数字农科院2.0

Comparison of the gut microbiota composition between asymptomatic and diseased Epinephelus moara female x Epinephelus lanceolatus male with nervous necrosis virus infection

文献类型： 外文期刊

作者： Wang, Linna;Tian, Yongsheng;Li, Zhentong;Li, Ziqi;Chen, Shuai;Li, Linlin;Li, Wensheng;Wang, Qingbin;Lin, Haowei;Li, Bo

作者机构：

关键词： 16S rRNA;asymptomatic;gut microbiota;nervous necrosis virus;yunlong grouper

期刊名称： AQUACULTURE RESEARCH

ISSN： 1355-557X

年卷期： 2022 年 53 卷 2 期

页码：

收录情况： SCIE（2022版）

摘要： Yunlong grouper (Epinephelus moara female x Epinephelus lanceolatus male) is a new hybrid grouper with high economic value because it has shown some superiority in terms of growth, temperature tolerance, deformity resistance and some nutritional indicators compared with its parents. Currently, the aquaculture industry of grouper is plagued by viral diseases, and there is still no effective control method. Gut microbiomes have a significant impact on the digestion, immunity, metabolism, health and behaviours. In this study, 16S rRNA gene sequencing was performed to characterize the gut microbiota of yunlong grouper, and the differences in community composition between the asymptomatic and diseased fish naturally infected with nervous necrosis virus (NNV) were compared. A total of 3262 OTUs with >= 97% sequence similarity of 31 bacterial phyla were identified. In all grouper samples, the most abundant phylum was Proteobacteria and the most abundant genus was Blastopirellula, the predicted functions in gut bacteria were mainly involved with metabolism. There were no significant differences between two groups of yunlong grouper in alpha-diversity analysis. Specific bacterial taxa difference for each group from the phyla to the genus level was identified using LEfSe analysis. This is the first report of comparison of the gut microbiota between asymptomatic and diseased yunlong grouper under NNV infection. These findings provide an understanding about the interaction between intestinal microbiota and host under NNV infection, and provide targets for future probiotics screening.

分类号：

  • 相关文献

[1]Characterization of Gut Microbiota Compositions along the Intestinal Tract in CD163/pAPN Double Knockout Piglets and Their Potential Roles in Iron Absorption. Zhang, Xiu-Ling,Zhou, Yan-Rong,Xu, Song-Song,Xu, Si,Xiong, Yu-Jian,Xu, Kui,Xu, Chang-Jiang,Che, Jing-Jing,Huang, Lei,Liu, Zhi-Guo,Wang, Bing-Yuan,Mu, Yu-Lian,Xiao, Shao-Bo,Li, Kui. 2023

[2]甘肃地区牛源金黄色葡萄球菌分子鉴定及RAPD分型. 邓海平,蒲万霞,梁剑平,倪春霞,孟晓琴. 2011

[3]人体肠道微生物多样性和功能研究进展. 刘开朗,王加启,卜登攀,赵圣国. 2009

[4]一株溶原性鸡大肠杆菌的分子特征鉴定. 李鹤,李兆利,刘思国,倪宏波. 2016

[5]牛支原体的分离鉴定及16S rRNA基因序列分析. 伍晓红,储岳峰,张轩,赵萍,高鹏程. 2012

[6]山羊支原体山羊肺炎亚种甘肃株的分离及鉴定. 郭晗,储岳峰,赵萍,高鹏程,贺英,樊祜卿,冯小明,逯忠新. 2011

[7]DGGE法在盛夏习酒酒醅的微生物菌群结构解析中的应用. 施思,邓宇,李波,李中泰,唐云容,毛晓红,张文学. 2010

[8]绵羊体表脓包病原的分离·鉴定及药敏试验. 张晓,刘志杰,杨吉飞,陈泽,关贵全,刘爱红,任巧云,罗建勋,殷宏,李有全. 2014

[9]瘤胃古菌的多样性研究进展. 刘开朗,王加启,卜登攀,李旦. 2010

[10]茶尺蠖和灰茶尺蠖内共生菌Wolbachia的分子检测及序列分析. 周孝贵,付建玉,刘守安,毛腾飞,肖强,陈学新. 2016

[11]新疆石河子地区犬源立克次体病流行病学调查. 李重阳,孟庆玲,乔军,伍晔晖,王立霞,郭晶,马帅,才学鹏. 2018

[12]牛源荚膜血清型A型多杀性巴氏杆菌的分离鉴定及其种特异性基因的序列分析. 阚威,王谢忠,李兆才,蔡金山,王文勇,马艳萍,韩蓉,周继章,王云平. 2019

[13]不同来源可溶性有机质对稻田土壤中砷甲基化的影响. 田腾,颜蒙蒙,曾希柏,王济,白玲玉,吴翠霞,苏世鸣. 2020

[14]SPF鸡不同生长阶段粪便菌群组成及多样性研究. 周妍,刁晨曦,张圆圆,于海波,陆涛峰,赵丽丽,陈洪岩. 2017

[15]植原体分类鉴定研究进展. 杨毅,姜蕾,李世访. 2020

[16]犬粪肠球菌的分离鉴定及耐药性分析. 王俊书,徐进强,金红岩,封家旺,侯绍华. 2019

[17]牦牛漫游球菌的分离与生物学特性鉴定. 温峰琴,项海涛,郝宝成,邢小勇,包世俊,胡永浩. 2019

[18]牛病毒性腹泻病毒、宋内氏痢疾杆菌混合感染致仔鹿腹泻的研究. 乔连江,张萍,程悦宁,刘泽余,张海威,周玉成,周曼丽,徐莉,侯庆丰,杨艳玲. 2019

[19]梅花鹿瘤胃液中芽孢杆菌的分离鉴定与系统发育树构建. 刘晓颖,王卓,刘晗璐,鲍坤,李光玉. 2019

[20]转mapk双链RNA干扰表达载体黄瓜对根际土壤细菌多样性的影响. 陈国华,弭宝彬,李莹,李春月. 2013

作者其他论文 更多>>