数字农科院2.0

Comparative transcriptome analysis reveals genes involved in trichome development and metabolism in tobacco

文献类型： 外文期刊

作者： Mingli Chen;Zhiyuan Li;Xinxi He;Zhe Zhang;Dong Wang;Luying Cui;Minmin Xie;Zeyu Zhao;Quan Sun;Dahai Wang;Jiameng Dai;Daping Gong

作者机构：

关键词： Development;Glandular trichomes;Secondary metabolism;Tobacco;Transcription factors

期刊名称： BMC Plant Biology

ISSN： 1471-2229

年卷期： 2024 年 24 卷 1 期

页码：

收录情况： SCIE（2024版）

摘要： Background: The glandular trichomes of tobacco (Nicotiana tabacum) can efficiently produce secondary metabolites. They act as natural bioreactors, and their natural products function to protect plants against insect-pests and pathogens and are also components of industrial chemicals. To clarify the molecular mechanisms of tobacco glandular trichome development and secondary metabolic regulation, glandular trichomes and glandless trichomes, as well as other different developmental tissues, were used for RNA sequencing and analysis. Results: By comparing glandless and glandular trichomes with other tissues, we obtained differentially expressed genes. They were obviously enriched in KEGG pathways, such as cutin, suberine, and wax biosynthesis, flavonoid and isoflavonoid biosynthesis, terpenoid biosynthesis, and plant–pathogen interaction. In particular, the expression levels of genes related to the terpenoid, flavonoid, and wax biosynthesis pathway mainly showed down-regulation in glandless trichomes, implying that they lack the capability to synthesize certain exudate compounds. Among the differentially expressed genes, 234 transcription factors were found, including AP2-ERFs, MYBs, bHLHs, WRKYs, Homeoboxes (HD-ZIP), and C2H2-ZFs. These transcription factor and genes that highly expressed in trichomes or specially expressed in GT or GLT. Following the overexpression of R2R3-MYB transcription factor Nitab4.5_0011760g0030.1 in tobacco, an increase in the number of branched glandular trichomes was observed. Conclusions: Our data provide comprehensive gene expression information at the transcriptional level and an understanding of the regulatory pathways involved in glandular trichome development and secondary metabolism.

分类号：

  • 相关文献

[1]NtPHYB1(K326), a homologous gene of Arabidopsis PHYB, positively regulates the content of phenolic compounds in tobacco. Zhao, Jiehong,Zhang, Jie,Li, Zhenhua,Yu, Jing,Yu, Shizhou,Guo, Yushuang,Fu, Yongfu,Zhang, Xiaomei,Han, Jie.

[2]Isolation and Characterization of an ERF Transcription Factor Gene from Cotton (Gossypium barbadense L.). Xianpeng Meng,Fuguang Li,Chuanliang Liu,Chaojun Zhang,Zhixia Wu,Yajuan Chen.

[3]Single-cell transcriptome sequencing reveals the sphingolipid metabolism pathway plays an important role in leaf senescence in tobacco. Quan Sun,Bing Hou,Xinghui Liu,Langlang Zhang,Pingan Chang,Huimin Pang,Xiaohong He,Zexiang Huang,Jili Zhang,Guoxia Liu,Daping Gong,Mingli Chen. 2025

[4]烟草根际解钾茵的筛选与鉴定. 刘璇,孔凡玉,张成省,王静,冯超,赵杰. 2012

[5]Selective Toxicity of Secondary Metabolites from the Entomopathogenic Bacterium Photorhabdus luminescens sonorensis against Selected Plant Parasitic Nematodes of the Tylenchina Suborder. Kusakabe A., 王辰., Xu Y.-M., Molnár I., Stock S.P.. 2022

[6]Effects of Carbon Concentrations and Carbon to Nitrogen ratios on Sporulation of Two Biological Control Fungi as Determined by Different Culture Methods. Liu, Xingzhong,Gao, Li.

[7]Nitric oxide mediates brassinosteroid-induced flavonoid biosynthesis in Camellia sinensis L.. Li, Xin,Zhang, Lan,Ahammed, Golam Jalal,Li, Zhi-Xin,Wei, Ji-Peng,Shen, Chen,Yan, Peng,Zhang, Li-Ping,Han, Wen-Yan,Ahammed, Golam Jalal,Wei, Ji-Peng,Shen, Chen.

[8]Brassinosteroids Improve Quality of Summer Tea (Camellia sinensis L.) by Balancing Biosynthesis of Polyphenols and Amino Acids. Li, Xin,Ahammed, Golam J.,Li, Zhi-Xin,Zhang, Lan,Wei, Ji-Peng,Shen, Chen,Yan, Peng,Zhang, Li-Ping,Han, Wen-Yan,Ahammed, Golam J.,Li, Zhi-Xin,Shen, Chen. 2016

[9]Biochemical and Molecular Characterization of Plant MYB Transcription Factor Family. Du, Hai,Liu, Lei,Tang, Xiao-Feng,Huang, Yu-Bi,Du, Hai,Zhang, Li,Liu, Lei,Tang, Xiao-Feng,Yang, Wen-Jie,Wu, Yan-Min,Tang, Yi-Xiong.

[10]Flavonoid Accumulation In Spontaneous Cotton M.utant Results In Red C oloration And Enhanced Disease Resistance. Gao, Ya,Long, Lu,Liu, Ji,Gao, Wei,Xu, Fu:Chun,Zhao, Jing:Ruo,Li, Bing. 2019

[11]Complete genome sequence of Pseudomonas azotoformans S4, a potential biocontrol bacterium. Fang, Yang,Wu, Lijuan,Chen, Guoqing,Feng, Guozhong,Fang, Yang.

[12]Genome sequence of Pseudomonas parafulva CRS01-1, an antagonistic bacterium isolated from rice field. Liu, Qunen,Zhang, Yingxin,Yu, Ning,Bi, Zhenzhen,Zhu, Aike,Zhan, Xiaodeng,Wu, Weixun,Yu, Ping,Chen, Daibo,Cheng, Shihua,Cao, Liyong,Zhang, Yingxin,Zhan, Xiaodeng,Wu, Weixun,Yu, Ping,Chen, Daibo,Cheng, Shihua,Cao, Liyong.

[13]Whole genome sequence of Pantoea ananatis R100, an antagonistic bacterium isolated from rice seed. Wu, Liwen,Liu, Ruifang,Niu, Yaofang,Lin, Haiyan,Ye, Weijun,Guo, Longbiao,Hu, Xingming,Liu, Ruifang,Lin, Haiyan.

[14]Genetic and metabolic engineering of isoflavonoid biosynthesis. Du, Hai,Huang, Yubi,Du, Hai,Tang, Yixiong. 2010

[15]Whole genome sequence of Pseudomonas aeruginosa F9676, an antagonistic bacterium isolated from rice seed. Shi, Zhenyuan,Ren, Deyong,Hu, Shikai,Hu, Xingming,Wu, Liwen,Lin, Haiyan,Hu, Jiang,Zhang, Guangheng,Guo, Longbiao,Hu, Shikai,Lin, Haiyan.

[16]Glucosinolate biosynthetic genes in Brassica rapa. Wang, Xiaowu. 2011

[17]Genome Sequence of Pseudomonas koreensis CRS05-R5, an Antagonistic Bacterium Isolated from Rice Paddy Field. Haiyan Lin , Shikai Hu +, Ruifang Liu , Ping Chen , Changwei Ge , Bo Zhu , Longbiao Guo *. 2016

[18]Integration of Transcriptomic and Metabolomic Profiles Provides Insights into the Influence of Nitrogen on Secondary Metabolism in Fusarium sacchari. Yixue Bao,Zhenyue Lin,Wei Yao,Sehrish Akbar,Wenfeng Lin,Charles A. Powell,Jianlong Xu,Muqing Zhang. 2023

[19]Analysis of metabolic differences in maize in different growth stages under nitrogen stress based on UPLC-QTOF-MS. Guipei Song,Yanli Lu,Yuhong Wang,Caie Nie,Mengze Xu,Lei Wang,Youlu Bai. 2023

[20]Homoeolog gene expression analysis reveals novel expression biases in upland hybrid cotton under intraspecific hybridization. Shahzad, Kashif,Zhang, Xuexian,Zhang, Meng,Guo, Liping,Qi, Tingxiang,Tang, Huini,Wang, Hailin,Mubeen, Iqra,Qiao, Xiuqin,Peng, Renhai,Wu, Jianyong,Xing, Chaozhu. 2022

作者其他论文 更多>>