transglycosylation capability. Here, the cryo-electron microscopy (cryo-EM) structure of PbBGal2A was determined, revealing an enlarged acidic catalytic pocket that facilitate the binding of carbohydrate substrates. Three structure-based strategies as well as machine learning MECE platform (method for enhancing the catalytic efficiency) were employed to identify active and distal mutations with enhanced galacto-oligosaccharides (GOS) synthesis and their synergistic effects were evaluated. The best H331V mutation yielded a maximum GOS production of 76.57 % at 4 h when 35 % (w/v) of lactose was used as a substrate. Molecular dynamics (MD) simulation analysis further indicated that distal mutations increase the rigidity of the loops surrounding the catalytic pocket. This research sheds light on the structural and catalytic mechanisms of PbBGal2A, highlighting the importance of both active and distal mutations in the efficient design of customized β-galactosidases.

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