数字农科院2.0

Quantitative lipidomics reveals the changes of lipids and antioxidant capacity in egg yolk from laying hens with fatty liver hemorrhagic syndrome

文献类型： 外文期刊

作者： Honglei Guo;Xinbo Zhang;Manhua You;Youming Shen;Shaobo Zhang;Jiefeng Li;Xin He;Xinghua Zhao;Ning Ma

作者机构：

关键词： egg quality;egg yolk;fatty liver hemorrhagic syndrome;lipid oxidation;lipidomic

期刊名称： Poultry Science

ISSN： 0032-5791

年卷期： 2024 年 103 卷 7 期

页码：

收录情况： SCIE（2024版）

摘要： In laying hens, fatty liver hemorrhagic syndrome (FLHS) is a common metabolic disorder, which can affect egg production and nutritional value. However, the impact of FLHS on the lipid content in egg yolks was not clear. In this study, FLHS model was induced by using high-energy low-protein diet, and the egg quality was evaluated. Egg yolk lipids were quantitatively analyzed by using ultra-performance liquid chromatography-mass spectrometry combined with multivariate statistical analysis. Gene expressions of the lipoprotein were determined by qRT-PCR and antioxidant capacity of the egg yolk were determined by kits. The elevated blood lipids and extensive lipid droplets observed indicated successful establishment of the FLHS model in laying hens. Measurements of egg quality showed that egg yolk weight was increased in the FLHS group. Lipidomics revealed that 1,401 lipids, comprising 27 lipid subclasses in the egg yolk. According to score plots of principal component analysis and orthogonal partial least squares discriminant analysis, different lipid profile was observed between the control and FLHS groups. A total of 97 different lipid species were screen out. Sphingolipid and glycerophospholipid metabolism were identified as key pathways. Free polyunsaturated fatty acids (PUFA) exhibited an increase in the FLHS group (P < 0.05). Notably, the form of PUFAs was changed that the FLHS group showed an increase in triacylglycerol-docosahexenoic acid and triacylglycerol-arachidonic acid in the egg yolk, while triacylglycerol-α-linolenic acid was decreased (P < 0.05). Total superoxide dismutase was decreased in the egg yolks affected by FLHS. Gene expressions of vitellogenin 2 (VTG2), VTG3, very low-density apolipoprotein II and apolipoprotein B were increased in the liver of laying hens with FLHS (P < 0.05). In conclusion, FLHS promoted the lipid transport from the liver to the yolk by upregulating lipoprotein expression, which altered lipid profile, and reduced antioxidant capacity in the yolk. This study provided a foundation for understanding the changes in lipids, lipid transport and lipid antioxidation capacity in egg yolk from laying hens with FLHS.

分类号：

  • 相关文献

[1]Association of lipidome evolution with the corresponding volatile characteristics of postmortem lamb during chilled storage. Le Xu,Chunyou Liu,Shaobo Li,Jinrong Xu,Huan Liu,Xiaochun Zheng,Dequan Zhang,Li Chen. 2023

[2]Enrichment efficiency of lutein in eggs and its function in improving fatty liver hemorrhagic syndrome in aged laying hens. Dansou D.M.,Chen H.,Yu Y.,Yang Y.,Tchana I.N.,Zhao L.,Tang C.,Zhao Q.,Qin Y.,Zhang J.. 2024

[3]Metabolite-mediated crosstalk: unraveling the interactions between gut microbiota and host in fatty liver hemorrhagic syndrome of laying hens. Shaobo Zhang,Xinghua Zhao,Xin He,Wanyu Shi,Ning Ma. 2026

[4]Astaxanthin improved the storage stability of docosahexaenoic acid-enriched eggs by inhibiting oxidation of non-esterified poly-unsaturated fatty acids. Hao Wang,Weizhao He,Dieudonné Mahukpégo Dansou,Huiyan Zhang,Ramdhan Dwi Nugroho,Chaohua Tang,Xiaoqing Guo,Yanan Yu,Qingyu Zhao,Yuchang Qin,Junmin Zhang. 2022

[5]Division of Labor among Worker Bees Is Associated with the Lipidomic Plasticity in Their Brains. Xiaojing Zhang,Yue Hao,Qingsheng Niu,Yanping Chen,Zhenyu Xia,Zihan Xie,Yazhou Zhao,Lingjie Kong,Wenjun Peng. 2022

[6]Mild methylenetetrahydrofolate reductase deficiency accelerates liver triacylglycerol and uric acid accumulation in fructose-fed mice. Dandan Sun,Xueming Zhang,Chaohua Tang,Qingyu Zhao,Yuchang Qin,Junmin Zhang. 2022

[7]Metabolic adaptations underlying evolution of Mycoplasma: Nucleotide metabolism and lipid modulation mechanisms driving growth and membrane fluidity in Mycoplasma bovis. Zhangcheng Li,Huafang Hao,Wenjing Cui,Shengli Chen,Ying Zhang,Rui Chen,Ahmed Adel Baz,Shanyu Jin,Xueyan Wang,Xinmin Yan,Pengcheng Gao,Lihua Xu,Shimei Lan,Yuefeng Chu. 2025

[8]The authentication of Yanchi tan lamb based on lipidomic combined with particle swarm optimization-back propagation neural network. Qi Yang,Dequan Zhang,Chongxin Liu,Le Xu,Shaobo Li,Xiaochun Zheng,Li Chen. 2024

[9]Random Forest-Assisted Widely Targeted Lipidomic Reveals Differences in Tan Lamb Meat Quality in Different Regions. Qi Yang,Chongxin Liu,Muxuan Xu,Minghui Gu,Le Xu,Shaobo Li,Xiaochun Zheng,Dequan Zhang,Li Chen. 2025

[10]Dietary choline and phospholipid supplementation enhanced docosahexaenoic acid enrichment in egg yolk of laying hens fed a 2% Schizochytrium powder-added diet. Wang, H.,Xu, L.,Wang, H.,Zhang, H. J.,Wang, X. C.,Wu, S. G.,Wang, J.,Qi, G. H..

[11]Proteome analysis of egg yolk after exposure to zinc oxide nanoparticles. Zhang, Pengfei,Zhao, Yong,Yu, Shuai,Hao, Yanan,Ge, Wei,Min, Lingjing,Shen, Wei,Li, Lan,Liu, Jing,Zhang, Honfu,Li, Qunjie,Kou, Xin,Ma, Huanfa.

[12]Development and application of a SFC–DAD–MS/MS method to determine carotenoids and vitamin A in egg yolks from laying hens supplemented with β-carotene. Qixiang Miao,Youyou Yang,Lihong Du,Chaohua Tang,Qingyu Zhao,Fadi Li,Xiao Yao,Ying Meng,Yuchang Qin,Junmin Zhang. 2023

[13]Comparative characterization of flavor precursors and volatiles of Taihe black-boned silky fowl and Hy-line Brown yolks using multiomics and GC-O-MS-based volatilomics. Weifang Yang,Youyou Yang,Liang Wang,Xueze Lv,Jing Li,Huanxian Cui,Chaohua Tang,Qingyu Zhao,Yaxiong Jia,Yuchang Qin,Junmin Zhang. 2023

[14]Modified EMR-lipid method combined with HPLC-MS/MS to determine folates in egg yolks from laying hens supplemented with different amounts of folic acid. Dandan Sun,Yue Jin,Qingyu Zhao,Chaohua Tang,Yi Li,Hao Wang,Yuchang Qin,Junmin Zhang. 2021

[15]Modified EMR-lipid method combined with HPLC-MS/MS to determine folates in egg yolks from laying hens supplemented with different amounts of folic acid. Dandan Sun,Yue Jin,Qingyu Zhao,Chaohua Tang,Yi Li,Hao Wang,Yuchang Qin,Junmin Zhang. 2021

[16]Modified EMR-lipid method combined with HPLC-MS/MS to determine folates in egg yolks from laying hens supplemented with different amounts of folic acid. Dandan Sun,Yue Jin,Qingyu Zhao,Chaohua Tang,Yi Li,Hao Wang,Yuchang Qin,Junmin Zhang. 2021

[17]Comparative investigation of various protein or protein-based powders addition on texture properties of egg white and yolk gels. Dai, Yan,Cai, Guo-Xing,Liang, Yu,He, Zi-Ying,Zhang, Ying,Li, Chun-Hong,Chang, Hai-Jun. 2024

[18]Influence of chestnut tannins on welfare, carcass characteristics, meat quality, and lipid oxidation in rabbits under high ambient temperature. Liu, Huawei,Zhou, Daowei,Tong, Jianming,Vaddella, Venkata. 2012

[19]Colour Stability and Lipid Oxidation of Beef Longissimus Lumborum under Different Packaging Conditions. Bonny, Dadji Stephane Serge,Li, Xin,Li, Zheng,Li, Meng,Du, Manting,Gao, Lingling,Zhang, Dequan.

[20]Changes of Intramuscular Fat Composition, Lipid Oxidation and Lipase Activity in Biceps femoris and Semimembranosus of Xuanwei Ham During Controlled Salting Stages. Wang Zhen-yu,Zhang De-quan,Wang Zhen-yu,Gao Xiao-guang,Ma Chang-wei,Zhang Ji-hong. 2013

作者其他论文 更多>>