数字农科院2.0

Epidemiological Investigation and Phylogenetic Analysis of Major Blood-Derived Pathogens in Sheep from Gansu Province

文献类型： 外文期刊

作者： Jin Luo;Li Ma;Fangyu Xiao;Muhammad Kashif Obaid;Hongfei Zheng;Qiaoyun Ren;Guiquan Guan;Hong Yin;Ping Liu

作者机构：

关键词： blood-borne pathogens;epidemiological investigation;Gansu province;phylogenetic analysis;sheep

期刊名称： Pathogens

ISSN：

年卷期： 2026 年 15 卷 1 期

页码：

收录情况： SCIE（2025版）

摘要： Investigating the prevalence and molecular genetic characteristics of Anaplasma ovis, Theileria spp., Anaplasma phagocytophilum, and hemotropic Mycoplasma infections in sheep populations across different regions of Gansu Province is of significant importance for the prevention and control of these pathogens. A total of 1523 sheep blood samples were collected from 19 counties (districts) in Gansu Province. Pathogen screening was conducted using PCR-based molecular detection techniques, followed by sequencing and phylogenetic analysis of specific genes (e.g., Msp4, 18S rRNA) from selected positive samples. Blood-borne pathogens infections in Gansu Province were widespread but unevenly distributed geographically. Theileria spp. and Anaplasma ovis were the dominant pathogens, with overall infection rates of approximately 16.7% and 9.6%, respectively. The highest Anaplasma ovis infection rate (82.5%) was observed in the Gannan region, where co-infections were common (24/97). An exceptionally high Theileria spp. infection rate (87.5%) was detected in the Zagana area. No pathogens were detected in Wuwei, Jingyuan, Huining, Jingtai, Qinghuan, or Maqu. Phylogenetic analysis revealed that the Msp4 gene sequences of Anaplasma ovis isolates from Gansu shared 99.48% homology with strains from Europe, Asia, and Africa. Anaplasma phagocytophilum isolates also showed high homology (99.53–99.84%) with multiple global strains. Seasonal data indicated significantly higher Theileria spp. infection rates in spring (23–34%) compared to other seasons (approximately 12%). Gansu Province is an endemic area for multiple blood-borne pathogens, with distinct regional clustering and seasonality in prevalence. The high conservation of pathogen gene sequences suggests genetic stability. This study provides essential epidemiological baseline data and a scientific foundation for targeted prevention and control of blood-borne pathogen diseases in sheep in Gansu Province.

分类号：

  • 相关文献

[1]Prevalences and characteristics of Trichuris spp. infection in sheep in pastoral areas of the Tianshan, Xinjiang, China. Wang, Lixia,Zhang, Guowu,Fu, Yuhang,Ning, Chengcheng,Li, Zhiyuan,Wang, Huisheng,Zhang, Jinsheng,Shang, Yunxia,Sun, Yaoqiang,Huang, Xiaoxing,Cai, Xuepeng,Xia, Xianzhu,Meng, Qingling,Qiao, Jun. 2022

[2]Genome phylogenetic analysis of Brucella melitensis in Northwest China. Cao, Xiaoan,Liu, Ping,Wu, Jinyan,Liu, Zhijie,Zhang, Yuling,Yin, Cai,Ying, Lan,Ma, Jinrui,He, Jijun,Shang, Youjun,Du, Rui,Liu, Zhiguo,Li, Zhenjun. 2025

[3]赖氨酸对绵羊GHR基因表达的影响(英文). 李建升,程胜利,韩向敏,冯瑞林,岳耀敬,杨博辉,刘建斌. 2009

[4]基于ssRNA-seq研究差异基因、NATs对藏羊高原低氧适应的调控. 岳耀敬,冯瑞林,王天翔,郭婷婷,李桂英,孙晓萍,刘建斌,李文辉,牛春娥,郭健,李范文,杨博辉. 2015

[5]Monitoring wind farms occupying grasslands based on remote-sensing data from China's GF-2 HD satellite-A case study of Jiuquan city, Gansu province, China. Shen, Ge,Xu, Bin,Chen, Shi,Zhang, Wenbo,Guo, Jian,Liu, Hang,Zhang, Yujing,Yang, Xiuchun,Jin, Yunxiang.

[6]Genotyping of ticks: first molecular report of Hyalomma asiaticum and molecular detection of tick-borne bacteria in ticks and blood from Khyber Pakhtunkhwa, Pakistan. Muhammad Kashif Obaid,Shehla Shehla,Guiquan Guan,Muhammad Rashid,Sumaira Shams. 2024

[7]Development of a novel protein chip for the detection of bluetongue virus in China. Xu, Q. Y.,Sun, E. C.,Li, J. P.,Lv, S.,Zhang, Q.,Wang, H. X.,Zhang, J. K.,Wu, D. L.,Feng, Y. F..

[8]A universal ELISA assay for detecting six strains of ovine Babesia species in China. Jianlin Xu,Jinming Wang,Zhi Li,Xin He,Shuaiyang Zhao,Quanying Ma,Xuan Li,Junlong Liu,Aihong Liu,Youquan Li,Hong Yin,Jianxun Luo,Guiquan Guan. 2021

[9]Prevalence and molecular epidemiology of the novel equine parasite Theileria haneyi in China. Yang, Guangpu,Chen, Yongyan,Chen, Kewei,Hu, Zhe,Li, Jingkun,Wang, Jingfei,Guo, Wei,Wang, Xiaojun,Du, Cheng. 2025

[10]Construction and immunological evaluation of recombinant adenovirus vaccines of new novel NADC34-PRRSV strains in pigs. Chang Zhan Xie,Ping Zhang,Zheng Wang,Yi Mo Tao,Zhuo Dong Cui,Fu Long Nan,Fu Chao Zhang,Yun Xin Ren,He Zhang,Hui Jun Lu. 2024

[11]Metabolizable protein requirements of Dorper crossbred ram lambs. Deng, K. D.,Ma, T.,Jiang, C. G.,Tu, Y.,Zhang, N. F.,Liu, J.,Zhao, Y. G.,Xu, G. S.,Diao, Q. Y.,Deng, K. D..

[12]Association between melatonin receptor 1A gene and expression of reproductive seasonality in sheep. Chu, MX,Cheng, DX,Liu, WZ,Fang, L,Ye, SC. 2006

[13]Long-term effects of ensiled cornstalk diet on methane emission, rumen fermentation, methanogenesis and weight gain in sheep. Liu, C.,Zhu, Z. P.,Shang, B.,Chen, Y. X.,Guo, T. J.,Luo, Y. M.. 2013

[14]Comparison of muscle characteristics and underpinning mechanisms between Texel and Ujumqin sheep aged from day 70 to 135 of gestation. Li, Li,Wang, Linjie,Zhong, Tao,Zhang, Hongping,Su, Hongwei,Liu, Wenzhong,Wei, Caihong,Xu, Lingyang,Zhao, Fuping,Zhang, Li,Du, Lixin,Ren, Hangxing.

[15]Study on Influences of Enzymolysis and Organic Solvent Extraction on Determination of 2 Kinds of beta(2)-Agonist Residues in Sheep Plasma and Urine. Li Yang,Su Xiao-Ou,Zhang Wei,Fan Xia,Wang Pei-Long,Wang Rui-Guo,Wang Xiao. 2014

[16]Characterization and comparative profiling of ovarian microRNAs during ovine anestrus and the breeding season. Di, Ran,He, Jianning,Liu, Qiuyue,Cao, Guiling,Wang, Jinxin,Yang, Zhimin,Ge, Ying,Chu, Mingxing,Song, Shuhui,Tian, Dongmei,Sun, Min,Zhao, Wenming,Liang, Xiaojun,Ma, Qing,Wang, Jiandong. 2014

[17]Association between polymorphism of GPR54 gene and litter size in Small Tail Han sheep. Tang, Q. Q.,Chu, M. X.,Cao, G. L.,Fang, L.,Di, R.,Feng, T.,Huang, D. W.,Li, N.. 2012

[18]The effects of dipeptidase inhibitor on peptide breakdown and VFA concentrations in rumen of sheep. Wang, Z. H.,Li, F. C.,Zhang, N. F.,Diao, Q. Y.. 2007

[19]Analysis of geographic and pairwise distances among sheep populations. Liu, J. B.,Yue, Y. J.,Lang, X.,Guo, J.,Feng, R. L.,Guo, T. T.,Yang, B. H.,Sun, X. P.,Wang, F.,Zha, X.. 2014

[20]Effect of several supplemental Chinese herbs additives on rumen fermentation, antioxidant function and nutrient digestibility in sheep. Qiao, G. H.,Zhou, X. H.,Li, Y.,Zhang, H. S.,Li, J. H.,Wang, C. M.,Lu, Y.. 2012

作者其他论文 更多>>