数字农科院2.0

Protein kinase ATR inhibits E3 ubiquitin ligase CRL4PRL1 to stabilize ribonucleotide reductase in response to replication stress

文献类型： 外文期刊

作者： Weiyi Bao;Weijia Zhang;Yongchi Huang;Yan Zhao;Cong Wu;Leilei Duan;Lili Wang;Shunping Yan

作者机构：

关键词： ATR;CP: Molecular biology;DNA damage response;DNA replication stress;PRL1;ribonucleotide reductase;TSO2;WEE1

期刊名称： Cell Reports

ISSN： 2211-1247

年卷期： 2023 年 42 卷 7 期

页码：

收录情况： SCIE（2023版）

摘要： The protein kinase ATR is essential for replication stress responses in all eukaryotes. Ribonucleotide reductase (RNR) catalyzes the formation of deoxyribonucleotide (dNTP), the universal building block for DNA replication and repair. However, the relationship between ATR and RNR is not well understood. Here, we show that ATR promotes the protein stability of RNR in Arabidopsis. Through an activation tagging-based genetic screen, we found that overexpression of TSO2, a small subunit of RNR, partially suppresses the hypersensitivity of the atr mutant to replication stress. Biochemically, TSO2 interacts with PRL1, a central subunit of the Cullin4-based E3 ubiquitin ligase CRL4PRL1, which polyubiquitinates TSO2 and promotes its degradation. ATR inhibits CRL4PRL1 to attenuate TSO2 degradation. Our work provides an important insight into the replication stress responses and a post-translational regulatory mechanism for RNR. Given the evolutionary conservation of the proteins involved, the ATR-PRL1-RNR module may act across eukaryotes.

分类号：

  • 相关文献

[1]edsl、atr/nrcl、ahl19三个抗病相关的防卫基因的研究进展. 齐希梁,程红梅. 2013

[2]Identification of Fungal Strain by Fourier Transform Infrared Spectroscopy and Cluster Analysis. Chai A Li,Li Jin Ping,Shi Yan Xia,Xie Xue-wen,Li Bao-ju. 2010

[3]Targeting of the cellular ATM rather than ATR kinase exhibits therapeutic potential during the lumpy skin disease virus infection in vivo and in vitro. Shanhui Ren,Haotai Chen,Shasha Wang,Zaib Ur Rehman,Xiaolong Gao,Xue Yang,Xiangwei Wang,Xiangping Yin,Jianlin Han,Yuefeng Sun. 2025

[4]The OsbHLH002/OsICE1-OSH1 module orchestrates secondary cell wall formation in rice. Ying Chen,Haoyue Qi,Lijia Yang,Liang Xu,Jiaxuan Wang,Jiazhuo Guo,Liang Zhang,Yuanyuan Tan,Ronghui Pan,Qingyao Shu,Qian Qian,Shiyong Song. 2023

[5]Haplotype mapping of H3K27me3-associated chromatin interactions defines topological regulation of gene silencing in rice. Weizhi Ouyang,Xiwen Zhang,Minrong Guo,Jing Wang,Xiaoting Wang,Runxin Gao,Meng Ma,Xu Xiang,Shiping Luan,Feng Xing,Zhilin Cao,Jiapei Yan,Guoliang Li,Xingwang Li. 2023

[6]Control of grain size in rice by TGW3 phosphorylation of OsIAA10 through potentiation of OsIAA10-OsARF4-mediated auxin signaling. Ming Ma,Shao Yan Shen,Chen Bai,Wei Qing Wang,Xiao Hui Feng,Jie Zheng Ying,Xian Jun Song. 2023

[7]High plasticity of ribosomal DNA organization in budding yeast. Shuangying Jiang,Zelin Cai,Yun Wang,Cheng Zeng,Jiaying Zhang,Wenfei Yu,Chenghao Su,Shijun Zhao,Ying Chen,Yue Shen,Yingxin Ma,Yizhi Cai,Junbiao Dai. 2024

[8]A fungal chromatin remodeler drives transcription by establishing an open chromatin architecture and activated histone environment. Mengting Xu,Qi Zhang,Yuan Li,Wei Zhou,Lingyun Lei,Fucheng Lin,Yanjun Kou,Zeng Tao. 2025

[9]A supergene controls facultative diapause in the crop pest Helicoverpa armigera. Minghui Jin,Yan Peng,Jie Peng,Songmiao Yu,Chao Wu,Xianming Yang,Jingyun Zhu,Oscar Infante,Qi Xu,Hongru Wang,Kongming Wu,Yutao Xiao. 2024

作者其他论文 更多>>