数字农科院2.0

A novel phytase from Yersinia rohdei with high phytate hydrolysis activity under low pH and strong pepsin conditions

文献类型： 外文期刊

作者： Huang, Huoqing;Luo, Huiying;Wang, Yaru;Fu, Dawei;Shao, Na;Wang, Guozeng;Yang, Peilong;Yao, Bin

作者机构：

关键词： gastric conditions;inorganic phosphorus;phytase;Yersinia rohdei;Y. pestis;SITE-DIRECTED MUTAGENESIS;SOYBEAN MEAL DIETS;MICROBIAL PHYTASE;SACCHAROMYCES-CEREVISIAE;PICHIA-PASTORIS;WEANLING PIGS;YOUNG-PIGS;PHOSPHORUS;THERMOSTABILITY;BIOAVAILABILITY

期刊名称： APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Two novel phytase genes belonging to the histidine acid phosphatase family were cloned from Yersinia rohdei and Y. pestis and expressed in Pichia pastoris. Both the recombinant phytases had high activity at pH 1.5-6.0 (optimum pH 4.5) with an optimum temperature of 55 degrees C. Compared with the major commercial phytases from Aspergillus niger, Escherichia coli, and a potential commercial phytase from Y. intermedia, the Y. rohdei phytase was more resistant to pepsin, retained more activity under gastric conditions, and released more inorganic phosphorus (two to ten times) from soybean meal under simulated gastric conditions. These superior properties suggest that the Y. rohdei phytase is an attractive additive to animal feed. Our study indicated that, in order to better hydrolyze the phytate and release more inorganic phosphorus in the gastric passage, phytase should have high activity and stability, simultaneously, at low pH and high protease concentration.

分类号： Q93

  • 相关文献

[1]Cloning of beta-1,3-1,4-glucanase gene from Bacillus licheniformis EGW039 (CGMCC 0635) and its expression in Escherichia coli BL21 (DE3). Teng, Da,Wang, Jian-Hua,Fan, Ying,Yang, Ya-lin,Tian, Zi-gang,Luo, Jin,Yang, Guan-pin,Zhang, Fan.

[2]Improvement of Yersinia frederiksenii Phytase Performance by a Single Amino Acid Substitution. Fu, Dawei,Huang, Huoqing,Meng, Kun,Wang, Yaru,Luo, Huiying,Yang, Peilong,Yuan, Tiezheng,Yao, Bin.

[3]Catalytic efficiency of HAP phytases is determined by a key residue in close proximity to the active site. Fu, Dawei,Li, Zhongyuan,Huang, Huoqing,Yuan, Tiezheng,Shi, Pengjun,Luo, Huiying,Meng, Kun,Yang, Peilong,Yao, Bin.

[4]Enhancing the Thermostability of Phytase to Boiling Point by Evolution-Guided Design. Wang, Qian,刘晓青,田健,Wang, Yaru,Zhang, Honglian,Wang, Yuan,Luo, Huiying,Yao, Bin,Huang, Huoqing,Tu, Tao. 2022

[5]Achieving thermostability of a phytase with resistance up to 100 °C. Tao Tu,Qian Wang,Ruyue Dong,Xiaoqing Liu,Leena Penttinen,Nina Hakulinen,Jian Tian,Wei Zhang,Yaru Wang,Huiying Luo,Bin Yao,Huoqing Huang. 2024

[6]Improvement of alkali stability and thermostability of Paenibacillus campinasensis Family-11 xylanase by directed evolution and site-directed mutagenesis. Zheng, Hongchen,Liu, Yihan,Han, Yang,Lu, Fuping,Zheng, Hongchen,Liu, Yihan,Sun, Mingzhe,Han, Yang,Wang, Jianling,Lu, Fuping,Zheng, Hongchen,Sun, Junshe,Liu, Yihan,Wang, Jianling,Sun, Mingzhe,Lu, Fuping.

[7]Improving the thermostability of a methyl parathion hydrolase by adding the ionic bond on protein surface. Su, Yidan,Tian, Jian,Wang, Ping,Chu, Xiaoyu,Wu, Ningfeng,Fan, Yunliu,Su, Yidan,Liu, Guoan.

[8]An intramolecular disulfide bond is required for the thermostability of methyl parathion hydrolase, OPHC2. Chu, Xiao-yu,Tian, Jian,Wu, Ning-feng,Fan, Yun-liu.

[9]Improvement of thermostability and catalytic efficiency of glucoamylase from Talaromyces leycettanus JCM12802 via site-directed mutagenesis to enhance industrial saccharification applications. Lige Tong,Jie Zheng,Xiao Wang,Xiaolu Wang,Huoqing Huang,Haomeng Yang,Tao Tu,Yuan Wang,Yingguo Bai,Bin Yao,Huiying Luo,Xing Qin. 2021

[10]Enhanced enzyme thermostability of a family I.3 lipase LipSR1 by T118A mutation at the calcium-binding site. Jiang, Shijie,Zhou, Zhengfu,Han, Jiahui,Fan, Qingfeng,Long, Zhijian,Wang, Jin. 2023

[11]Measurement of total tract retention of phosphorus in several feedstuffs of plant origin for broiler chickens: effect of microbial phytase. Tesfay Hagos Haile,Liu Guohua,Zhengke Wu,Jiang Chen,Huakang Wang,Shoaib Ahmed Pirzado,Cai Huiyi. 2021

[12]The impact of the interaction between dietary total phosphorus level and efficacy of phytase on the performance of growing-finishing pigs. Cao, S. C.,Gong, J.,Wang, J.,Yan, H. L.,Zhang, H. F.,Liu, J. B.. 2023

[13]Effects of total calcium-to-total phosphorus ratio on the calcium and phosphorus digestibility and growth performance of growing pigs. S. C. Cao,J. F. Zhao,P. C. Xue,H. X. Zhai,L. Chen,H. F. Zhang,J. B. Liu. 2025

[14]The positive effects of super-dosing phytase on the growth performance of growing pigs are mainly due to increased dietary digestible phosphorus. Zhao, Jianfei,Cao, Shanchuan,Yin, Heng,Xue, Pengcheng,Zhai, Hengxiao,Chen, Liang,Zhang, Hongfu,Liu, Jingbo. 2025

[15]Effect of bone char application on Pb bioavailability in a Pb-contaminated soil. Chen, SB,Zhu, YG,Ma, YB,McKay, G.

[16]磷肥对苜蓿根系发育的影响. 韩雪松. 2002

[17]Growth promoting effect of a transgenic Bacillus mucilaginosus on tobacco planting. Li, Xin,Wu, Zhiqiang,Li, Weidong,Yan, Ruixiang,Li, Li,Li, Jun,Li, Yihang,Li, Minggang.

[18]Dietary microbial phytase exerts mixed effects on the gut health of tilapia: a possible reason for the null effect on growth promotion. Hu, Jun,Zhang, Xuezhen,Hu, Jun,Ran, Chao,He, Suxu,Cao, Yanan,Yao, Bin,Zhou, Zhigang,Ye, Yuantu.

[19]Eukaryotic expression and antimicrobial spectrum determination of the peptide tachyplesin II. Xu, Feng,Meng, Kun,Wang, Ya-Ru,Lu, Hui-Ying,Yang, Pei-Long,Yao, Bin,Xu, Feng,Wu, Ning-Feng,Fan, Yun-Liu.

[20]Cloning, expression, and characterization of a new xylanase with broad temperature adaptability from Streptomyces sp S9. Li, Ning,Meng, Kun,Wang, Yaru,Shi, Pengjun,Luo, Huiying,Bai, Yingguo,Yang, Peilong,Yao, Bin.

作者其他论文 更多>>