数字农科院2.0

Biological nanoparticles from Brucella abortusΔeipB∆perΔwadC elicit protective immunity against brucellosis

文献类型： 外文期刊

作者： Yike Huang;Hengtai Wang;Xiaowei Peng;Tianpeng Li;Xuezheng Fan;Qingchun Shen;Guangzhi Zhang;Qianlin Li;Pascal Boireau;Hui Jiang;Jiabo Ding;Peng Li

作者机构：

关键词： Brucella abortus;Brucellosis vaccine;Immune responses;Noncanonical LPS;Outer membrane vesicles

期刊名称： Journal of Nanobiotechnology

ISSN： 1477-3155

年卷期： 2025 年 23 卷 1 期

页码：

收录情况： SCIE（2025版） ; ; EI（2025版）

摘要： Abstract: Brucellosis, caused by bacteria of the genus Brucella, is a globally prevalent zoonotic disease that poses serious risks to both animal and human health. Brucella abortus releases the biological nanoparticles (outer-membrane-vesicles, OMVs), which contribute to chronic infection by promoting host invasion and immune evasion. In this study, we performed a detailed characterization of OMVs derived from the wild-type B. abortus strain. These OMVs enhanced bacterial invasion and intracellular survival in macrophages ex vivo and increased pathogenicity in mice by modulating macrophage polarization in vivo. However, native OMVs showed low yield and limited immunogenicity. To overcome these limitations, we engineered a recombinant B. abortus strain Bru-M3, by deleting the eipB, per, and wadC genes (Brucella abortus∆eipB∆per∆wadC), which remodels noncanonical lipopolysaccharide (LPS). The Bru-M3 strain produced OMVs (OMVBru−M3) at significantly higher yields and with enhanced immunogenicity. Immunization of mice via subcutaneous or intranasal routes with OMVBru−M3 conferred better effective protection against both low and high-dose intraperitoneal challenges with wild-type B. abortus. Vaccination with OMVBru−M3 also elicited stronger humoral responses and IFN-γ–producing Th1 CD4⁺ T cells, resulting in improved bacterial clearance, reduced tissue damage, and elevated inflammatory cytokine production compared to OMVs from wild-type B. abortus. These findings support the potential of OMVBru−M3 as a promising candidate for brucellosis vaccine development. Graphic abstract: (Figure presented.)

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