数字农科院2.0

Microbial inoculum improved soil aggregate formation and increased cucumber yield in a greenhouse under secondary salinization conditions

文献类型： 外文期刊

作者： Yuanyuan Peng;He Zhang;Guihua Li;Jianfeng Zhang

作者机构：

关键词： Bacillus;Extracellular polymeric substances;Inoculation;Saline soil;Soil aggregate;Trichoderma

期刊名称： Journal of Environmental Management

ISSN： 0301-4797

年卷期： 2025 年 376 卷

页码：

收录情况： SCIE（2025版） ; ; EI（2025版）

摘要： Greenhouse soil salinization caused by unreasonable fertilizer utilization and irrigation threatens vegetable growth and supply. Inoculation with salt-tolerant and plant growth-promoting microorganisms offers a sustainable solution. We screened eight Bacillus and two Trichoderma strains for salt tolerance using incubation method. Subsequent pot and column experiments were conducted to assess their impact on cucumber growth and soil aggregation. The two promising strains, Bacillus amyloliquefaciens 10225 and Trichoderma harzianum 32527, were selected for further validation in salinized greenhouse soil. Our findings revealed that both strains of B. amyloliquefaciens thrived in 5% NaCl solution and substantially promoted the growth of cucumber seedling growth. Compared with that in the control (non-inoculated treatment), large macroaggregate content increased by 65.12%, 52.60%, and 71.93% with B. amyloliquefaciens 19994, 10225, and T. harzianum 32527, respectively, and aggregate water mean weight diameter (WMWD) increased by 34.47%, 36.55%, and 43.68%. Extracellular polymeric substance (EPS) composition (protein, polysaccharide, and uranic acid contents) varied among strains, with protein and polysaccharide contents increasing with B. amyloliquefaciens 10270. The microbial biomass carbon (MBC) content also increased by 19.26% and 28.53% (p < 0.05) with B. amyloliquefaciens 10225 and 10270, respectively, compared with non-inoculated control. The cucumber yield increased by 14.73%–17.43% under inoculation (p < 0.05). Partial least squares path modeling (PLS–PM) revealed that EPS and MBC promoted soil aggregation under inoculation. Field experiments confirmed that inoculation significantly improved cucumber yield, which was attributed to an increase in soil aggregate stability and a decrease in soil electrical conductivity (EC). The study underscores the critical role of EPS and MBC in maintaining soil aggregation in saline soils post-inoculation with beneficial strains. These findings offer theoretical or practical insights for the use of microbial inoculation as a bio-organic fertilizer or bio-amendment in saline soils.

分类号：

  • 相关文献

[1]Microbial inoculation improves soil aggregation by enhancing exopolysaccharides and lipopolysaccharides-related gene abundance in saline soil. Yuanyuan Peng,He Zhang,Zuopeng Lv,Jianfeng Zhang,Guihua Li. 2025

[2]利用ubil内含子增强Btcry1Ah基因在转基因玉米中的表达. 郎志宏,张杰,何康来,宋福平,黄大防. 2008

[3]Effect of inoculation with Penicillium expansum on the microbial community and maturity of compost. Wang, Hong-yuan,Fan, Bing-quan,Hu, Qing-xiu,Yin, Zhong-wei.

[4]Evaluation of oyster mushroom strains for resistance to Pseudomonas tolaasii by inoculation in spawned substrates. Zhang, Rui Ying,Gu, Jin Gang,Zuo, Xue Mei,Hu, Qing Xiu,Zhang, Jin Xia,Zhang, Rui Ying,Gu, Jin Gang,Zuo, Xue Mei,Hu, Qing Xiu,Zhang, Jin Xia,Hu, Dan Dan.

[5]Exploring Potential Soil Bacteria For S.ustainable Wheat (Triticum Aestivum L .) Production. Sheirdil, RA, Hayat, R, Zhang, XX, Abbasi, NA, Ali, S, Ahmed, M, Khattak, JZK, Ahmad, S. 2019

[6]The microbial mechanisms of enhanced humification by inoculation with Phanerochaete chrysosporium and Trichoderma longibrachiatum during biogas residues composting. Jing He,Nengmin Zhu,Yansheng Xu,Li Wang,Jiaqiang Zheng,Xia Li. 2022

[7]Response of humification process to fungal inoculant in corn straw composting with two different kinds of nitrogen sources. Shijia Dong,Ruoqi Li,Kaiyun Zhou,Yuquan Wei,Jun Li,Meidi Cheng,Peizhen Chen,Xiaomei Hu. 2024

[8]Contribution of mono- and co-culture of Pseudomonas paralactis, Acinetobacter MN21 and Stenotrophomonas maltophilia to the spoilage of chill-stored lamb. Xiangyuan Wen,Dequan Zhang,James D. Morton,Su Wang,Xiaoyu Chai,Xin Li,Qingfeng Yang,Jinhuo Li,Wei Yang,Chengli Hou. 2024

[9]Increase in the Maturity of Walnut Green Husk Compost by Adding a Microbial Consortium with High Tolerance to Walnut Green Husk Extract. Dai, Jinping,He, Jing,Zhao, Xi,Yao, Rui,Chen, Yifu,Wang, Li,Li, Xia. 2025

[10]Evaluation of in situ spoilage ability of common facultative anaerobic bacteria and confirmation of volatile spoilage markers in vacuum-packaged lamb. Xiangyuan Wen,Dequan Zhang,Su Wang,Zhiyuan Xiao,Xin Li,Debao Wang,Wei Yang,Chengjun Min,Chengli Hou. 2025

[11]Use of formulated Trichoderma sp Tri-1 in combination with reduced rates of chemical pesticide for control of Sclerotinia sclerotiorium on oilseed rape. Hu, Xiaojia,Xie, Lihua,Yu, Changbing,Li, Yinshui,Qin, Lu,Hu, Lei,Zhang, Yinbo,Liao, Xing,Roberts, Daniel P.. 2015

[12]Biodegradation of neonicotinoid insecticide, imidacloprid by restriction enzyme mediated integration (REMI) generated Trichoderma mutants. He, Xiangfeng,Wubie, Abebe Jenberie,Diao, Qingyun,Li, Wei,Xue, Fei,Guo, Zhanbo,Zhou, Ting,Xu, Shufa,Wubie, Abebe Jenberie.

[13]A new method for the preservation of axenic fungal cultures. Hu, Xiaojia,Xie, Lihua,Yu, Changbing,Li, Yinshui,Liao, Xing,Webster, Gordon. 2014

[14]The Effects Of Trichoderma On P.reventing Cucumber Fusarium Wilt A nd Regulating Cucumber Physiology. Li Mei,Lian Hua,Tian Ying,Su Xiao-lin,Ma Guang-shu,Huang Wen-kun,Mei Jie,Jiang Xi-liang. 2019

[15]Trichoderma Biodiversity In Major Ecological S.ystems Of China. Dou, K, Gao, JX, Zhang, CL, Yang, HT, Jiang, XL, Li, JS, Li, YQ, Wang, W, Xian, HQ, Li, SG, Liu, Y, Hu, JD, Chen, J. 2019

[16]Trichoderma and its role in biological control of plant fungal and nematode disease. Yao, Xin,Guo, Hailin,Zhang, Kaixuan,Zhao, Mengyu,Ruan, Jingjun,Chen, Jie. 2023

[17]The volatile cedrene from Trichoderma guizhouense modulates Arabidopsis root development through auxin transport and signalling. Li, Yucong,Shao, Jiahui,Fu, Yansong,Chen, Yu,Wang, Hongzhe,Xu, Zhihui,Feng, Haichao,Xun, Weibing,Liu, Yunpeng,Zhang, Nan,Shen, Qirong,Xuan, Wei,Zhang, Ruifu. 2021

[18]Exploring the multi-level regulation of lignocellulases in the filamentous fungus Trichoderma guizhouense NJAU4742 from an omics perspective. Xia, Yanwei,Wang, Jingfan,Guo, Chuanxu,Xu, Huanhuan,Wang, Wei,Yang, Mingzhu,Shen, Qirong,Zhang, Ruifu,Miao, Youzhi. 2022

[19]Factors Underlying the Prevalence of Pythium Infection of Corn Seeds Following Seed Treatment Application of Tebuconazole. Xiujun Tang,Shuning Chen,Xiaojing Yan,Zhenying Wang,Huizhu Yuan,Daibin Yang. 2022

[20]Potential of Trichoderma spp. for Biocontrol of Aflatoxin-Producing Aspergillus flavus. Xianfeng Ren,Maria Teresa Branà,Miriam Haidukowski,Antonia Gallo,Qi Zhang,Antonio F. Logrieco,Peiwu Li,Shancang Zhao,Claudio Altomare. 2022

作者其他论文 更多>>