数字农科院2.0

Development and validation of signature peptide-based isotope dilution liquid chromatography-mass spectrometry for bovine lactoferrin purity assessment

文献类型： 外文期刊

作者： Yan; Jingjing;Yang; Mengrui;Wang; Min;Han; Yiyi;Zhou; Jian;Ma; Yingqing;Wang; Tongtong;Li; Liang

作者机构：

关键词： Bovine lactoferrin;Signature peptide;Isotope dilution mass spectrometry;Protein quantification;Active concentration

期刊名称： MICROCHEMICAL JOURNAL

ISSN： 0026-265X

年卷期： 2025 年 208 卷

页码：

收录情况： SCIE（2025版）

摘要： A bovine lactoferrin (bLF) quantification method using signature peptide-based IDMS that is fully traceable to SI is presented. The two stages IDMS method's two stages include quantifying the synthetic peptide by AA-based IDMS via acid hydrolysis and the bLF certification by signature peptide-based IDMS via enzyme digestion. The purity of signature peptide was assessed to be 96.7 % with an uncertainty of 1.95 %, and the bLF was determined to be 0.941 +/- 0.024 g/g. The specificity, linearity, and uncertainty are assessed. The quantitative result of the present method is consistent with previous mass balance method (0.938 +/- 0.011 g/g) and AA-based IDMS (0.937 +/- 0.027 g/g) results. The LOD and LOQ were 35 ug/g and 117 ug/g, respectively, and the uncertainties resulting from the analysis processes are evaluated. Furthermore, we determined bLF in infant formulas using the developed IDMS method and standard HPLC method, and their quantitative results bias is due to the measurement of active concentration and total concentration (ratio of 25 % to 49 %). The proposed method can benefit the development of relevant matrix certified reference materials.

分类号：

  • 相关文献

[1]Study of SI-traceable purity assessment of bovine lactoferrin using mass balance approach and amino acid-based isotope dilution liquid chromatography-mass spectrometry. Jingjing Yan,Min Wang,Mengrui Yang,Jian Zhou,Xiaofeng Xue,Tongtong Wang. 2022

[2]Comprehensive quantitation of multi-signature peptides originating from casein for the discrimination of milk from eight different animal species using LC-HRMS with stable isotope labeled peptides. Huiyan Zhang,Mohamed F. Abdallah,Jingjing Zhang,Yanan Yu,Qingyu Zhao,Chaohua Tang,Yuchang Qin,Junmin Zhang. 2022

[3]Research Progress in Isotope Labeling/Tags-Based Protein Quantification and Metrology Technologies. Wu, Fan,Zhang, Chenhuan,Chen, Rui,Chu, Zhanying,Han, Bin,Zhai, Rui. 2024

[4]Simultaneous Determination Of Four Triazines I.n Soil By Isotope D ilution Mass Spectrometry Using C-13-Labeled Triazines As Internal Standards. She, Dongmei,Jun, Ning,Wu, Changcai,Mei, Xiangdong,Quan, Haiyuan. 2019

[5]Study of a new matrix certified reference material for accurate measurement of florfenicol in prawn meat powder. Mengrui Yang,Lingbo Jian,Min Wang,Jian Zhou,Quanhui Liu,Tongtong Wang,Liyuan Zhang. 2021

[6]Contribution of bovine lactoferrin inter-lobe region to iron binding stability and antimicrobial activity against Staphylococcus aureus. Bai, Xuejing,Teng, Da,Tian, Zigang,Zhu, Yanping,Yang, Yalin,Wang, Jianhua,Bai, Xuejing,Teng, Da,Tian, Zigang,Zhu, Yanping,Yang, Yalin,Wang, Jianhua.

[7]Understanding the pH-dependent interaction of anthocyanin with two food-derived transferrins. Wenxin Wang,Peiqing Yang,Zhenzhen Xu,Liang Zhao,Yongtao Wang,Xiaojun Liao. 2023

[8]Effects of dietary supplementation of bovine lactoferrin on growth performance, immune function and intestinal health in weaning piglets. Ma, Xuanxuan,Hao, Ya,Mao, Ruoyu,Yang, Na,Zheng, Xueling,Li, Bing,Wang, Zhenlong,Zhang, Qingjuan,Teng, Da,Wang, Jianhua. 2022

[9]Effects of a Novel Bovine Lactoferrin-Derived Peptide on the Intestinal Morphology and Intestinal Flora in Rats. Huang, Tianle,Yang, Huan,Zhao, Yang,Cui, Haiyue,Qi, Xiaoxi,Miao, Liguang. 2025

[10]Treatment of lactoferrin and antimicrobial peptide N6 on bacterial enteritis caused by Escherichia coli in mice. Ma, Xuanxuan,Zhang, Kun,Yang, Na,Hao, Ya,Mao, Ruoyu,Teng, Da,Wang, Jianhua. 2024

[11]In vitro efficacy of bovine lactoferrin against bovine viral diarrhea virus. Hongwei Cui,Songda Li,Xiaohuan Yan,Zhaohua Wang,Tianyi Leng,Ming Li,Songli Li. 2025

作者其他论文 更多>>