数字农科院2.0

Infective viruses produced from full-length complementary DNA of swine vesicular disease viruses HK/70 strain

文献类型： 外文期刊

作者： Zheng Haixue;Liu Xinagtao;Shang Youjun;Wu Jinyan;Bai Xingwen;Jin Ye;Sun Shiqi;Guo Huichen;Tian Hong;Feng Xia;Yin Shaunghui;Guo Jianhong;Cong Guozheng;Liu Zaixin;Chang Huiyun;Ma Junwu;Xie Qingge

作者机构：

关键词： swine vesicular disease virus;full-length cDNA;infectious clone;pSVOK_(12);reverse genetics

期刊名称： CHINESE SCIENCE BULLETIN

ISSN：

年卷期：

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收录情况： SCI

摘要： The full-length cDNA clone of swine vesicular disease virus HK/70 strain named pSVOK_(12) was constructed in order to study the antigenicity, replication, maturation and pathogenicity of swine vesicular disease virus. In vitro transcription RNA from pSVOK_(12) transfected IBRS-2 cells and the recovered virus RNA were isolated and sequenced, then indirect hemagglutination test, indirect im-munofluorescence assays, eleectron microscope test, 50 percent tissue culture infecting dose (TCID_(50)) assays and mouse virulence studies were performed to study the antigenicity and virulence of the recovered virus. The result showed that the infectious clones we obtained and the virus derived from pSVOK_(12) had the ' same biological properties as the parental strain HK/70. The full-length infectious cDNA clone, pSVOK_(12), will be very useful in studies of the antigenicity, virulence, pathogenesis, maturation and replication of SVDV.

分类号： N1

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